Cargando…

Prokineticin 2 (PROK2) is an important factor for angiogenesis in colorectal cancer

The Prokineticin 2 (PROK2) is correlated with indispensable in maintaining the homeostasis of healthy human tissues. Herein, we examined the role of PROK2 in human colorectal cancer. After total RNA extraction from 6 colorectal cancer cell lines, we examined the expression of PROK2 mRNA. For investi...

Descripción completa

Detalles Bibliográficos
Autores principales: Kurebayashi, Hidetaka, Goi, Takanori, Shimada, Michiaki, Tagai, Noriyuki, Naruse, Takayuki, Nakazawa, Toshiyuki, Kimura, Youhei, Hirono, Yasuo, Yamaguchi, Akio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694898/
https://www.ncbi.nlm.nih.gov/pubmed/26317645
_version_ 1782407548412887040
author Kurebayashi, Hidetaka
Goi, Takanori
Shimada, Michiaki
Tagai, Noriyuki
Naruse, Takayuki
Nakazawa, Toshiyuki
Kimura, Youhei
Hirono, Yasuo
Yamaguchi, Akio
author_facet Kurebayashi, Hidetaka
Goi, Takanori
Shimada, Michiaki
Tagai, Noriyuki
Naruse, Takayuki
Nakazawa, Toshiyuki
Kimura, Youhei
Hirono, Yasuo
Yamaguchi, Akio
author_sort Kurebayashi, Hidetaka
collection PubMed
description The Prokineticin 2 (PROK2) is correlated with indispensable in maintaining the homeostasis of healthy human tissues. Herein, we examined the role of PROK2 in human colorectal cancer. After total RNA extraction from 6 colorectal cancer cell lines, we examined the expression of PROK2 mRNA. For investigating angiogenesis and tumor growth in mice, the PROK2 gene was transfected into colorectal cancer cell lines having low PROK2 mRNA expression. In addition, small interfering RNA (siRNA) was transfected into colorectal cancer cell lines having high PROK2 mRNA expression for investigation of angiogenesis and tumor growth in mice. From 6 colorectal cancer cell lines studied, PROK2 mRNA expression was increased in 3 cell lines. When the PROK2 gene was transfected into the colorectal cancer cell line with low PROK2 mRNA expression, angiogenesis and tumor growth in mice increased significantly compared to the cell line with the control vector. When PROK2 siRNA was transfected into colorectal cancer cell lines with high PROK2 mRNA expression, angiogenesis and tumor growth in mice were suppressed significantly compared to the cell line with siRNA (control). This is the first report of the association of PROK2 as an angiogenic growth factor in colorectal cancer.
format Online
Article
Text
id pubmed-4694898
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Impact Journals LLC
record_format MEDLINE/PubMed
spelling pubmed-46948982016-01-20 Prokineticin 2 (PROK2) is an important factor for angiogenesis in colorectal cancer Kurebayashi, Hidetaka Goi, Takanori Shimada, Michiaki Tagai, Noriyuki Naruse, Takayuki Nakazawa, Toshiyuki Kimura, Youhei Hirono, Yasuo Yamaguchi, Akio Oncotarget Research Paper The Prokineticin 2 (PROK2) is correlated with indispensable in maintaining the homeostasis of healthy human tissues. Herein, we examined the role of PROK2 in human colorectal cancer. After total RNA extraction from 6 colorectal cancer cell lines, we examined the expression of PROK2 mRNA. For investigating angiogenesis and tumor growth in mice, the PROK2 gene was transfected into colorectal cancer cell lines having low PROK2 mRNA expression. In addition, small interfering RNA (siRNA) was transfected into colorectal cancer cell lines having high PROK2 mRNA expression for investigation of angiogenesis and tumor growth in mice. From 6 colorectal cancer cell lines studied, PROK2 mRNA expression was increased in 3 cell lines. When the PROK2 gene was transfected into the colorectal cancer cell line with low PROK2 mRNA expression, angiogenesis and tumor growth in mice increased significantly compared to the cell line with the control vector. When PROK2 siRNA was transfected into colorectal cancer cell lines with high PROK2 mRNA expression, angiogenesis and tumor growth in mice were suppressed significantly compared to the cell line with siRNA (control). This is the first report of the association of PROK2 as an angiogenic growth factor in colorectal cancer. Impact Journals LLC 2015-06-30 /pmc/articles/PMC4694898/ /pubmed/26317645 Text en Copyright: © 2015 Kurebayashi et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Kurebayashi, Hidetaka
Goi, Takanori
Shimada, Michiaki
Tagai, Noriyuki
Naruse, Takayuki
Nakazawa, Toshiyuki
Kimura, Youhei
Hirono, Yasuo
Yamaguchi, Akio
Prokineticin 2 (PROK2) is an important factor for angiogenesis in colorectal cancer
title Prokineticin 2 (PROK2) is an important factor for angiogenesis in colorectal cancer
title_full Prokineticin 2 (PROK2) is an important factor for angiogenesis in colorectal cancer
title_fullStr Prokineticin 2 (PROK2) is an important factor for angiogenesis in colorectal cancer
title_full_unstemmed Prokineticin 2 (PROK2) is an important factor for angiogenesis in colorectal cancer
title_short Prokineticin 2 (PROK2) is an important factor for angiogenesis in colorectal cancer
title_sort prokineticin 2 (prok2) is an important factor for angiogenesis in colorectal cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694898/
https://www.ncbi.nlm.nih.gov/pubmed/26317645
work_keys_str_mv AT kurebayashihidetaka prokineticin2prok2isanimportantfactorforangiogenesisincolorectalcancer
AT goitakanori prokineticin2prok2isanimportantfactorforangiogenesisincolorectalcancer
AT shimadamichiaki prokineticin2prok2isanimportantfactorforangiogenesisincolorectalcancer
AT tagainoriyuki prokineticin2prok2isanimportantfactorforangiogenesisincolorectalcancer
AT narusetakayuki prokineticin2prok2isanimportantfactorforangiogenesisincolorectalcancer
AT nakazawatoshiyuki prokineticin2prok2isanimportantfactorforangiogenesisincolorectalcancer
AT kimurayouhei prokineticin2prok2isanimportantfactorforangiogenesisincolorectalcancer
AT hironoyasuo prokineticin2prok2isanimportantfactorforangiogenesisincolorectalcancer
AT yamaguchiakio prokineticin2prok2isanimportantfactorforangiogenesisincolorectalcancer