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Influence of Temperature on Growth and Production of Pectenotoxin-2 by a Monoclonal Culture of Dinophysis caudata

The effects of temperature on growth and production of Lipophilic Toxins (LT) by a monoclonal culture of Dinophysis caudata was studied. The cell density of D. caudata increased significantly with increasing temperature, and was the highest under 27, 30, and 32.5 °C. Temperature affected the average...

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Detalles Bibliográficos
Autores principales: Basti, Leila, Uchida, Hajime, Matsushima, Ryoji, Watanabe, Ryuichi, Suzuki, Toshiyuki, Yamatogi, Toshifumi, Nagai, Satoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4699234/
https://www.ncbi.nlm.nih.gov/pubmed/26633427
http://dx.doi.org/10.3390/md13127061
Descripción
Sumario:The effects of temperature on growth and production of Lipophilic Toxins (LT) by a monoclonal culture of Dinophysis caudata was studied. The cell density of D. caudata increased significantly with increasing temperature, and was the highest under 27, 30, and 32.5 °C. Temperature affected the average specific growth rate (µ) during the exponential growth phase (EG), which increased from 15 °C to 30 °C, and then decreased at 32.5 °C. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) revealed that this strain of D. caudata produced only pectenotoxin-2 (PTX-2) whose concentration increased significantly with incubation period, except at 32.5 °C. It was significantly different between temperatures ≤18 °C, ≥21 °C, and 32.5 °C. The cellular toxin production (CTP, pg·cell(−1)·day(−1)) showed variation with growth phase and temperature, except at 32.5 °C. The average net toxin production (R(tox)) was not affected by temperature. During EG, the average specific toxin production rate (µ(tox)) increased significantly with increase in temperature, reaching a peak of 0.66 ± 0.01 day(−1) at 30 °C, and then decreased. Over the entire growth span, µ(tox) was significantly correlated to µ, and this correlation was most significant at 27 and 30 °C. During EG, µ(tox) was affected by both temperature and growth. This study shows that temperature affects growth and toxin production of this strain of D. caudata during EG. In addition, a positive correlation was found between toxin production and growth.