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Methicillin‐resistant Staphylococcus pseudintermedius (MRSP) from healthy dogs in Norway – occurrence, genotypes and comparison to clinical MRSP

The aim of the study was to investigate the occurrence of methicillin‐resistant Staphylococcus pseudintermedius (MRSP) in healthy dogs and further to determine genetic relatedness between carrier isolates and clinical MRSP from dogs in Norway. A total of 189 healthy dogs visiting ten veterinary clin...

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Autores principales: Kjellman, Ellen Eide, Slettemeås, Jannice Schau, Small, Harald, Sunde, Marianne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694142/
https://www.ncbi.nlm.nih.gov/pubmed/26423808
http://dx.doi.org/10.1002/mbo3.258
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author Kjellman, Ellen Eide
Slettemeås, Jannice Schau
Small, Harald
Sunde, Marianne
author_facet Kjellman, Ellen Eide
Slettemeås, Jannice Schau
Small, Harald
Sunde, Marianne
author_sort Kjellman, Ellen Eide
collection PubMed
description The aim of the study was to investigate the occurrence of methicillin‐resistant Staphylococcus pseudintermedius (MRSP) in healthy dogs and further to determine genetic relatedness between carrier isolates and clinical MRSP from dogs in Norway. A total of 189 healthy dogs visiting ten veterinary clinics were screened for MRSP during the period February to April 2013. Carrier isolates were susceptibility tested with disk diffusion and genotyped using multilocus sequence typing (MLST) and pulsed‐field gel electrophoresis (PFGE). Forty‐nine clinical MRSP were characterized for comparison. These isolates were collected from July 2008 to April 2013 and represent all MRSP index isolates from each MRSP‐positive dog detected in Norway until April 2013. Geographical distribution of all MRSP cases was investigated using the ArcGIS 9.3 Software. MRSP was detected from five (2.6%) healthy dogs, sampled at three different clinics. The isolates grouped into three sequence types (STs): ST252 (two isolates), ST71 (two isolates) and ST306 (one isolate). MRSP from dogs sampled at the same animal clinic belonged to the same ST and produced identical PFGE pattern. The 49 clinical MRSP grouped into 15 STs; ST258 (n = 17), ST71 (n = 10), and ST305 (n = 4) were the most prevalent. The MRSP carrier isolates were genetically related to MRSP variants from dogs with infections as ST306 (from a carrier) is related to ST258. MRSP ST252, found in two carriers, was also present among the clinical MRSP isolates. Altogether the MRSP isolates were genetically diverse and MRSP of other lineages than ST71 continues to disseminate in Norway. Susceptibility testing showed that MRSP isolates of the ST71 lineage were the most multiresistant. Our study showed that MRSP could be detected in healthy dogs without infections and with no recent history of antimicrobial therapy stressing the need for future monitoring, infection control and prudent use of antimicrobial agents.
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spelling pubmed-46941422016-01-06 Methicillin‐resistant Staphylococcus pseudintermedius (MRSP) from healthy dogs in Norway – occurrence, genotypes and comparison to clinical MRSP Kjellman, Ellen Eide Slettemeås, Jannice Schau Small, Harald Sunde, Marianne Microbiologyopen Original Research The aim of the study was to investigate the occurrence of methicillin‐resistant Staphylococcus pseudintermedius (MRSP) in healthy dogs and further to determine genetic relatedness between carrier isolates and clinical MRSP from dogs in Norway. A total of 189 healthy dogs visiting ten veterinary clinics were screened for MRSP during the period February to April 2013. Carrier isolates were susceptibility tested with disk diffusion and genotyped using multilocus sequence typing (MLST) and pulsed‐field gel electrophoresis (PFGE). Forty‐nine clinical MRSP were characterized for comparison. These isolates were collected from July 2008 to April 2013 and represent all MRSP index isolates from each MRSP‐positive dog detected in Norway until April 2013. Geographical distribution of all MRSP cases was investigated using the ArcGIS 9.3 Software. MRSP was detected from five (2.6%) healthy dogs, sampled at three different clinics. The isolates grouped into three sequence types (STs): ST252 (two isolates), ST71 (two isolates) and ST306 (one isolate). MRSP from dogs sampled at the same animal clinic belonged to the same ST and produced identical PFGE pattern. The 49 clinical MRSP grouped into 15 STs; ST258 (n = 17), ST71 (n = 10), and ST305 (n = 4) were the most prevalent. The MRSP carrier isolates were genetically related to MRSP variants from dogs with infections as ST306 (from a carrier) is related to ST258. MRSP ST252, found in two carriers, was also present among the clinical MRSP isolates. Altogether the MRSP isolates were genetically diverse and MRSP of other lineages than ST71 continues to disseminate in Norway. Susceptibility testing showed that MRSP isolates of the ST71 lineage were the most multiresistant. Our study showed that MRSP could be detected in healthy dogs without infections and with no recent history of antimicrobial therapy stressing the need for future monitoring, infection control and prudent use of antimicrobial agents. John Wiley and Sons Inc. 2015-10-01 /pmc/articles/PMC4694142/ /pubmed/26423808 http://dx.doi.org/10.1002/mbo3.258 Text en © 2015 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Kjellman, Ellen Eide
Slettemeås, Jannice Schau
Small, Harald
Sunde, Marianne
Methicillin‐resistant Staphylococcus pseudintermedius (MRSP) from healthy dogs in Norway – occurrence, genotypes and comparison to clinical MRSP
title Methicillin‐resistant Staphylococcus pseudintermedius (MRSP) from healthy dogs in Norway – occurrence, genotypes and comparison to clinical MRSP
title_full Methicillin‐resistant Staphylococcus pseudintermedius (MRSP) from healthy dogs in Norway – occurrence, genotypes and comparison to clinical MRSP
title_fullStr Methicillin‐resistant Staphylococcus pseudintermedius (MRSP) from healthy dogs in Norway – occurrence, genotypes and comparison to clinical MRSP
title_full_unstemmed Methicillin‐resistant Staphylococcus pseudintermedius (MRSP) from healthy dogs in Norway – occurrence, genotypes and comparison to clinical MRSP
title_short Methicillin‐resistant Staphylococcus pseudintermedius (MRSP) from healthy dogs in Norway – occurrence, genotypes and comparison to clinical MRSP
title_sort methicillin‐resistant staphylococcus pseudintermedius (mrsp) from healthy dogs in norway – occurrence, genotypes and comparison to clinical mrsp
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694142/
https://www.ncbi.nlm.nih.gov/pubmed/26423808
http://dx.doi.org/10.1002/mbo3.258
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