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Circulating microRNA signature of genotype‐by‐age interactions in the long‐lived Ames dwarf mouse
Recent evidence demonstrates that serum levels of specific miRNAs significantly change with age. The ability of circulating sncRNAs to act as signaling molecules and regulate a broad spectrum of cellular functions implicates them as key players in the aging process. To discover circulating sncRNAs t...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4693471/ https://www.ncbi.nlm.nih.gov/pubmed/26176567 http://dx.doi.org/10.1111/acel.12373 |
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author | Victoria, Berta Dhahbi, Joseph M. Nunez Lopez, Yury O. Spinel, Lina Atamna, Hani Spindler, Stephen R. Masternak, Michal M. |
author_facet | Victoria, Berta Dhahbi, Joseph M. Nunez Lopez, Yury O. Spinel, Lina Atamna, Hani Spindler, Stephen R. Masternak, Michal M. |
author_sort | Victoria, Berta |
collection | PubMed |
description | Recent evidence demonstrates that serum levels of specific miRNAs significantly change with age. The ability of circulating sncRNAs to act as signaling molecules and regulate a broad spectrum of cellular functions implicates them as key players in the aging process. To discover circulating sncRNAs that impact aging in the long‐lived Ames dwarf mice, we conducted deep sequencing of small RNAs extracted from serum of young and old mice. Our analysis showed genotype‐specific changes in the circulating levels of 21 miRNAs during aging [genotype‐by‐age interaction (GbA)]. Genotype‐by‐age miRNAs showed four distinct expression patterns and significant overtargeting of transcripts involved in age‐related processes. Functional enrichment analysis of putative and validated miRNA targets highlighted cellular processes such as tumor suppression, anti‐inflammatory response, and modulation of Wnt, insulin, mTOR, and MAPK signaling pathways, among others. The comparative analysis of circulating GbA miRNAs in Ames mice with circulating miRNAs modulated by calorie restriction (CR) in another long‐lived mouse suggests CR‐like and CR‐independent mechanisms contributing to longevity in the Ames mouse. In conclusion, we showed for the first time a signature of circulating miRNAs modulated by age in the long‐lived Ames mouse. |
format | Online Article Text |
id | pubmed-4693471 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-46934712016-01-04 Circulating microRNA signature of genotype‐by‐age interactions in the long‐lived Ames dwarf mouse Victoria, Berta Dhahbi, Joseph M. Nunez Lopez, Yury O. Spinel, Lina Atamna, Hani Spindler, Stephen R. Masternak, Michal M. Aging Cell Original Articles Recent evidence demonstrates that serum levels of specific miRNAs significantly change with age. The ability of circulating sncRNAs to act as signaling molecules and regulate a broad spectrum of cellular functions implicates them as key players in the aging process. To discover circulating sncRNAs that impact aging in the long‐lived Ames dwarf mice, we conducted deep sequencing of small RNAs extracted from serum of young and old mice. Our analysis showed genotype‐specific changes in the circulating levels of 21 miRNAs during aging [genotype‐by‐age interaction (GbA)]. Genotype‐by‐age miRNAs showed four distinct expression patterns and significant overtargeting of transcripts involved in age‐related processes. Functional enrichment analysis of putative and validated miRNA targets highlighted cellular processes such as tumor suppression, anti‐inflammatory response, and modulation of Wnt, insulin, mTOR, and MAPK signaling pathways, among others. The comparative analysis of circulating GbA miRNAs in Ames mice with circulating miRNAs modulated by calorie restriction (CR) in another long‐lived mouse suggests CR‐like and CR‐independent mechanisms contributing to longevity in the Ames mouse. In conclusion, we showed for the first time a signature of circulating miRNAs modulated by age in the long‐lived Ames mouse. John Wiley and Sons Inc. 2015-07-14 2015-12 /pmc/articles/PMC4693471/ /pubmed/26176567 http://dx.doi.org/10.1111/acel.12373 Text en © 2015 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Victoria, Berta Dhahbi, Joseph M. Nunez Lopez, Yury O. Spinel, Lina Atamna, Hani Spindler, Stephen R. Masternak, Michal M. Circulating microRNA signature of genotype‐by‐age interactions in the long‐lived Ames dwarf mouse |
title | Circulating microRNA signature of genotype‐by‐age interactions in the long‐lived Ames dwarf mouse |
title_full | Circulating microRNA signature of genotype‐by‐age interactions in the long‐lived Ames dwarf mouse |
title_fullStr | Circulating microRNA signature of genotype‐by‐age interactions in the long‐lived Ames dwarf mouse |
title_full_unstemmed | Circulating microRNA signature of genotype‐by‐age interactions in the long‐lived Ames dwarf mouse |
title_short | Circulating microRNA signature of genotype‐by‐age interactions in the long‐lived Ames dwarf mouse |
title_sort | circulating microrna signature of genotype‐by‐age interactions in the long‐lived ames dwarf mouse |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4693471/ https://www.ncbi.nlm.nih.gov/pubmed/26176567 http://dx.doi.org/10.1111/acel.12373 |
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