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HLA-DRB1*14 is a protective allele for multiple sclerosis in an admixed Colombian population

OBJECTIVE: The aim of this study was to determine ancestry informative markers, mitochondrial DNA haplogroups, and the association between HLA-DRB1 alleles and multiple sclerosis (MS) in a group of patients from Bogotá, Colombia. METHODS: In this case-control study, genomic DNA was isolated and puri...

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Autores principales: Toro, Jaime, Cuellar-Giraldo, David, Díaz-Cruz, Camilo, Burbano, Lisseth-Estefania, Guío, Claudia-Marcela, Reyes, Saúl, Cortes, Fabián, Cárdenas-Robledo, Simón, Narváez, Diana M., Cárdenas, Wilmer, Porras, Alexandra, Lattig, María-Claudia, Groot de Restrepo, Helena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694072/
https://www.ncbi.nlm.nih.gov/pubmed/26740965
http://dx.doi.org/10.1212/NXI.0000000000000192
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author Toro, Jaime
Cuellar-Giraldo, David
Díaz-Cruz, Camilo
Burbano, Lisseth-Estefania
Guío, Claudia-Marcela
Reyes, Saúl
Cortes, Fabián
Cárdenas-Robledo, Simón
Narváez, Diana M.
Cárdenas, Wilmer
Porras, Alexandra
Lattig, María-Claudia
Groot de Restrepo, Helena
author_facet Toro, Jaime
Cuellar-Giraldo, David
Díaz-Cruz, Camilo
Burbano, Lisseth-Estefania
Guío, Claudia-Marcela
Reyes, Saúl
Cortes, Fabián
Cárdenas-Robledo, Simón
Narváez, Diana M.
Cárdenas, Wilmer
Porras, Alexandra
Lattig, María-Claudia
Groot de Restrepo, Helena
author_sort Toro, Jaime
collection PubMed
description OBJECTIVE: The aim of this study was to determine ancestry informative markers, mitochondrial DNA haplogroups, and the association between HLA-DRB1 alleles and multiple sclerosis (MS) in a group of patients from Bogotá, Colombia. METHODS: In this case-control study, genomic DNA was isolated and purified from blood samples. HLA-DRB1 allele genotyping was done using PCR. Mitochondrial hypervariable region 1 was amplified and haplogroups were determined using HaploGrep software. Genomic ancestry was estimated by genotyping a panel of ancestry informative markers. To test the association of HLA polymorphisms and MS, we ran separate multivariate logistic regression models. Bonferroni correction was used to account for multiple regression tests. RESULTS: A total of 100 patients with MS (mean age 40.4 ± 12 years; 70% females) and 200 healthy controls (mean age 37.6 ± 11 years; 83.5% females) were included in the analysis. Ancestry proportions and haplogroup frequencies did not differ between patients and controls. HLA-DRB1*15 was present in 31% of cases and 13.5% of controls, whereas HLA-DRB1*14 was present in 5% of cases and 15.5% of controls. In the multivariate model, HLA-DRB1*15 was significantly associated with MS (odds ratio [OR] = 3.05, p < 0.001), whereas HLA-DRB1*14 was confirmed as a protective factor in our population (OR = 0.16, p = 0.001). CONCLUSIONS: This study provides evidence indicating that HLA-DRB1*15 allele confers susceptibility to MS and HLA-DRB1*14 allele exerts resistance to MS in a highly admixed population. This latter finding could partially explain the low prevalence of MS in Bogotá, Colombia.
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spelling pubmed-46940722016-01-06 HLA-DRB1*14 is a protective allele for multiple sclerosis in an admixed Colombian population Toro, Jaime Cuellar-Giraldo, David Díaz-Cruz, Camilo Burbano, Lisseth-Estefania Guío, Claudia-Marcela Reyes, Saúl Cortes, Fabián Cárdenas-Robledo, Simón Narváez, Diana M. Cárdenas, Wilmer Porras, Alexandra Lattig, María-Claudia Groot de Restrepo, Helena Neurol Neuroimmunol Neuroinflamm Article OBJECTIVE: The aim of this study was to determine ancestry informative markers, mitochondrial DNA haplogroups, and the association between HLA-DRB1 alleles and multiple sclerosis (MS) in a group of patients from Bogotá, Colombia. METHODS: In this case-control study, genomic DNA was isolated and purified from blood samples. HLA-DRB1 allele genotyping was done using PCR. Mitochondrial hypervariable region 1 was amplified and haplogroups were determined using HaploGrep software. Genomic ancestry was estimated by genotyping a panel of ancestry informative markers. To test the association of HLA polymorphisms and MS, we ran separate multivariate logistic regression models. Bonferroni correction was used to account for multiple regression tests. RESULTS: A total of 100 patients with MS (mean age 40.4 ± 12 years; 70% females) and 200 healthy controls (mean age 37.6 ± 11 years; 83.5% females) were included in the analysis. Ancestry proportions and haplogroup frequencies did not differ between patients and controls. HLA-DRB1*15 was present in 31% of cases and 13.5% of controls, whereas HLA-DRB1*14 was present in 5% of cases and 15.5% of controls. In the multivariate model, HLA-DRB1*15 was significantly associated with MS (odds ratio [OR] = 3.05, p < 0.001), whereas HLA-DRB1*14 was confirmed as a protective factor in our population (OR = 0.16, p = 0.001). CONCLUSIONS: This study provides evidence indicating that HLA-DRB1*15 allele confers susceptibility to MS and HLA-DRB1*14 allele exerts resistance to MS in a highly admixed population. This latter finding could partially explain the low prevalence of MS in Bogotá, Colombia. Lippincott Williams & Wilkins 2015-12-24 /pmc/articles/PMC4694072/ /pubmed/26740965 http://dx.doi.org/10.1212/NXI.0000000000000192 Text en © 2015 American Academy of Neurology This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (http://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits downloading and sharing the work provided it is properly cited. The work cannot be changed in any way or used commercially.
spellingShingle Article
Toro, Jaime
Cuellar-Giraldo, David
Díaz-Cruz, Camilo
Burbano, Lisseth-Estefania
Guío, Claudia-Marcela
Reyes, Saúl
Cortes, Fabián
Cárdenas-Robledo, Simón
Narváez, Diana M.
Cárdenas, Wilmer
Porras, Alexandra
Lattig, María-Claudia
Groot de Restrepo, Helena
HLA-DRB1*14 is a protective allele for multiple sclerosis in an admixed Colombian population
title HLA-DRB1*14 is a protective allele for multiple sclerosis in an admixed Colombian population
title_full HLA-DRB1*14 is a protective allele for multiple sclerosis in an admixed Colombian population
title_fullStr HLA-DRB1*14 is a protective allele for multiple sclerosis in an admixed Colombian population
title_full_unstemmed HLA-DRB1*14 is a protective allele for multiple sclerosis in an admixed Colombian population
title_short HLA-DRB1*14 is a protective allele for multiple sclerosis in an admixed Colombian population
title_sort hla-drb1*14 is a protective allele for multiple sclerosis in an admixed colombian population
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694072/
https://www.ncbi.nlm.nih.gov/pubmed/26740965
http://dx.doi.org/10.1212/NXI.0000000000000192
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