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Phylogenetic investigation of enteric bovine coronavirus in Ireland reveals partitioning between European and global strains
BACKGROUND: Bovine coronavirus is a primary cause of neonatal calf diarrhea worldwide, and is also associated with acute diarrhea in adult cattle during the winter season. There are no reports on molecular characterization of bovine coronavirus in Ireland, and little data exists apart from serologic...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4696222/ https://www.ncbi.nlm.nih.gov/pubmed/26719792 http://dx.doi.org/10.1186/s13620-015-0060-3 |
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author | Gunn, L. Collins, P. J. O’Connell, M. J. O’Shea, H. |
author_facet | Gunn, L. Collins, P. J. O’Connell, M. J. O’Shea, H. |
author_sort | Gunn, L. |
collection | PubMed |
description | BACKGROUND: Bovine coronavirus is a primary cause of neonatal calf diarrhea worldwide, and is also associated with acute diarrhea in adult cattle during the winter season. There are no reports on molecular characterization of bovine coronavirus in Ireland, and little data exists apart from serological studies. FINDINGS: In this study, 11 neonatal (mean age 9 days) calf BCoV strains from the south of Ireland were collected over a one year period and characterized using molecular methods. The spike gene which encodes a protein involved in viral entry, infectivity and immune response shows the most variability amongst the isolates and was subsequently selected for in depth analysis. Phylogenetic analysis of the spike gene revealed that the Irish strains clustered with novel BCoV strains from Europe in a unique clade, possibly indicating lineage partitioning. Direct analysis of alignments identified amino acid changes in the spike protein unique to the Irish clade. CONCLUSION: Thus, monitoring of bovine coronavirus in Ireland is important as the current isolates in circulation in the south of Ireland may be diverging from the available vaccine strain, which may have implications regarding future BCoV vaccine efficacy. |
format | Online Article Text |
id | pubmed-4696222 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-46962222015-12-31 Phylogenetic investigation of enteric bovine coronavirus in Ireland reveals partitioning between European and global strains Gunn, L. Collins, P. J. O’Connell, M. J. O’Shea, H. Ir Vet J Short Report BACKGROUND: Bovine coronavirus is a primary cause of neonatal calf diarrhea worldwide, and is also associated with acute diarrhea in adult cattle during the winter season. There are no reports on molecular characterization of bovine coronavirus in Ireland, and little data exists apart from serological studies. FINDINGS: In this study, 11 neonatal (mean age 9 days) calf BCoV strains from the south of Ireland were collected over a one year period and characterized using molecular methods. The spike gene which encodes a protein involved in viral entry, infectivity and immune response shows the most variability amongst the isolates and was subsequently selected for in depth analysis. Phylogenetic analysis of the spike gene revealed that the Irish strains clustered with novel BCoV strains from Europe in a unique clade, possibly indicating lineage partitioning. Direct analysis of alignments identified amino acid changes in the spike protein unique to the Irish clade. CONCLUSION: Thus, monitoring of bovine coronavirus in Ireland is important as the current isolates in circulation in the south of Ireland may be diverging from the available vaccine strain, which may have implications regarding future BCoV vaccine efficacy. BioMed Central 2015-12-30 /pmc/articles/PMC4696222/ /pubmed/26719792 http://dx.doi.org/10.1186/s13620-015-0060-3 Text en © Gunn et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Short Report Gunn, L. Collins, P. J. O’Connell, M. J. O’Shea, H. Phylogenetic investigation of enteric bovine coronavirus in Ireland reveals partitioning between European and global strains |
title | Phylogenetic investigation of enteric bovine coronavirus in Ireland reveals partitioning between European and global strains |
title_full | Phylogenetic investigation of enteric bovine coronavirus in Ireland reveals partitioning between European and global strains |
title_fullStr | Phylogenetic investigation of enteric bovine coronavirus in Ireland reveals partitioning between European and global strains |
title_full_unstemmed | Phylogenetic investigation of enteric bovine coronavirus in Ireland reveals partitioning between European and global strains |
title_short | Phylogenetic investigation of enteric bovine coronavirus in Ireland reveals partitioning between European and global strains |
title_sort | phylogenetic investigation of enteric bovine coronavirus in ireland reveals partitioning between european and global strains |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4696222/ https://www.ncbi.nlm.nih.gov/pubmed/26719792 http://dx.doi.org/10.1186/s13620-015-0060-3 |
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