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HLA-DRB1*14 is a protective allele for multiple sclerosis in an admixed Colombian population
OBJECTIVE: The aim of this study was to determine ancestry informative markers, mitochondrial DNA haplogroups, and the association between HLA-DRB1 alleles and multiple sclerosis (MS) in a group of patients from Bogotá, Colombia. METHODS: In this case-control study, genomic DNA was isolated and puri...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Lippincott Williams & Wilkins
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694072/ https://www.ncbi.nlm.nih.gov/pubmed/26740965 http://dx.doi.org/10.1212/NXI.0000000000000192 |
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author | Toro, Jaime Cuellar-Giraldo, David Díaz-Cruz, Camilo Burbano, Lisseth-Estefania Guío, Claudia-Marcela Reyes, Saúl Cortes, Fabián Cárdenas-Robledo, Simón Narváez, Diana M. Cárdenas, Wilmer Porras, Alexandra Lattig, María-Claudia Groot de Restrepo, Helena |
author_facet | Toro, Jaime Cuellar-Giraldo, David Díaz-Cruz, Camilo Burbano, Lisseth-Estefania Guío, Claudia-Marcela Reyes, Saúl Cortes, Fabián Cárdenas-Robledo, Simón Narváez, Diana M. Cárdenas, Wilmer Porras, Alexandra Lattig, María-Claudia Groot de Restrepo, Helena |
author_sort | Toro, Jaime |
collection | PubMed |
description | OBJECTIVE: The aim of this study was to determine ancestry informative markers, mitochondrial DNA haplogroups, and the association between HLA-DRB1 alleles and multiple sclerosis (MS) in a group of patients from Bogotá, Colombia. METHODS: In this case-control study, genomic DNA was isolated and purified from blood samples. HLA-DRB1 allele genotyping was done using PCR. Mitochondrial hypervariable region 1 was amplified and haplogroups were determined using HaploGrep software. Genomic ancestry was estimated by genotyping a panel of ancestry informative markers. To test the association of HLA polymorphisms and MS, we ran separate multivariate logistic regression models. Bonferroni correction was used to account for multiple regression tests. RESULTS: A total of 100 patients with MS (mean age 40.4 ± 12 years; 70% females) and 200 healthy controls (mean age 37.6 ± 11 years; 83.5% females) were included in the analysis. Ancestry proportions and haplogroup frequencies did not differ between patients and controls. HLA-DRB1*15 was present in 31% of cases and 13.5% of controls, whereas HLA-DRB1*14 was present in 5% of cases and 15.5% of controls. In the multivariate model, HLA-DRB1*15 was significantly associated with MS (odds ratio [OR] = 3.05, p < 0.001), whereas HLA-DRB1*14 was confirmed as a protective factor in our population (OR = 0.16, p = 0.001). CONCLUSIONS: This study provides evidence indicating that HLA-DRB1*15 allele confers susceptibility to MS and HLA-DRB1*14 allele exerts resistance to MS in a highly admixed population. This latter finding could partially explain the low prevalence of MS in Bogotá, Colombia. |
format | Online Article Text |
id | pubmed-4694072 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Lippincott Williams & Wilkins |
record_format | MEDLINE/PubMed |
spelling | pubmed-46940722016-01-06 HLA-DRB1*14 is a protective allele for multiple sclerosis in an admixed Colombian population Toro, Jaime Cuellar-Giraldo, David Díaz-Cruz, Camilo Burbano, Lisseth-Estefania Guío, Claudia-Marcela Reyes, Saúl Cortes, Fabián Cárdenas-Robledo, Simón Narváez, Diana M. Cárdenas, Wilmer Porras, Alexandra Lattig, María-Claudia Groot de Restrepo, Helena Neurol Neuroimmunol Neuroinflamm Article OBJECTIVE: The aim of this study was to determine ancestry informative markers, mitochondrial DNA haplogroups, and the association between HLA-DRB1 alleles and multiple sclerosis (MS) in a group of patients from Bogotá, Colombia. METHODS: In this case-control study, genomic DNA was isolated and purified from blood samples. HLA-DRB1 allele genotyping was done using PCR. Mitochondrial hypervariable region 1 was amplified and haplogroups were determined using HaploGrep software. Genomic ancestry was estimated by genotyping a panel of ancestry informative markers. To test the association of HLA polymorphisms and MS, we ran separate multivariate logistic regression models. Bonferroni correction was used to account for multiple regression tests. RESULTS: A total of 100 patients with MS (mean age 40.4 ± 12 years; 70% females) and 200 healthy controls (mean age 37.6 ± 11 years; 83.5% females) were included in the analysis. Ancestry proportions and haplogroup frequencies did not differ between patients and controls. HLA-DRB1*15 was present in 31% of cases and 13.5% of controls, whereas HLA-DRB1*14 was present in 5% of cases and 15.5% of controls. In the multivariate model, HLA-DRB1*15 was significantly associated with MS (odds ratio [OR] = 3.05, p < 0.001), whereas HLA-DRB1*14 was confirmed as a protective factor in our population (OR = 0.16, p = 0.001). CONCLUSIONS: This study provides evidence indicating that HLA-DRB1*15 allele confers susceptibility to MS and HLA-DRB1*14 allele exerts resistance to MS in a highly admixed population. This latter finding could partially explain the low prevalence of MS in Bogotá, Colombia. Lippincott Williams & Wilkins 2015-12-24 /pmc/articles/PMC4694072/ /pubmed/26740965 http://dx.doi.org/10.1212/NXI.0000000000000192 Text en © 2015 American Academy of Neurology This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (http://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits downloading and sharing the work provided it is properly cited. The work cannot be changed in any way or used commercially. |
spellingShingle | Article Toro, Jaime Cuellar-Giraldo, David Díaz-Cruz, Camilo Burbano, Lisseth-Estefania Guío, Claudia-Marcela Reyes, Saúl Cortes, Fabián Cárdenas-Robledo, Simón Narváez, Diana M. Cárdenas, Wilmer Porras, Alexandra Lattig, María-Claudia Groot de Restrepo, Helena HLA-DRB1*14 is a protective allele for multiple sclerosis in an admixed Colombian population |
title | HLA-DRB1*14 is a protective allele for multiple sclerosis in an admixed Colombian population |
title_full | HLA-DRB1*14 is a protective allele for multiple sclerosis in an admixed Colombian population |
title_fullStr | HLA-DRB1*14 is a protective allele for multiple sclerosis in an admixed Colombian population |
title_full_unstemmed | HLA-DRB1*14 is a protective allele for multiple sclerosis in an admixed Colombian population |
title_short | HLA-DRB1*14 is a protective allele for multiple sclerosis in an admixed Colombian population |
title_sort | hla-drb1*14 is a protective allele for multiple sclerosis in an admixed colombian population |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694072/ https://www.ncbi.nlm.nih.gov/pubmed/26740965 http://dx.doi.org/10.1212/NXI.0000000000000192 |
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