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Chromosomal distribution of microsatellite repeats in Amazon cichlids genome (Pisces, Cichlidae)

Abstract. Fish of the family Cichlidae are recognized as an excellent model for evolutionary studies because of their morphological and behavioral adaptations to a wide diversity of explored ecological niches. In addition, the family has a dynamic genome with variable structure, composition and kary...

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Autores principales: Schneider, Carlos Henrique, Gross, Maria Claudia, Terencio, Maria Leandra, de Tavares, Édika Sabrina Girão Mitozo, Martins, Cesar, Feldberg, Eliana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pensoft Publishers 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4698573/
https://www.ncbi.nlm.nih.gov/pubmed/26753076
http://dx.doi.org/10.3897/CompCytogen.v9i4.5582
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author Schneider, Carlos Henrique
Gross, Maria Claudia
Terencio, Maria Leandra
de Tavares, Édika Sabrina Girão Mitozo
Martins, Cesar
Feldberg, Eliana
author_facet Schneider, Carlos Henrique
Gross, Maria Claudia
Terencio, Maria Leandra
de Tavares, Édika Sabrina Girão Mitozo
Martins, Cesar
Feldberg, Eliana
author_sort Schneider, Carlos Henrique
collection PubMed
description Abstract. Fish of the family Cichlidae are recognized as an excellent model for evolutionary studies because of their morphological and behavioral adaptations to a wide diversity of explored ecological niches. In addition, the family has a dynamic genome with variable structure, composition and karyotype organization. Microsatellites represent the most dynamic genomic component and a better understanding of their organization may help clarify the role of repetitive DNA elements in the mechanisms of chromosomal evolution. Thus, in this study, microsatellite sequences were mapped in the chromosomes of Cichla monoculus Agassiz, 1831, Pterophyllum scalare Schultze, 1823, and Symphysodon discus Heckel, 1840. Four microsatellites demonstrated positive results in the genome of Cichla monoculus and Symphysodon discus, and five demonstrated positive results in the genome of Pterophyllum scalare. In most cases, the microsatellite was dispersed in the chromosome with conspicuous markings in the centromeric or telomeric regions, which suggests that sequences contribute to chromosome structure and may have played a role in the evolution of this fish family. The comparative genome mapping data presented here provide novel information on the structure and organization of the repetitive DNA region of the cichlid genome and contribute to a better understanding of this fish family’s genome.
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spelling pubmed-46985732016-01-08 Chromosomal distribution of microsatellite repeats in Amazon cichlids genome (Pisces, Cichlidae) Schneider, Carlos Henrique Gross, Maria Claudia Terencio, Maria Leandra de Tavares, Édika Sabrina Girão Mitozo Martins, Cesar Feldberg, Eliana Comp Cytogenet Research Articles Abstract. Fish of the family Cichlidae are recognized as an excellent model for evolutionary studies because of their morphological and behavioral adaptations to a wide diversity of explored ecological niches. In addition, the family has a dynamic genome with variable structure, composition and karyotype organization. Microsatellites represent the most dynamic genomic component and a better understanding of their organization may help clarify the role of repetitive DNA elements in the mechanisms of chromosomal evolution. Thus, in this study, microsatellite sequences were mapped in the chromosomes of Cichla monoculus Agassiz, 1831, Pterophyllum scalare Schultze, 1823, and Symphysodon discus Heckel, 1840. Four microsatellites demonstrated positive results in the genome of Cichla monoculus and Symphysodon discus, and five demonstrated positive results in the genome of Pterophyllum scalare. In most cases, the microsatellite was dispersed in the chromosome with conspicuous markings in the centromeric or telomeric regions, which suggests that sequences contribute to chromosome structure and may have played a role in the evolution of this fish family. The comparative genome mapping data presented here provide novel information on the structure and organization of the repetitive DNA region of the cichlid genome and contribute to a better understanding of this fish family’s genome. Pensoft Publishers 2015-09-14 /pmc/articles/PMC4698573/ /pubmed/26753076 http://dx.doi.org/10.3897/CompCytogen.v9i4.5582 Text en Carlos Henrique Schneider, Maria Claudia Gross, Maria Leandra Terencio, Édika Sabrina Girão Mitozo de Tavares, Cesar Martins, Eliana Feldberg http://creativecommons.org/licenses/by/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Articles
Schneider, Carlos Henrique
Gross, Maria Claudia
Terencio, Maria Leandra
de Tavares, Édika Sabrina Girão Mitozo
Martins, Cesar
Feldberg, Eliana
Chromosomal distribution of microsatellite repeats in Amazon cichlids genome (Pisces, Cichlidae)
title Chromosomal distribution of microsatellite repeats in Amazon cichlids genome (Pisces, Cichlidae)
title_full Chromosomal distribution of microsatellite repeats in Amazon cichlids genome (Pisces, Cichlidae)
title_fullStr Chromosomal distribution of microsatellite repeats in Amazon cichlids genome (Pisces, Cichlidae)
title_full_unstemmed Chromosomal distribution of microsatellite repeats in Amazon cichlids genome (Pisces, Cichlidae)
title_short Chromosomal distribution of microsatellite repeats in Amazon cichlids genome (Pisces, Cichlidae)
title_sort chromosomal distribution of microsatellite repeats in amazon cichlids genome (pisces, cichlidae)
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4698573/
https://www.ncbi.nlm.nih.gov/pubmed/26753076
http://dx.doi.org/10.3897/CompCytogen.v9i4.5582
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