Cargando…

Stromal expression of ALDH1 in human breast carcinomas indicates reduced tumor progression

Interactions between cancer cells and microenvironment are emerging issue in tumor progression. Aldehyde dehydrogenase 1 (ALDH1) is a recognized cancer stem cell marker but little is known about its role in intratumoral stroma. Therefore, we focused on ALDH1 expression in tumor-associated stroma of...

Descripción completa

Detalles Bibliográficos
Autores principales: Bednarz-Knoll, Natalia, Nastały, Paulina, Żaczek, Anna, Stoupiec, Małgorzata, Riethdorf, Sabine, Wikman, Harriet, Müller, Volkmar, Skokowski, Jarosław, Szade, Jolanta, Sejda, Aleksandra, Wełnicka-Jaśkiewicz, Marzena, Pantel, Klaus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694953/
https://www.ncbi.nlm.nih.gov/pubmed/26305673
_version_ 1782407561495969792
author Bednarz-Knoll, Natalia
Nastały, Paulina
Żaczek, Anna
Stoupiec, Małgorzata
Riethdorf, Sabine
Wikman, Harriet
Müller, Volkmar
Skokowski, Jarosław
Szade, Jolanta
Sejda, Aleksandra
Wełnicka-Jaśkiewicz, Marzena
Pantel, Klaus
author_facet Bednarz-Knoll, Natalia
Nastały, Paulina
Żaczek, Anna
Stoupiec, Małgorzata
Riethdorf, Sabine
Wikman, Harriet
Müller, Volkmar
Skokowski, Jarosław
Szade, Jolanta
Sejda, Aleksandra
Wełnicka-Jaśkiewicz, Marzena
Pantel, Klaus
author_sort Bednarz-Knoll, Natalia
collection PubMed
description Interactions between cancer cells and microenvironment are emerging issue in tumor progression. Aldehyde dehydrogenase 1 (ALDH1) is a recognized cancer stem cell marker but little is known about its role in intratumoral stroma. Therefore, we focused on ALDH1 expression in tumor-associated stroma of breast carcinomas (BrCa). Stromal and tumoral ALDH1 expression was evaluated immunohistochemically in BrCa and their lymph node metastases (LNMs), and related to clinico-pathological characteristics, patients’ outcome, presence of CD68, HLADR, retinoic acid (RA) in stroma, and selected proteins in tumor cells. ALDH1(+) stromal cells were detected in 53% of 374 BrCa and 61% of 102 LNMs. ALDH1(+) stroma in primary tumor correlated to longer disease-free (p = 0.030), metastasis-free (p = 0.024), and overall survival (p = 0.043) having an independent prognostic impact on DFS (multivariate analysis, p = 0.047). It was associated with concomitant presence of HLA-DR(+) stromal cells and RA in tumor cells (both p < 0.001), and inversely associated with vimentin expression in tumor cells (p = 0.036). ALDH1(+) stroma in LNMs correlated inversely to presence of disseminated tumor cells in patients’ bone marrow (p = 0.014) and was independent prognosticator of shorter DFS and MFS (multivariate analysis, p = 0.004 and p = 0.002, respectively). In conclusion, ALDH1 expression in tumor-associated stromal cells indicates reduced BrCa progression, possibly via RA secretion.
format Online
Article
Text
id pubmed-4694953
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Impact Journals LLC
record_format MEDLINE/PubMed
spelling pubmed-46949532016-01-20 Stromal expression of ALDH1 in human breast carcinomas indicates reduced tumor progression Bednarz-Knoll, Natalia Nastały, Paulina Żaczek, Anna Stoupiec, Małgorzata Riethdorf, Sabine Wikman, Harriet Müller, Volkmar Skokowski, Jarosław Szade, Jolanta Sejda, Aleksandra Wełnicka-Jaśkiewicz, Marzena Pantel, Klaus Oncotarget Research Paper: Pathology Interactions between cancer cells and microenvironment are emerging issue in tumor progression. Aldehyde dehydrogenase 1 (ALDH1) is a recognized cancer stem cell marker but little is known about its role in intratumoral stroma. Therefore, we focused on ALDH1 expression in tumor-associated stroma of breast carcinomas (BrCa). Stromal and tumoral ALDH1 expression was evaluated immunohistochemically in BrCa and their lymph node metastases (LNMs), and related to clinico-pathological characteristics, patients’ outcome, presence of CD68, HLADR, retinoic acid (RA) in stroma, and selected proteins in tumor cells. ALDH1(+) stromal cells were detected in 53% of 374 BrCa and 61% of 102 LNMs. ALDH1(+) stroma in primary tumor correlated to longer disease-free (p = 0.030), metastasis-free (p = 0.024), and overall survival (p = 0.043) having an independent prognostic impact on DFS (multivariate analysis, p = 0.047). It was associated with concomitant presence of HLA-DR(+) stromal cells and RA in tumor cells (both p < 0.001), and inversely associated with vimentin expression in tumor cells (p = 0.036). ALDH1(+) stroma in LNMs correlated inversely to presence of disseminated tumor cells in patients’ bone marrow (p = 0.014) and was independent prognosticator of shorter DFS and MFS (multivariate analysis, p = 0.004 and p = 0.002, respectively). In conclusion, ALDH1 expression in tumor-associated stromal cells indicates reduced BrCa progression, possibly via RA secretion. Impact Journals LLC 2015-07-20 /pmc/articles/PMC4694953/ /pubmed/26305673 Text en Copyright: © 2015 Bednarz-Knoll et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper: Pathology
Bednarz-Knoll, Natalia
Nastały, Paulina
Żaczek, Anna
Stoupiec, Małgorzata
Riethdorf, Sabine
Wikman, Harriet
Müller, Volkmar
Skokowski, Jarosław
Szade, Jolanta
Sejda, Aleksandra
Wełnicka-Jaśkiewicz, Marzena
Pantel, Klaus
Stromal expression of ALDH1 in human breast carcinomas indicates reduced tumor progression
title Stromal expression of ALDH1 in human breast carcinomas indicates reduced tumor progression
title_full Stromal expression of ALDH1 in human breast carcinomas indicates reduced tumor progression
title_fullStr Stromal expression of ALDH1 in human breast carcinomas indicates reduced tumor progression
title_full_unstemmed Stromal expression of ALDH1 in human breast carcinomas indicates reduced tumor progression
title_short Stromal expression of ALDH1 in human breast carcinomas indicates reduced tumor progression
title_sort stromal expression of aldh1 in human breast carcinomas indicates reduced tumor progression
topic Research Paper: Pathology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694953/
https://www.ncbi.nlm.nih.gov/pubmed/26305673
work_keys_str_mv AT bednarzknollnatalia stromalexpressionofaldh1inhumanbreastcarcinomasindicatesreducedtumorprogression
AT nastałypaulina stromalexpressionofaldh1inhumanbreastcarcinomasindicatesreducedtumorprogression
AT zaczekanna stromalexpressionofaldh1inhumanbreastcarcinomasindicatesreducedtumorprogression
AT stoupiecmałgorzata stromalexpressionofaldh1inhumanbreastcarcinomasindicatesreducedtumorprogression
AT riethdorfsabine stromalexpressionofaldh1inhumanbreastcarcinomasindicatesreducedtumorprogression
AT wikmanharriet stromalexpressionofaldh1inhumanbreastcarcinomasindicatesreducedtumorprogression
AT mullervolkmar stromalexpressionofaldh1inhumanbreastcarcinomasindicatesreducedtumorprogression
AT skokowskijarosław stromalexpressionofaldh1inhumanbreastcarcinomasindicatesreducedtumorprogression
AT szadejolanta stromalexpressionofaldh1inhumanbreastcarcinomasindicatesreducedtumorprogression
AT sejdaaleksandra stromalexpressionofaldh1inhumanbreastcarcinomasindicatesreducedtumorprogression
AT wełnickajaskiewiczmarzena stromalexpressionofaldh1inhumanbreastcarcinomasindicatesreducedtumorprogression
AT pantelklaus stromalexpressionofaldh1inhumanbreastcarcinomasindicatesreducedtumorprogression