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Protein tyrosine phosphatase SHP2 promotes invadopodia formation through suppression of Rho signaling
Invadopodia are actin-enriched membrane protrusions that are important for extracellular matrix degradation and invasive cell motility. Src homolog domain-containing phosphatase 2 (SHP2), a non-receptor protein tyrosine phosphatase, has been shown to play an important role in promoting cancer metast...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4695156/ https://www.ncbi.nlm.nih.gov/pubmed/26204488 |
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author | Tsai, Wan-Chen Chen, Chien-Lin Chen, Hong-Chen |
author_facet | Tsai, Wan-Chen Chen, Chien-Lin Chen, Hong-Chen |
author_sort | Tsai, Wan-Chen |
collection | PubMed |
description | Invadopodia are actin-enriched membrane protrusions that are important for extracellular matrix degradation and invasive cell motility. Src homolog domain-containing phosphatase 2 (SHP2), a non-receptor protein tyrosine phosphatase, has been shown to play an important role in promoting cancer metastasis, but the underlying mechanism is unclear. In this study, we found that depletion of SHP2 by short-hairpin RNA suppressed invadopodia formation in several cancer cell lines, particularly in the SAS head and neck squamous cell line. In contrast, overexpression of SHP2 promoted invadopodia formation in the CAL27 head and neck squamous cell line, which expresses low levels of endogenous SHP2. The depletion of SHP2 in SAS cells significantly decreased their invasive motility. The suppression of invadopodia formation by SHP2 depletion was restored by the Clostridium botulinum C3 exoenzyme (a Rho GTPase inhibitor) or Y27632 (a specific inhibitor for Rho-associated kinase). Together, our results suggest that SHP2 may promote invadopodia formation through inhibition of Rho signaling in cancer cells. |
format | Online Article Text |
id | pubmed-4695156 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-46951562016-01-26 Protein tyrosine phosphatase SHP2 promotes invadopodia formation through suppression of Rho signaling Tsai, Wan-Chen Chen, Chien-Lin Chen, Hong-Chen Oncotarget Research Paper Invadopodia are actin-enriched membrane protrusions that are important for extracellular matrix degradation and invasive cell motility. Src homolog domain-containing phosphatase 2 (SHP2), a non-receptor protein tyrosine phosphatase, has been shown to play an important role in promoting cancer metastasis, but the underlying mechanism is unclear. In this study, we found that depletion of SHP2 by short-hairpin RNA suppressed invadopodia formation in several cancer cell lines, particularly in the SAS head and neck squamous cell line. In contrast, overexpression of SHP2 promoted invadopodia formation in the CAL27 head and neck squamous cell line, which expresses low levels of endogenous SHP2. The depletion of SHP2 in SAS cells significantly decreased their invasive motility. The suppression of invadopodia formation by SHP2 depletion was restored by the Clostridium botulinum C3 exoenzyme (a Rho GTPase inhibitor) or Y27632 (a specific inhibitor for Rho-associated kinase). Together, our results suggest that SHP2 may promote invadopodia formation through inhibition of Rho signaling in cancer cells. Impact Journals LLC 2015-06-19 /pmc/articles/PMC4695156/ /pubmed/26204488 Text en Copyright: © 2015 Tsai et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Tsai, Wan-Chen Chen, Chien-Lin Chen, Hong-Chen Protein tyrosine phosphatase SHP2 promotes invadopodia formation through suppression of Rho signaling |
title | Protein tyrosine phosphatase SHP2 promotes invadopodia formation through suppression of Rho signaling |
title_full | Protein tyrosine phosphatase SHP2 promotes invadopodia formation through suppression of Rho signaling |
title_fullStr | Protein tyrosine phosphatase SHP2 promotes invadopodia formation through suppression of Rho signaling |
title_full_unstemmed | Protein tyrosine phosphatase SHP2 promotes invadopodia formation through suppression of Rho signaling |
title_short | Protein tyrosine phosphatase SHP2 promotes invadopodia formation through suppression of Rho signaling |
title_sort | protein tyrosine phosphatase shp2 promotes invadopodia formation through suppression of rho signaling |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4695156/ https://www.ncbi.nlm.nih.gov/pubmed/26204488 |
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