Cargando…
Single-filament kinetic studies provide novel insights into regulation of actin-based motility
Polarized assembly of actin filaments forms the basis of actin-based motility and is regulated both spatially and temporally. Cells use a variety of mechanisms by which intrinsically slower processes are accelerated, and faster ones decelerated, to match rates observed in vivo. Here we discuss how k...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694749/ https://www.ncbi.nlm.nih.gov/pubmed/26715420 http://dx.doi.org/10.1091/mbc.E15-06-0352 |
_version_ | 1782407513628475392 |
---|---|
author | Shekhar, Shashank Carlier, Marie-France |
author_facet | Shekhar, Shashank Carlier, Marie-France |
author_sort | Shekhar, Shashank |
collection | PubMed |
description | Polarized assembly of actin filaments forms the basis of actin-based motility and is regulated both spatially and temporally. Cells use a variety of mechanisms by which intrinsically slower processes are accelerated, and faster ones decelerated, to match rates observed in vivo. Here we discuss how kinetic studies of individual reactions and cycles that drive actin remodeling have provided a mechanistic and quantitative understanding of such processes. We specifically consider key barbed-end regulators such as capping protein and formins as illustrative examples. We compare and contrast different kinetic approaches, such as the traditional pyrene-polymerization bulk assays, as well as more recently developed single-filament and single-molecule imaging approaches. Recent development of novel biophysical methods for sensing and applying forces will in future allow us to address the very important relationship between mechanical stimulus and kinetics of actin-based motility. |
format | Online Article Text |
id | pubmed-4694749 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-46947492016-03-16 Single-filament kinetic studies provide novel insights into regulation of actin-based motility Shekhar, Shashank Carlier, Marie-France Mol Biol Cell Perspectives Polarized assembly of actin filaments forms the basis of actin-based motility and is regulated both spatially and temporally. Cells use a variety of mechanisms by which intrinsically slower processes are accelerated, and faster ones decelerated, to match rates observed in vivo. Here we discuss how kinetic studies of individual reactions and cycles that drive actin remodeling have provided a mechanistic and quantitative understanding of such processes. We specifically consider key barbed-end regulators such as capping protein and formins as illustrative examples. We compare and contrast different kinetic approaches, such as the traditional pyrene-polymerization bulk assays, as well as more recently developed single-filament and single-molecule imaging approaches. Recent development of novel biophysical methods for sensing and applying forces will in future allow us to address the very important relationship between mechanical stimulus and kinetics of actin-based motility. The American Society for Cell Biology 2016-01-01 /pmc/articles/PMC4694749/ /pubmed/26715420 http://dx.doi.org/10.1091/mbc.E15-06-0352 Text en © 2016 Shekhar and Carlier. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. |
spellingShingle | Perspectives Shekhar, Shashank Carlier, Marie-France Single-filament kinetic studies provide novel insights into regulation of actin-based motility |
title | Single-filament kinetic studies provide novel insights into regulation of actin-based motility |
title_full | Single-filament kinetic studies provide novel insights into regulation of actin-based motility |
title_fullStr | Single-filament kinetic studies provide novel insights into regulation of actin-based motility |
title_full_unstemmed | Single-filament kinetic studies provide novel insights into regulation of actin-based motility |
title_short | Single-filament kinetic studies provide novel insights into regulation of actin-based motility |
title_sort | single-filament kinetic studies provide novel insights into regulation of actin-based motility |
topic | Perspectives |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694749/ https://www.ncbi.nlm.nih.gov/pubmed/26715420 http://dx.doi.org/10.1091/mbc.E15-06-0352 |
work_keys_str_mv | AT shekharshashank singlefilamentkineticstudiesprovidenovelinsightsintoregulationofactinbasedmotility AT carliermariefrance singlefilamentkineticstudiesprovidenovelinsightsintoregulationofactinbasedmotility |