Extensive Hair Shaft Growth after Mouse Whisker Follicle Isolation, Cryopreservation and Transplantation in Nude Mice

We previously demonstrated that whole hair follicles could be cryopreserved to maintain their stem-cells differentation potential. In the present study, we demonstrated that cryopreserved mouse whisker hair follicles maintain their hair growth potential. DMSO better cryopreserved mouse whisker folli...

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Detalles Bibliográficos
Autores principales: Cao, Wenluo, Li, Lingna, Tran, Benjamin, Kajiura, Satoshi, Amoh, Yasuyuki, Liu, Fang, Hoffman, Robert M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4696652/
https://www.ncbi.nlm.nih.gov/pubmed/26716690
http://dx.doi.org/10.1371/journal.pone.0145997
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author Cao, Wenluo
Li, Lingna
Tran, Benjamin
Kajiura, Satoshi
Amoh, Yasuyuki
Liu, Fang
Hoffman, Robert M.
author_facet Cao, Wenluo
Li, Lingna
Tran, Benjamin
Kajiura, Satoshi
Amoh, Yasuyuki
Liu, Fang
Hoffman, Robert M.
author_sort Cao, Wenluo
collection PubMed
description We previously demonstrated that whole hair follicles could be cryopreserved to maintain their stem-cells differentation potential. In the present study, we demonstrated that cryopreserved mouse whisker hair follicles maintain their hair growth potential. DMSO better cryopreserved mouse whisker follicles compared to glycerol. Cryopreserved hair follicles also maintained the hair follicle-associated-pluripotent (HAP) stem cells, evidenced by P75(NTR) expression. Subcutaneous transplantation of DMSO-cryopreserved hair follicles in nude mice resulted in extensive hair fiber growth over 8 weeks, indicating the functional recovery of hair shaft growth of cryopreserved hair follicles.
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spelling pubmed-46966522016-01-13 Extensive Hair Shaft Growth after Mouse Whisker Follicle Isolation, Cryopreservation and Transplantation in Nude Mice Cao, Wenluo Li, Lingna Tran, Benjamin Kajiura, Satoshi Amoh, Yasuyuki Liu, Fang Hoffman, Robert M. PLoS One Research Article We previously demonstrated that whole hair follicles could be cryopreserved to maintain their stem-cells differentation potential. In the present study, we demonstrated that cryopreserved mouse whisker hair follicles maintain their hair growth potential. DMSO better cryopreserved mouse whisker follicles compared to glycerol. Cryopreserved hair follicles also maintained the hair follicle-associated-pluripotent (HAP) stem cells, evidenced by P75(NTR) expression. Subcutaneous transplantation of DMSO-cryopreserved hair follicles in nude mice resulted in extensive hair fiber growth over 8 weeks, indicating the functional recovery of hair shaft growth of cryopreserved hair follicles. Public Library of Science 2015-12-30 /pmc/articles/PMC4696652/ /pubmed/26716690 http://dx.doi.org/10.1371/journal.pone.0145997 Text en © 2015 Cao et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Cao, Wenluo
Li, Lingna
Tran, Benjamin
Kajiura, Satoshi
Amoh, Yasuyuki
Liu, Fang
Hoffman, Robert M.
Extensive Hair Shaft Growth after Mouse Whisker Follicle Isolation, Cryopreservation and Transplantation in Nude Mice
title Extensive Hair Shaft Growth after Mouse Whisker Follicle Isolation, Cryopreservation and Transplantation in Nude Mice
title_full Extensive Hair Shaft Growth after Mouse Whisker Follicle Isolation, Cryopreservation and Transplantation in Nude Mice
title_fullStr Extensive Hair Shaft Growth after Mouse Whisker Follicle Isolation, Cryopreservation and Transplantation in Nude Mice
title_full_unstemmed Extensive Hair Shaft Growth after Mouse Whisker Follicle Isolation, Cryopreservation and Transplantation in Nude Mice
title_short Extensive Hair Shaft Growth after Mouse Whisker Follicle Isolation, Cryopreservation and Transplantation in Nude Mice
title_sort extensive hair shaft growth after mouse whisker follicle isolation, cryopreservation and transplantation in nude mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4696652/
https://www.ncbi.nlm.nih.gov/pubmed/26716690
http://dx.doi.org/10.1371/journal.pone.0145997
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