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Lack of association among TNF-α gene expression, -308 polymorphism (G > A) and virulence markers of Helicobacter pylori
BACKGROUND: Tumor necrosis factor plays a critical role in the pathogenesis of gastric diseases such as gastric cancer, and an abnormal inflammatory response has frequently been observed in dyspeptic patients. Helicobacter pylori infection can induce a gastric mucosal inflammatory response that may...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4696262/ https://www.ncbi.nlm.nih.gov/pubmed/26719751 http://dx.doi.org/10.1186/s40409-015-0054-3 |
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author | Zabaglia, Luanna Munhoz Ferraz, Mariane Avante Pereira, Weendelly Nayara Orcini, Wilson Aparecido de Labio, Roger Willian Neto, Agostinho Caleman Wisnieski, Fernanda de Oliveira, Juliana Garcia de Arruda Cardoso Smith, Marilia Payão, Spencer Luiz Marques Rasmussen, Lucas Trevizani |
author_facet | Zabaglia, Luanna Munhoz Ferraz, Mariane Avante Pereira, Weendelly Nayara Orcini, Wilson Aparecido de Labio, Roger Willian Neto, Agostinho Caleman Wisnieski, Fernanda de Oliveira, Juliana Garcia de Arruda Cardoso Smith, Marilia Payão, Spencer Luiz Marques Rasmussen, Lucas Trevizani |
author_sort | Zabaglia, Luanna Munhoz |
collection | PubMed |
description | BACKGROUND: Tumor necrosis factor plays a critical role in the pathogenesis of gastric diseases such as gastric cancer, and an abnormal inflammatory response has frequently been observed in dyspeptic patients. Helicobacter pylori infection can induce a gastric mucosal inflammatory response that may be influenced by -308 (G > A) polymorphisms and gene expression of the TNF-α gene. METHODS: One hundred and thirty-four gastric biopsy samples were collected from patients of both genders (61♂ and 73♀, mean age 40.3 ± 24.2 years) with gastric symptoms. The -308 (G > A) polymorphism of TNF-α was characterized using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). The expression level was measured using real-time PCR, and relative quantification (RQ) was calculated using the comparative CT method (2(-ΔΔCT)). RESULTS: The analysis revealed an increase in TNF-α gene expression in patients with gastritis; on the other hand, no statistical differences were observed in patients with gastric cancer. In addition, no association was found among -308 polymorphism genotypes, virulence markers, or TNF-α gene expression. CONCLUSIONS: Helicobacter pylori induces a large increase in TNF-α expression in patients with gastritis, regardless of tissue inflammation, but after the tissue becomes neoplastic, the presence of bacteria did not influence expression. These results suggest that the TNF-α pathway may play an important role in the progression from gastritis to gastric cancer |
format | Online Article Text |
id | pubmed-4696262 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-46962622015-12-31 Lack of association among TNF-α gene expression, -308 polymorphism (G > A) and virulence markers of Helicobacter pylori Zabaglia, Luanna Munhoz Ferraz, Mariane Avante Pereira, Weendelly Nayara Orcini, Wilson Aparecido de Labio, Roger Willian Neto, Agostinho Caleman Wisnieski, Fernanda de Oliveira, Juliana Garcia de Arruda Cardoso Smith, Marilia Payão, Spencer Luiz Marques Rasmussen, Lucas Trevizani J Venom Anim Toxins Incl Trop Dis Research BACKGROUND: Tumor necrosis factor plays a critical role in the pathogenesis of gastric diseases such as gastric cancer, and an abnormal inflammatory response has frequently been observed in dyspeptic patients. Helicobacter pylori infection can induce a gastric mucosal inflammatory response that may be influenced by -308 (G > A) polymorphisms and gene expression of the TNF-α gene. METHODS: One hundred and thirty-four gastric biopsy samples were collected from patients of both genders (61♂ and 73♀, mean age 40.3 ± 24.2 years) with gastric symptoms. The -308 (G > A) polymorphism of TNF-α was characterized using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). The expression level was measured using real-time PCR, and relative quantification (RQ) was calculated using the comparative CT method (2(-ΔΔCT)). RESULTS: The analysis revealed an increase in TNF-α gene expression in patients with gastritis; on the other hand, no statistical differences were observed in patients with gastric cancer. In addition, no association was found among -308 polymorphism genotypes, virulence markers, or TNF-α gene expression. CONCLUSIONS: Helicobacter pylori induces a large increase in TNF-α expression in patients with gastritis, regardless of tissue inflammation, but after the tissue becomes neoplastic, the presence of bacteria did not influence expression. These results suggest that the TNF-α pathway may play an important role in the progression from gastritis to gastric cancer BioMed Central 2015-12-30 /pmc/articles/PMC4696262/ /pubmed/26719751 http://dx.doi.org/10.1186/s40409-015-0054-3 Text en © Zabaglia et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Zabaglia, Luanna Munhoz Ferraz, Mariane Avante Pereira, Weendelly Nayara Orcini, Wilson Aparecido de Labio, Roger Willian Neto, Agostinho Caleman Wisnieski, Fernanda de Oliveira, Juliana Garcia de Arruda Cardoso Smith, Marilia Payão, Spencer Luiz Marques Rasmussen, Lucas Trevizani Lack of association among TNF-α gene expression, -308 polymorphism (G > A) and virulence markers of Helicobacter pylori |
title | Lack of association among TNF-α gene expression, -308 polymorphism (G > A) and virulence markers of Helicobacter pylori |
title_full | Lack of association among TNF-α gene expression, -308 polymorphism (G > A) and virulence markers of Helicobacter pylori |
title_fullStr | Lack of association among TNF-α gene expression, -308 polymorphism (G > A) and virulence markers of Helicobacter pylori |
title_full_unstemmed | Lack of association among TNF-α gene expression, -308 polymorphism (G > A) and virulence markers of Helicobacter pylori |
title_short | Lack of association among TNF-α gene expression, -308 polymorphism (G > A) and virulence markers of Helicobacter pylori |
title_sort | lack of association among tnf-α gene expression, -308 polymorphism (g > a) and virulence markers of helicobacter pylori |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4696262/ https://www.ncbi.nlm.nih.gov/pubmed/26719751 http://dx.doi.org/10.1186/s40409-015-0054-3 |
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