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Antifungal Susceptibility Testing with Etest for Candida Species Isolated from Patients with Oral Candidiasis
BACKGROUND: The necessity of performing antifungal susceptibility tests is recently increasing because of frequent cases of oral candidiasis caused by antifungal-resistant Candida species. The Etest (BioMerieux, Marcy l'Etoile, France) is a rapid and easy-to-perform in vitro antifungal suscepti...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Dermatological Association; The Korean Society for Investigative Dermatology
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4695424/ https://www.ncbi.nlm.nih.gov/pubmed/26719641 http://dx.doi.org/10.5021/ad.2015.27.6.715 |
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author | Song, You Bum Suh, Moo Kyu Ha, Gyoung Yim Kim, Heesoo |
author_facet | Song, You Bum Suh, Moo Kyu Ha, Gyoung Yim Kim, Heesoo |
author_sort | Song, You Bum |
collection | PubMed |
description | BACKGROUND: The necessity of performing antifungal susceptibility tests is recently increasing because of frequent cases of oral candidiasis caused by antifungal-resistant Candida species. The Etest (BioMerieux, Marcy l'Etoile, France) is a rapid and easy-to-perform in vitro antifungal susceptibility test. OBJECTIVE: The purpose of this study was to determine the minimal inhibitory concentrations (MICs) of antifungal agents by using the Etest for Candida species isolated from patients with oral candidiasis. METHODS: Forty-seven clinical isolates of Candida species (39 isolates of Candida albicans, 5 isolates of C. glabrata, and 3 isolates of C. tropicalis) were tested along with a reference strain (C. albicans ATCC 90028). The MIC end points of the Etest for fluconazole, itraconazole, voriconazole, and amphotericin B susceptibility were read after the 24-hour incubation of each isolate on RPMI 1640 agar. RESULTS: All Candida isolates were found susceptible to voriconazole and amphotericin B. However, all five isolates of C. glabrata were resistant to itraconazole, among which two isolates were also resistant to fluconazole. CONCLUSION: This study revealed that the Etest represented a simple and efficacious method for antifungal susceptibility testing of Candida species isolated from oral candidiasis patients. Therefore, voriconazole and amphotericin B should be recommended as effective alternatives for the treatment of oral candidiasis. |
format | Online Article Text |
id | pubmed-4695424 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Korean Dermatological Association; The Korean Society for Investigative Dermatology |
record_format | MEDLINE/PubMed |
spelling | pubmed-46954242015-12-30 Antifungal Susceptibility Testing with Etest for Candida Species Isolated from Patients with Oral Candidiasis Song, You Bum Suh, Moo Kyu Ha, Gyoung Yim Kim, Heesoo Ann Dermatol Original Article BACKGROUND: The necessity of performing antifungal susceptibility tests is recently increasing because of frequent cases of oral candidiasis caused by antifungal-resistant Candida species. The Etest (BioMerieux, Marcy l'Etoile, France) is a rapid and easy-to-perform in vitro antifungal susceptibility test. OBJECTIVE: The purpose of this study was to determine the minimal inhibitory concentrations (MICs) of antifungal agents by using the Etest for Candida species isolated from patients with oral candidiasis. METHODS: Forty-seven clinical isolates of Candida species (39 isolates of Candida albicans, 5 isolates of C. glabrata, and 3 isolates of C. tropicalis) were tested along with a reference strain (C. albicans ATCC 90028). The MIC end points of the Etest for fluconazole, itraconazole, voriconazole, and amphotericin B susceptibility were read after the 24-hour incubation of each isolate on RPMI 1640 agar. RESULTS: All Candida isolates were found susceptible to voriconazole and amphotericin B. However, all five isolates of C. glabrata were resistant to itraconazole, among which two isolates were also resistant to fluconazole. CONCLUSION: This study revealed that the Etest represented a simple and efficacious method for antifungal susceptibility testing of Candida species isolated from oral candidiasis patients. Therefore, voriconazole and amphotericin B should be recommended as effective alternatives for the treatment of oral candidiasis. Korean Dermatological Association; The Korean Society for Investigative Dermatology 2015-12 2015-12-07 /pmc/articles/PMC4695424/ /pubmed/26719641 http://dx.doi.org/10.5021/ad.2015.27.6.715 Text en Copyright © 2015 The Korean Dermatological Association and The Korean Society for Investigative Dermatology http://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Song, You Bum Suh, Moo Kyu Ha, Gyoung Yim Kim, Heesoo Antifungal Susceptibility Testing with Etest for Candida Species Isolated from Patients with Oral Candidiasis |
title | Antifungal Susceptibility Testing with Etest for Candida Species Isolated from Patients with Oral Candidiasis |
title_full | Antifungal Susceptibility Testing with Etest for Candida Species Isolated from Patients with Oral Candidiasis |
title_fullStr | Antifungal Susceptibility Testing with Etest for Candida Species Isolated from Patients with Oral Candidiasis |
title_full_unstemmed | Antifungal Susceptibility Testing with Etest for Candida Species Isolated from Patients with Oral Candidiasis |
title_short | Antifungal Susceptibility Testing with Etest for Candida Species Isolated from Patients with Oral Candidiasis |
title_sort | antifungal susceptibility testing with etest for candida species isolated from patients with oral candidiasis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4695424/ https://www.ncbi.nlm.nih.gov/pubmed/26719641 http://dx.doi.org/10.5021/ad.2015.27.6.715 |
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