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Detection of Exosomal miRNAs in the Plasma of Melanoma Patients

MicroRNAs (miRNAs) are a class of 22–25 nucleotide RNAs that control gene expression at the post-transcriptional level. MiRNAs have potential as cancer biomarkers. Melanoma is a highly aggressive form of skin cancer accounting for almost 4% of cancers among men and women, and ~80% of skin cancer-rel...

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Autores principales: Pfeffer, Susan R., Grossmann, Kenneth F., Cassidy, Pamela B., Yang, Chuan He, Fan, Meiyun, Kopelovich, Levy, Leachman, Sancy A., Pfeffer, Lawrence M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4693157/
https://www.ncbi.nlm.nih.gov/pubmed/26694476
http://dx.doi.org/10.3390/jcm4121957
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author Pfeffer, Susan R.
Grossmann, Kenneth F.
Cassidy, Pamela B.
Yang, Chuan He
Fan, Meiyun
Kopelovich, Levy
Leachman, Sancy A.
Pfeffer, Lawrence M.
author_facet Pfeffer, Susan R.
Grossmann, Kenneth F.
Cassidy, Pamela B.
Yang, Chuan He
Fan, Meiyun
Kopelovich, Levy
Leachman, Sancy A.
Pfeffer, Lawrence M.
author_sort Pfeffer, Susan R.
collection PubMed
description MicroRNAs (miRNAs) are a class of 22–25 nucleotide RNAs that control gene expression at the post-transcriptional level. MiRNAs have potential as cancer biomarkers. Melanoma is a highly aggressive form of skin cancer accounting for almost 4% of cancers among men and women, and ~80% of skin cancer-related deaths in the US. In the present study we analyzed plasma-derived exosomal miRNAs from clinically affected and unaffected familial melanoma patients (CDKN2A/p16 gene carriers) and compared them with affected (nonfamilial melanoma) and unaffected control subjects in order to identify novel risk biomarkers for melanoma. Intact miRNAs can be isolated from the circulation because of their presence in exosomes. A number of differentially regulated miRNAs identified by NanoString human V2 miRNA array were validated by quantitative PCR. Significantly, miR-17, miR-19a, miR-21, miR-126, and miR-149 were expressed at higher levels in patients with metastatic sporadic melanoma as compared with familial melanoma patients or unaffected control subjects. Surprisingly, no substantial differences in miRNA expression were detected between familial melanoma patients (all inclusive) and unaffected control subjects. The miRNAs differentially expressed in the different patient cohorts, especially in patients with metastatic melanoma, may play important roles in tumor progression and metastasis, and may be used as predictive biomarkers to monitor remission as well as relapse following therapeutic intervention.
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spelling pubmed-46931572016-01-06 Detection of Exosomal miRNAs in the Plasma of Melanoma Patients Pfeffer, Susan R. Grossmann, Kenneth F. Cassidy, Pamela B. Yang, Chuan He Fan, Meiyun Kopelovich, Levy Leachman, Sancy A. Pfeffer, Lawrence M. J Clin Med Article MicroRNAs (miRNAs) are a class of 22–25 nucleotide RNAs that control gene expression at the post-transcriptional level. MiRNAs have potential as cancer biomarkers. Melanoma is a highly aggressive form of skin cancer accounting for almost 4% of cancers among men and women, and ~80% of skin cancer-related deaths in the US. In the present study we analyzed plasma-derived exosomal miRNAs from clinically affected and unaffected familial melanoma patients (CDKN2A/p16 gene carriers) and compared them with affected (nonfamilial melanoma) and unaffected control subjects in order to identify novel risk biomarkers for melanoma. Intact miRNAs can be isolated from the circulation because of their presence in exosomes. A number of differentially regulated miRNAs identified by NanoString human V2 miRNA array were validated by quantitative PCR. Significantly, miR-17, miR-19a, miR-21, miR-126, and miR-149 were expressed at higher levels in patients with metastatic sporadic melanoma as compared with familial melanoma patients or unaffected control subjects. Surprisingly, no substantial differences in miRNA expression were detected between familial melanoma patients (all inclusive) and unaffected control subjects. The miRNAs differentially expressed in the different patient cohorts, especially in patients with metastatic melanoma, may play important roles in tumor progression and metastasis, and may be used as predictive biomarkers to monitor remission as well as relapse following therapeutic intervention. MDPI 2015-12-17 /pmc/articles/PMC4693157/ /pubmed/26694476 http://dx.doi.org/10.3390/jcm4121957 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pfeffer, Susan R.
Grossmann, Kenneth F.
Cassidy, Pamela B.
Yang, Chuan He
Fan, Meiyun
Kopelovich, Levy
Leachman, Sancy A.
Pfeffer, Lawrence M.
Detection of Exosomal miRNAs in the Plasma of Melanoma Patients
title Detection of Exosomal miRNAs in the Plasma of Melanoma Patients
title_full Detection of Exosomal miRNAs in the Plasma of Melanoma Patients
title_fullStr Detection of Exosomal miRNAs in the Plasma of Melanoma Patients
title_full_unstemmed Detection of Exosomal miRNAs in the Plasma of Melanoma Patients
title_short Detection of Exosomal miRNAs in the Plasma of Melanoma Patients
title_sort detection of exosomal mirnas in the plasma of melanoma patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4693157/
https://www.ncbi.nlm.nih.gov/pubmed/26694476
http://dx.doi.org/10.3390/jcm4121957
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