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Genetic Evidence for the Role of the Vacuole in Supplying Secretory Organelles with Ca(2+) in Hansenula polymorpha

Processes taking place in the secretory organelles require Ca(2+) and Mn(2+), which in yeast are supplied by the Pmr1 ion pump. Here we observed that in the yeast Hansenula polymorpha Ca(2+) deficiency in the secretory pathway caused by Pmr1 inactivation is exacerbated by (i) the ret1-27 mutation af...

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Autores principales: Fokina, Anastasia V., Chechenova, Maria B., Karginov, Azamat V., Ter-Avanesyan, Michael D., Agaphonov, Michael O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4696657/
https://www.ncbi.nlm.nih.gov/pubmed/26717478
http://dx.doi.org/10.1371/journal.pone.0145915
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author Fokina, Anastasia V.
Chechenova, Maria B.
Karginov, Azamat V.
Ter-Avanesyan, Michael D.
Agaphonov, Michael O.
author_facet Fokina, Anastasia V.
Chechenova, Maria B.
Karginov, Azamat V.
Ter-Avanesyan, Michael D.
Agaphonov, Michael O.
author_sort Fokina, Anastasia V.
collection PubMed
description Processes taking place in the secretory organelles require Ca(2+) and Mn(2+), which in yeast are supplied by the Pmr1 ion pump. Here we observed that in the yeast Hansenula polymorpha Ca(2+) deficiency in the secretory pathway caused by Pmr1 inactivation is exacerbated by (i) the ret1-27 mutation affecting COPI-mediated vesicular transport, (ii) inactivation of the vacuolar Ca(2+) ATPase Pmc1 and (iii) inactivation of Vps35, which is a component of the retromer complex responsible for protein transport between the vacuole and secretory organelles. The ret1-27 mutation also exerted phenotypes indicating alterations in transport between the vacuole and secretory organelles. These data indicate that ret1-27, pmc1 and vps35 affect a previously unknown Pmr1-independent route of the Ca(2+) delivery to the secretory pathway. We also observed that the vacuolar protein carboxypeptidase Y receives additional modifications of its glycoside chains if it escapes the Vps10-dependent sorting to the vacuole.
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spelling pubmed-46966572016-01-13 Genetic Evidence for the Role of the Vacuole in Supplying Secretory Organelles with Ca(2+) in Hansenula polymorpha Fokina, Anastasia V. Chechenova, Maria B. Karginov, Azamat V. Ter-Avanesyan, Michael D. Agaphonov, Michael O. PLoS One Research Article Processes taking place in the secretory organelles require Ca(2+) and Mn(2+), which in yeast are supplied by the Pmr1 ion pump. Here we observed that in the yeast Hansenula polymorpha Ca(2+) deficiency in the secretory pathway caused by Pmr1 inactivation is exacerbated by (i) the ret1-27 mutation affecting COPI-mediated vesicular transport, (ii) inactivation of the vacuolar Ca(2+) ATPase Pmc1 and (iii) inactivation of Vps35, which is a component of the retromer complex responsible for protein transport between the vacuole and secretory organelles. The ret1-27 mutation also exerted phenotypes indicating alterations in transport between the vacuole and secretory organelles. These data indicate that ret1-27, pmc1 and vps35 affect a previously unknown Pmr1-independent route of the Ca(2+) delivery to the secretory pathway. We also observed that the vacuolar protein carboxypeptidase Y receives additional modifications of its glycoside chains if it escapes the Vps10-dependent sorting to the vacuole. Public Library of Science 2015-12-30 /pmc/articles/PMC4696657/ /pubmed/26717478 http://dx.doi.org/10.1371/journal.pone.0145915 Text en © 2015 Fokina et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Fokina, Anastasia V.
Chechenova, Maria B.
Karginov, Azamat V.
Ter-Avanesyan, Michael D.
Agaphonov, Michael O.
Genetic Evidence for the Role of the Vacuole in Supplying Secretory Organelles with Ca(2+) in Hansenula polymorpha
title Genetic Evidence for the Role of the Vacuole in Supplying Secretory Organelles with Ca(2+) in Hansenula polymorpha
title_full Genetic Evidence for the Role of the Vacuole in Supplying Secretory Organelles with Ca(2+) in Hansenula polymorpha
title_fullStr Genetic Evidence for the Role of the Vacuole in Supplying Secretory Organelles with Ca(2+) in Hansenula polymorpha
title_full_unstemmed Genetic Evidence for the Role of the Vacuole in Supplying Secretory Organelles with Ca(2+) in Hansenula polymorpha
title_short Genetic Evidence for the Role of the Vacuole in Supplying Secretory Organelles with Ca(2+) in Hansenula polymorpha
title_sort genetic evidence for the role of the vacuole in supplying secretory organelles with ca(2+) in hansenula polymorpha
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4696657/
https://www.ncbi.nlm.nih.gov/pubmed/26717478
http://dx.doi.org/10.1371/journal.pone.0145915
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