Evaluation of effect of addition of 2% chlorhexidine on the sealing ability of Biodentine: An in vitro study

AIM: To evaluate the effect of the addition of 2% chlorhexidine on the sealing ability of Biodentine. MATERIALS AND METHODS: Forty-six extracted human premolar teeth with single canal and apical foramen were selected, cleaned, and decoronated to standardize the length of 17 mm. Canals were prepared using ProTaper rotary files till size F4. The samples were divided into 2 experimental groups of 20 samples each on the basis of absence/presence of 2% chlorhexidine in liquid: Group B(M) = Biodentine mixed with the provided liquid, Group B(C) = Biodentine mixed with 2% chlorhexidine in provided liquid. Three samples, each were assigned to control groups: Group B(P) (positive control) = No root end filling was placed, Group B(N) (negative control) = Root ends were filled as in Group B(M,) and entire external surface was coated with sticky wax. The samples were then evaluated for the apical sealing using fluid filtration method. RESULTS: Results were analyzed using Student's t-test (P ≤ 0.05). Group B(C) showed the better sealing ability (3.06) as compared to Group B(M) (3.85). However, the difference was statistically insignificant (P > 0.05). CONCLUSION: Addition of 2% chlorhexidine to the liquid enhanced the sealing ability of Biodentine.