A Ferredoxin Disulfide Reductase Delivers Electrons to the Methanosarcina barkeri Class III Ribonucleotide Reductase

[Image: see text] Two subtypes of class III anaerobic ribonucleotide reductases (RNRs) studied so far couple the reduction of ribonucleotides to the oxidation of formate, or the oxidation of NADPH via thioredoxin and thioredoxin reductase. Certain methanogenic archaea contain a phylogenetically dist...

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Autores principales: Wei, Yifeng, Li, Bin, Prakash, Divya, Ferry, James G., Elliott, Sean J., Stubbe, JoAnne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2015
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4697749/
https://www.ncbi.nlm.nih.gov/pubmed/26536144
http://dx.doi.org/10.1021/acs.biochem.5b01092
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author Wei, Yifeng
Li, Bin
Prakash, Divya
Ferry, James G.
Elliott, Sean J.
Stubbe, JoAnne
author_facet Wei, Yifeng
Li, Bin
Prakash, Divya
Ferry, James G.
Elliott, Sean J.
Stubbe, JoAnne
author_sort Wei, Yifeng
collection PubMed
description [Image: see text] Two subtypes of class III anaerobic ribonucleotide reductases (RNRs) studied so far couple the reduction of ribonucleotides to the oxidation of formate, or the oxidation of NADPH via thioredoxin and thioredoxin reductase. Certain methanogenic archaea contain a phylogenetically distinct third subtype of class III RNR, with distinct active-site residues. Here we report the cloning and recombinant expression of the Methanosarcina barkeri class III RNR and show that the electrons required for ribonucleotide reduction can be delivered by a [4Fe-4S] protein ferredoxin disulfide reductase, and a conserved thioredoxin-like protein NrdH present in the RNR operon. The diversity of class III RNRs reflects the diversity of electron carriers used in anaerobic metabolism.
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spelling pubmed-46977492016-11-04 A Ferredoxin Disulfide Reductase Delivers Electrons to the Methanosarcina barkeri Class III Ribonucleotide Reductase Wei, Yifeng Li, Bin Prakash, Divya Ferry, James G. Elliott, Sean J. Stubbe, JoAnne Biochemistry [Image: see text] Two subtypes of class III anaerobic ribonucleotide reductases (RNRs) studied so far couple the reduction of ribonucleotides to the oxidation of formate, or the oxidation of NADPH via thioredoxin and thioredoxin reductase. Certain methanogenic archaea contain a phylogenetically distinct third subtype of class III RNR, with distinct active-site residues. Here we report the cloning and recombinant expression of the Methanosarcina barkeri class III RNR and show that the electrons required for ribonucleotide reduction can be delivered by a [4Fe-4S] protein ferredoxin disulfide reductase, and a conserved thioredoxin-like protein NrdH present in the RNR operon. The diversity of class III RNRs reflects the diversity of electron carriers used in anaerobic metabolism. American Chemical Society 2015-11-04 2015-12-01 /pmc/articles/PMC4697749/ /pubmed/26536144 http://dx.doi.org/10.1021/acs.biochem.5b01092 Text en Copyright © 2015 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Wei, Yifeng
Li, Bin
Prakash, Divya
Ferry, James G.
Elliott, Sean J.
Stubbe, JoAnne
A Ferredoxin Disulfide Reductase Delivers Electrons to the Methanosarcina barkeri Class III Ribonucleotide Reductase
title A Ferredoxin Disulfide Reductase Delivers Electrons to the Methanosarcina barkeri Class III Ribonucleotide Reductase
title_full A Ferredoxin Disulfide Reductase Delivers Electrons to the Methanosarcina barkeri Class III Ribonucleotide Reductase
title_fullStr A Ferredoxin Disulfide Reductase Delivers Electrons to the Methanosarcina barkeri Class III Ribonucleotide Reductase
title_full_unstemmed A Ferredoxin Disulfide Reductase Delivers Electrons to the Methanosarcina barkeri Class III Ribonucleotide Reductase
title_short A Ferredoxin Disulfide Reductase Delivers Electrons to the Methanosarcina barkeri Class III Ribonucleotide Reductase
title_sort ferredoxin disulfide reductase delivers electrons to the methanosarcina barkeri class iii ribonucleotide reductase
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4697749/
https://www.ncbi.nlm.nih.gov/pubmed/26536144
http://dx.doi.org/10.1021/acs.biochem.5b01092
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