Cargando…
Extensive Modulation of the Fecal Metagenome in Children With Crohn's Disease During Exclusive Enteral Nutrition
OBJECTIVES: Exploring associations between the gut microbiota and colonic inflammation and assessing sequential changes during exclusive enteral nutrition (EEN) may offer clues into the microbial origins of Crohn's disease (CD). METHODS: Fecal samples (n=117) were collected from 23 CD and 21 he...
Autores principales: | , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4697132/ https://www.ncbi.nlm.nih.gov/pubmed/26526081 http://dx.doi.org/10.1038/ajg.2015.357 |
_version_ | 1782407891126321152 |
---|---|
author | Quince, Christopher Ijaz, Umer Zeeshan Loman, Nick Eren, A Murat Saulnier, Delphine Russell, Julie Haig, Sarah J Calus, Szymon T Quick, Joshua Barclay, Andrew Bertz, Martin Blaut, Michael Hansen, Richard McGrogan, Paraic Russell, Richard K Edwards, Christine A Gerasimidis, Konstantinos |
author_facet | Quince, Christopher Ijaz, Umer Zeeshan Loman, Nick Eren, A Murat Saulnier, Delphine Russell, Julie Haig, Sarah J Calus, Szymon T Quick, Joshua Barclay, Andrew Bertz, Martin Blaut, Michael Hansen, Richard McGrogan, Paraic Russell, Richard K Edwards, Christine A Gerasimidis, Konstantinos |
author_sort | Quince, Christopher |
collection | PubMed |
description | OBJECTIVES: Exploring associations between the gut microbiota and colonic inflammation and assessing sequential changes during exclusive enteral nutrition (EEN) may offer clues into the microbial origins of Crohn's disease (CD). METHODS: Fecal samples (n=117) were collected from 23 CD and 21 healthy children. From CD children fecal samples were collected before, during EEN, and when patients returned to their habitual diets. Microbiota composition and functional capacity were characterized using sequencing of the 16S rRNA gene and shotgun metagenomics. RESULTS: Microbial diversity was lower in CD than controls before EEN (P=0.006); differences were observed in 36 genera, 141 operational taxonomic units (OTUs), and 44 oligotypes. During EEN, the microbial diversity of CD children further decreased, and the community structure became even more dissimilar than that of controls. Every 10 days on EEN, 0.6 genus diversity equivalents were lost; 34 genera decreased and one increased during EEN. Fecal calprotectin correlated with 35 OTUs, 14 of which accounted for 78% of its variation. OTUs that correlated positively or negatively with calprotectin decreased during EEN. The microbiota of CD patients had a broader functional capacity than healthy controls, but diversity decreased with EEN. Genes involved in membrane transport, sulfur reduction, and nutrient biosynthesis differed between patients and controls. The abundance of genes involved in biotin (P=0.005) and thiamine biosynthesis decreased (P=0.017), whereas those involved in spermidine/putrescine biosynthesis (P=0.031), or the shikimate pathway (P=0.058), increased during EEN. CONCLUSIONS: Disease improvement following treatment with EEN is associated with extensive modulation of the gut microbiome. |
format | Online Article Text |
id | pubmed-4697132 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-46971322016-01-05 Extensive Modulation of the Fecal Metagenome in Children With Crohn's Disease During Exclusive Enteral Nutrition Quince, Christopher Ijaz, Umer Zeeshan Loman, Nick Eren, A Murat Saulnier, Delphine Russell, Julie Haig, Sarah J Calus, Szymon T Quick, Joshua Barclay, Andrew Bertz, Martin Blaut, Michael Hansen, Richard McGrogan, Paraic Russell, Richard K Edwards, Christine A Gerasimidis, Konstantinos Am J Gastroenterol Pediatrics OBJECTIVES: Exploring associations between the gut microbiota and colonic inflammation and assessing sequential changes during exclusive enteral nutrition (EEN) may offer clues into the microbial origins of Crohn's disease (CD). METHODS: Fecal samples (n=117) were collected from 23 CD and 21 healthy children. From CD children fecal samples were collected before, during EEN, and when patients returned to their habitual diets. Microbiota composition and functional capacity were characterized using sequencing of the 16S rRNA gene and shotgun metagenomics. RESULTS: Microbial diversity was lower in CD than controls before EEN (P=0.006); differences were observed in 36 genera, 141 operational taxonomic units (OTUs), and 44 oligotypes. During EEN, the microbial diversity of CD children further decreased, and the community structure became even more dissimilar than that of controls. Every 10 days on EEN, 0.6 genus diversity equivalents were lost; 34 genera decreased and one increased during EEN. Fecal calprotectin correlated with 35 OTUs, 14 of which accounted for 78% of its variation. OTUs that correlated positively or negatively with calprotectin decreased during EEN. The microbiota of CD patients had a broader functional capacity than healthy controls, but diversity decreased with EEN. Genes involved in membrane transport, sulfur reduction, and nutrient biosynthesis differed between patients and controls. The abundance of genes involved in biotin (P=0.005) and thiamine biosynthesis decreased (P=0.017), whereas those involved in spermidine/putrescine biosynthesis (P=0.031), or the shikimate pathway (P=0.058), increased during EEN. CONCLUSIONS: Disease improvement following treatment with EEN is associated with extensive modulation of the gut microbiome. Nature Publishing Group 2015-12 2015-11-03 /pmc/articles/PMC4697132/ /pubmed/26526081 http://dx.doi.org/10.1038/ajg.2015.357 Text en Copyright © 2015 American College of Gastroenterology http://creativecommons.org/licenses/by-nc-sa/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/ |
spellingShingle | Pediatrics Quince, Christopher Ijaz, Umer Zeeshan Loman, Nick Eren, A Murat Saulnier, Delphine Russell, Julie Haig, Sarah J Calus, Szymon T Quick, Joshua Barclay, Andrew Bertz, Martin Blaut, Michael Hansen, Richard McGrogan, Paraic Russell, Richard K Edwards, Christine A Gerasimidis, Konstantinos Extensive Modulation of the Fecal Metagenome in Children With Crohn's Disease During Exclusive Enteral Nutrition |
title | Extensive Modulation of the Fecal Metagenome in Children With Crohn's Disease During Exclusive Enteral Nutrition |
title_full | Extensive Modulation of the Fecal Metagenome in Children With Crohn's Disease During Exclusive Enteral Nutrition |
title_fullStr | Extensive Modulation of the Fecal Metagenome in Children With Crohn's Disease During Exclusive Enteral Nutrition |
title_full_unstemmed | Extensive Modulation of the Fecal Metagenome in Children With Crohn's Disease During Exclusive Enteral Nutrition |
title_short | Extensive Modulation of the Fecal Metagenome in Children With Crohn's Disease During Exclusive Enteral Nutrition |
title_sort | extensive modulation of the fecal metagenome in children with crohn's disease during exclusive enteral nutrition |
topic | Pediatrics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4697132/ https://www.ncbi.nlm.nih.gov/pubmed/26526081 http://dx.doi.org/10.1038/ajg.2015.357 |
work_keys_str_mv | AT quincechristopher extensivemodulationofthefecalmetagenomeinchildrenwithcrohnsdiseaseduringexclusiveenteralnutrition AT ijazumerzeeshan extensivemodulationofthefecalmetagenomeinchildrenwithcrohnsdiseaseduringexclusiveenteralnutrition AT lomannick extensivemodulationofthefecalmetagenomeinchildrenwithcrohnsdiseaseduringexclusiveenteralnutrition AT erenamurat extensivemodulationofthefecalmetagenomeinchildrenwithcrohnsdiseaseduringexclusiveenteralnutrition AT saulnierdelphine extensivemodulationofthefecalmetagenomeinchildrenwithcrohnsdiseaseduringexclusiveenteralnutrition AT russelljulie extensivemodulationofthefecalmetagenomeinchildrenwithcrohnsdiseaseduringexclusiveenteralnutrition AT haigsarahj extensivemodulationofthefecalmetagenomeinchildrenwithcrohnsdiseaseduringexclusiveenteralnutrition AT calusszymont extensivemodulationofthefecalmetagenomeinchildrenwithcrohnsdiseaseduringexclusiveenteralnutrition AT quickjoshua extensivemodulationofthefecalmetagenomeinchildrenwithcrohnsdiseaseduringexclusiveenteralnutrition AT barclayandrew extensivemodulationofthefecalmetagenomeinchildrenwithcrohnsdiseaseduringexclusiveenteralnutrition AT bertzmartin extensivemodulationofthefecalmetagenomeinchildrenwithcrohnsdiseaseduringexclusiveenteralnutrition AT blautmichael extensivemodulationofthefecalmetagenomeinchildrenwithcrohnsdiseaseduringexclusiveenteralnutrition AT hansenrichard extensivemodulationofthefecalmetagenomeinchildrenwithcrohnsdiseaseduringexclusiveenteralnutrition AT mcgroganparaic extensivemodulationofthefecalmetagenomeinchildrenwithcrohnsdiseaseduringexclusiveenteralnutrition AT russellrichardk extensivemodulationofthefecalmetagenomeinchildrenwithcrohnsdiseaseduringexclusiveenteralnutrition AT edwardschristinea extensivemodulationofthefecalmetagenomeinchildrenwithcrohnsdiseaseduringexclusiveenteralnutrition AT gerasimidiskonstantinos extensivemodulationofthefecalmetagenomeinchildrenwithcrohnsdiseaseduringexclusiveenteralnutrition |