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Prokineticin 2 (PROK2) is an important factor for angiogenesis in colorectal cancer
The Prokineticin 2 (PROK2) is correlated with indispensable in maintaining the homeostasis of healthy human tissues. Herein, we examined the role of PROK2 in human colorectal cancer. After total RNA extraction from 6 colorectal cancer cell lines, we examined the expression of PROK2 mRNA. For investi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694898/ https://www.ncbi.nlm.nih.gov/pubmed/26317645 |
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author | Kurebayashi, Hidetaka Goi, Takanori Shimada, Michiaki Tagai, Noriyuki Naruse, Takayuki Nakazawa, Toshiyuki Kimura, Youhei Hirono, Yasuo Yamaguchi, Akio |
author_facet | Kurebayashi, Hidetaka Goi, Takanori Shimada, Michiaki Tagai, Noriyuki Naruse, Takayuki Nakazawa, Toshiyuki Kimura, Youhei Hirono, Yasuo Yamaguchi, Akio |
author_sort | Kurebayashi, Hidetaka |
collection | PubMed |
description | The Prokineticin 2 (PROK2) is correlated with indispensable in maintaining the homeostasis of healthy human tissues. Herein, we examined the role of PROK2 in human colorectal cancer. After total RNA extraction from 6 colorectal cancer cell lines, we examined the expression of PROK2 mRNA. For investigating angiogenesis and tumor growth in mice, the PROK2 gene was transfected into colorectal cancer cell lines having low PROK2 mRNA expression. In addition, small interfering RNA (siRNA) was transfected into colorectal cancer cell lines having high PROK2 mRNA expression for investigation of angiogenesis and tumor growth in mice. From 6 colorectal cancer cell lines studied, PROK2 mRNA expression was increased in 3 cell lines. When the PROK2 gene was transfected into the colorectal cancer cell line with low PROK2 mRNA expression, angiogenesis and tumor growth in mice increased significantly compared to the cell line with the control vector. When PROK2 siRNA was transfected into colorectal cancer cell lines with high PROK2 mRNA expression, angiogenesis and tumor growth in mice were suppressed significantly compared to the cell line with siRNA (control). This is the first report of the association of PROK2 as an angiogenic growth factor in colorectal cancer. |
format | Online Article Text |
id | pubmed-4694898 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-46948982016-01-20 Prokineticin 2 (PROK2) is an important factor for angiogenesis in colorectal cancer Kurebayashi, Hidetaka Goi, Takanori Shimada, Michiaki Tagai, Noriyuki Naruse, Takayuki Nakazawa, Toshiyuki Kimura, Youhei Hirono, Yasuo Yamaguchi, Akio Oncotarget Research Paper The Prokineticin 2 (PROK2) is correlated with indispensable in maintaining the homeostasis of healthy human tissues. Herein, we examined the role of PROK2 in human colorectal cancer. After total RNA extraction from 6 colorectal cancer cell lines, we examined the expression of PROK2 mRNA. For investigating angiogenesis and tumor growth in mice, the PROK2 gene was transfected into colorectal cancer cell lines having low PROK2 mRNA expression. In addition, small interfering RNA (siRNA) was transfected into colorectal cancer cell lines having high PROK2 mRNA expression for investigation of angiogenesis and tumor growth in mice. From 6 colorectal cancer cell lines studied, PROK2 mRNA expression was increased in 3 cell lines. When the PROK2 gene was transfected into the colorectal cancer cell line with low PROK2 mRNA expression, angiogenesis and tumor growth in mice increased significantly compared to the cell line with the control vector. When PROK2 siRNA was transfected into colorectal cancer cell lines with high PROK2 mRNA expression, angiogenesis and tumor growth in mice were suppressed significantly compared to the cell line with siRNA (control). This is the first report of the association of PROK2 as an angiogenic growth factor in colorectal cancer. Impact Journals LLC 2015-06-30 /pmc/articles/PMC4694898/ /pubmed/26317645 Text en Copyright: © 2015 Kurebayashi et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Kurebayashi, Hidetaka Goi, Takanori Shimada, Michiaki Tagai, Noriyuki Naruse, Takayuki Nakazawa, Toshiyuki Kimura, Youhei Hirono, Yasuo Yamaguchi, Akio Prokineticin 2 (PROK2) is an important factor for angiogenesis in colorectal cancer |
title | Prokineticin 2 (PROK2) is an important factor for angiogenesis in colorectal cancer |
title_full | Prokineticin 2 (PROK2) is an important factor for angiogenesis in colorectal cancer |
title_fullStr | Prokineticin 2 (PROK2) is an important factor for angiogenesis in colorectal cancer |
title_full_unstemmed | Prokineticin 2 (PROK2) is an important factor for angiogenesis in colorectal cancer |
title_short | Prokineticin 2 (PROK2) is an important factor for angiogenesis in colorectal cancer |
title_sort | prokineticin 2 (prok2) is an important factor for angiogenesis in colorectal cancer |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694898/ https://www.ncbi.nlm.nih.gov/pubmed/26317645 |
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