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Single-filament kinetic studies provide novel insights into regulation of actin-based motility

Polarized assembly of actin filaments forms the basis of actin-based motility and is regulated both spatially and temporally. Cells use a variety of mechanisms by which intrinsically slower processes are accelerated, and faster ones decelerated, to match rates observed in vivo. Here we discuss how k...

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Detalles Bibliográficos
Autores principales: Shekhar, Shashank, Carlier, Marie-France
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694749/
https://www.ncbi.nlm.nih.gov/pubmed/26715420
http://dx.doi.org/10.1091/mbc.E15-06-0352
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author Shekhar, Shashank
Carlier, Marie-France
author_facet Shekhar, Shashank
Carlier, Marie-France
author_sort Shekhar, Shashank
collection PubMed
description Polarized assembly of actin filaments forms the basis of actin-based motility and is regulated both spatially and temporally. Cells use a variety of mechanisms by which intrinsically slower processes are accelerated, and faster ones decelerated, to match rates observed in vivo. Here we discuss how kinetic studies of individual reactions and cycles that drive actin remodeling have provided a mechanistic and quantitative understanding of such processes. We specifically consider key barbed-end regulators such as capping protein and formins as illustrative examples. We compare and contrast different kinetic approaches, such as the traditional pyrene-polymerization bulk assays, as well as more recently developed single-filament and single-molecule imaging approaches. Recent development of novel biophysical methods for sensing and applying forces will in future allow us to address the very important relationship between mechanical stimulus and kinetics of actin-based motility.
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spelling pubmed-46947492016-03-16 Single-filament kinetic studies provide novel insights into regulation of actin-based motility Shekhar, Shashank Carlier, Marie-France Mol Biol Cell Perspectives Polarized assembly of actin filaments forms the basis of actin-based motility and is regulated both spatially and temporally. Cells use a variety of mechanisms by which intrinsically slower processes are accelerated, and faster ones decelerated, to match rates observed in vivo. Here we discuss how kinetic studies of individual reactions and cycles that drive actin remodeling have provided a mechanistic and quantitative understanding of such processes. We specifically consider key barbed-end regulators such as capping protein and formins as illustrative examples. We compare and contrast different kinetic approaches, such as the traditional pyrene-polymerization bulk assays, as well as more recently developed single-filament and single-molecule imaging approaches. Recent development of novel biophysical methods for sensing and applying forces will in future allow us to address the very important relationship between mechanical stimulus and kinetics of actin-based motility. The American Society for Cell Biology 2016-01-01 /pmc/articles/PMC4694749/ /pubmed/26715420 http://dx.doi.org/10.1091/mbc.E15-06-0352 Text en © 2016 Shekhar and Carlier. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Perspectives
Shekhar, Shashank
Carlier, Marie-France
Single-filament kinetic studies provide novel insights into regulation of actin-based motility
title Single-filament kinetic studies provide novel insights into regulation of actin-based motility
title_full Single-filament kinetic studies provide novel insights into regulation of actin-based motility
title_fullStr Single-filament kinetic studies provide novel insights into regulation of actin-based motility
title_full_unstemmed Single-filament kinetic studies provide novel insights into regulation of actin-based motility
title_short Single-filament kinetic studies provide novel insights into regulation of actin-based motility
title_sort single-filament kinetic studies provide novel insights into regulation of actin-based motility
topic Perspectives
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694749/
https://www.ncbi.nlm.nih.gov/pubmed/26715420
http://dx.doi.org/10.1091/mbc.E15-06-0352
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