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Antibacterial efficacy of Azadirachta indica, Mimusops elengi and 2% CHX on multispecies dentinal biofilm

AIMS: To check the antimicrobial activity of Azadirachta indica (Neem), Mimusops elengi (Bakul), and Chlorhexidine gluconate (CHX) on multispecies biofilm of common endodontic pathogens such as Streptococcus mutans, Enterococcus faecalis, Staphylococcus aureus and Candida albicans. SETTINGS AND DESI...

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Autores principales: Mistry, Kunjal S., Sanghvi, Zarna, Parmar, Girish, Shah, Samir, Pushpalatha, Kasukurthi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4693319/
https://www.ncbi.nlm.nih.gov/pubmed/26752840
http://dx.doi.org/10.4103/0972-0707.168810
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author Mistry, Kunjal S.
Sanghvi, Zarna
Parmar, Girish
Shah, Samir
Pushpalatha, Kasukurthi
author_facet Mistry, Kunjal S.
Sanghvi, Zarna
Parmar, Girish
Shah, Samir
Pushpalatha, Kasukurthi
author_sort Mistry, Kunjal S.
collection PubMed
description AIMS: To check the antimicrobial activity of Azadirachta indica (Neem), Mimusops elengi (Bakul), and Chlorhexidine gluconate (CHX) on multispecies biofilm of common endodontic pathogens such as Streptococcus mutans, Enterococcus faecalis, Staphylococcus aureus and Candida albicans. SETTINGS AND DESIGN: In vitro dentin disinfection model used to check the antimicrobial efficacy of herbal extracts. MATERIALS AND METHODS: The in vitro dentin disinfection model was used to check the antimicrobial activity of the methanolic extracts of the medicinal plants along with Chlorhexidine gluconate. The polymicrobial biofilm was allowed to grow on extracted teeth sections for a period of 21 days. Remaining microbial load in the form of CFU/ml after the antimicrobial treatment was tabulated, and data were statistically analyzed using ANOVA and Bonferroni post-hoc tests. STATISTICAL ANALYSIS USED: SPSS version 17, one-way ANOVA, Bonferroni post-hoc test. RESULTS: Both the plant extracts showed considerable antimicrobial efficacy as compared to negative control. 2% CHX was the most effective antimicrobial agent having statistically significant difference against plant extracts and negative control (saline). CONCLUSION: The methanolic extract of A. Indica, M. elengi, and Chlorhexidine Gluconate has considerable antimicrobial activity against polymicrobial dentinal biofilm of S. mutans, E. faecalis, S. aureus and C. albicans.
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spelling pubmed-46933192016-01-08 Antibacterial efficacy of Azadirachta indica, Mimusops elengi and 2% CHX on multispecies dentinal biofilm Mistry, Kunjal S. Sanghvi, Zarna Parmar, Girish Shah, Samir Pushpalatha, Kasukurthi J Conserv Dent Original Article AIMS: To check the antimicrobial activity of Azadirachta indica (Neem), Mimusops elengi (Bakul), and Chlorhexidine gluconate (CHX) on multispecies biofilm of common endodontic pathogens such as Streptococcus mutans, Enterococcus faecalis, Staphylococcus aureus and Candida albicans. SETTINGS AND DESIGN: In vitro dentin disinfection model used to check the antimicrobial efficacy of herbal extracts. MATERIALS AND METHODS: The in vitro dentin disinfection model was used to check the antimicrobial activity of the methanolic extracts of the medicinal plants along with Chlorhexidine gluconate. The polymicrobial biofilm was allowed to grow on extracted teeth sections for a period of 21 days. Remaining microbial load in the form of CFU/ml after the antimicrobial treatment was tabulated, and data were statistically analyzed using ANOVA and Bonferroni post-hoc tests. STATISTICAL ANALYSIS USED: SPSS version 17, one-way ANOVA, Bonferroni post-hoc test. RESULTS: Both the plant extracts showed considerable antimicrobial efficacy as compared to negative control. 2% CHX was the most effective antimicrobial agent having statistically significant difference against plant extracts and negative control (saline). CONCLUSION: The methanolic extract of A. Indica, M. elengi, and Chlorhexidine Gluconate has considerable antimicrobial activity against polymicrobial dentinal biofilm of S. mutans, E. faecalis, S. aureus and C. albicans. Medknow Publications & Media Pvt Ltd 2015 /pmc/articles/PMC4693319/ /pubmed/26752840 http://dx.doi.org/10.4103/0972-0707.168810 Text en Copyright: © 2015 Journal of Conservative Dentistry http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Mistry, Kunjal S.
Sanghvi, Zarna
Parmar, Girish
Shah, Samir
Pushpalatha, Kasukurthi
Antibacterial efficacy of Azadirachta indica, Mimusops elengi and 2% CHX on multispecies dentinal biofilm
title Antibacterial efficacy of Azadirachta indica, Mimusops elengi and 2% CHX on multispecies dentinal biofilm
title_full Antibacterial efficacy of Azadirachta indica, Mimusops elengi and 2% CHX on multispecies dentinal biofilm
title_fullStr Antibacterial efficacy of Azadirachta indica, Mimusops elengi and 2% CHX on multispecies dentinal biofilm
title_full_unstemmed Antibacterial efficacy of Azadirachta indica, Mimusops elengi and 2% CHX on multispecies dentinal biofilm
title_short Antibacterial efficacy of Azadirachta indica, Mimusops elengi and 2% CHX on multispecies dentinal biofilm
title_sort antibacterial efficacy of azadirachta indica, mimusops elengi and 2% chx on multispecies dentinal biofilm
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4693319/
https://www.ncbi.nlm.nih.gov/pubmed/26752840
http://dx.doi.org/10.4103/0972-0707.168810
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