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Condensin Relocalization from Centromeres to Chromosome Arms Promotes Top2 Recruitment during Anaphase
Condensin is a conserved chromosomal complex necessary to promote mitotic chromosome condensation and sister chromatid resolution during anaphase. Here, we report that yeast condensin binds to replicated centromere regions. We show that centromeric condensin relocalizes to chromosome arms as cells u...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cell Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4695335/ https://www.ncbi.nlm.nih.gov/pubmed/26686624 http://dx.doi.org/10.1016/j.celrep.2015.11.041 |
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author | Leonard, Joanne Sen, Nicholas Torres, Raul Sutani, Takashi Jarmuz, Adam Shirahige, Katsuhiko Aragón, Luis |
author_facet | Leonard, Joanne Sen, Nicholas Torres, Raul Sutani, Takashi Jarmuz, Adam Shirahige, Katsuhiko Aragón, Luis |
author_sort | Leonard, Joanne |
collection | PubMed |
description | Condensin is a conserved chromosomal complex necessary to promote mitotic chromosome condensation and sister chromatid resolution during anaphase. Here, we report that yeast condensin binds to replicated centromere regions. We show that centromeric condensin relocalizes to chromosome arms as cells undergo anaphase segregation. We find that condensin relocalization is initiated immediately after the bipolar attachment of sister kinetochores to spindles and requires Polo kinase activity. Moreover, condensin localization during anaphase involves a higher binding rate on DNA and temporally overlaps with condensin’s DNA overwinding activity. Finally, we demonstrate that topoisomerase 2 (Top2) is also recruited to chromosome arms during anaphase in a condensin-dependent manner. Our results uncover a functional relation between condensin and Top2 during anaphase to mediate chromosome segregation. |
format | Online Article Text |
id | pubmed-4695335 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Cell Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-46953352016-01-31 Condensin Relocalization from Centromeres to Chromosome Arms Promotes Top2 Recruitment during Anaphase Leonard, Joanne Sen, Nicholas Torres, Raul Sutani, Takashi Jarmuz, Adam Shirahige, Katsuhiko Aragón, Luis Cell Rep Report Condensin is a conserved chromosomal complex necessary to promote mitotic chromosome condensation and sister chromatid resolution during anaphase. Here, we report that yeast condensin binds to replicated centromere regions. We show that centromeric condensin relocalizes to chromosome arms as cells undergo anaphase segregation. We find that condensin relocalization is initiated immediately after the bipolar attachment of sister kinetochores to spindles and requires Polo kinase activity. Moreover, condensin localization during anaphase involves a higher binding rate on DNA and temporally overlaps with condensin’s DNA overwinding activity. Finally, we demonstrate that topoisomerase 2 (Top2) is also recruited to chromosome arms during anaphase in a condensin-dependent manner. Our results uncover a functional relation between condensin and Top2 during anaphase to mediate chromosome segregation. Cell Press 2015-12-10 /pmc/articles/PMC4695335/ /pubmed/26686624 http://dx.doi.org/10.1016/j.celrep.2015.11.041 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Report Leonard, Joanne Sen, Nicholas Torres, Raul Sutani, Takashi Jarmuz, Adam Shirahige, Katsuhiko Aragón, Luis Condensin Relocalization from Centromeres to Chromosome Arms Promotes Top2 Recruitment during Anaphase |
title | Condensin Relocalization from Centromeres to Chromosome Arms Promotes Top2 Recruitment during Anaphase |
title_full | Condensin Relocalization from Centromeres to Chromosome Arms Promotes Top2 Recruitment during Anaphase |
title_fullStr | Condensin Relocalization from Centromeres to Chromosome Arms Promotes Top2 Recruitment during Anaphase |
title_full_unstemmed | Condensin Relocalization from Centromeres to Chromosome Arms Promotes Top2 Recruitment during Anaphase |
title_short | Condensin Relocalization from Centromeres to Chromosome Arms Promotes Top2 Recruitment during Anaphase |
title_sort | condensin relocalization from centromeres to chromosome arms promotes top2 recruitment during anaphase |
topic | Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4695335/ https://www.ncbi.nlm.nih.gov/pubmed/26686624 http://dx.doi.org/10.1016/j.celrep.2015.11.041 |
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