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Influence of Temperature on Growth and Production of Pectenotoxin-2 by a Monoclonal Culture of Dinophysis caudata
The effects of temperature on growth and production of Lipophilic Toxins (LT) by a monoclonal culture of Dinophysis caudata was studied. The cell density of D. caudata increased significantly with increasing temperature, and was the highest under 27, 30, and 32.5 °C. Temperature affected the average...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4699234/ https://www.ncbi.nlm.nih.gov/pubmed/26633427 http://dx.doi.org/10.3390/md13127061 |
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author | Basti, Leila Uchida, Hajime Matsushima, Ryoji Watanabe, Ryuichi Suzuki, Toshiyuki Yamatogi, Toshifumi Nagai, Satoshi |
author_facet | Basti, Leila Uchida, Hajime Matsushima, Ryoji Watanabe, Ryuichi Suzuki, Toshiyuki Yamatogi, Toshifumi Nagai, Satoshi |
author_sort | Basti, Leila |
collection | PubMed |
description | The effects of temperature on growth and production of Lipophilic Toxins (LT) by a monoclonal culture of Dinophysis caudata was studied. The cell density of D. caudata increased significantly with increasing temperature, and was the highest under 27, 30, and 32.5 °C. Temperature affected the average specific growth rate (µ) during the exponential growth phase (EG), which increased from 15 °C to 30 °C, and then decreased at 32.5 °C. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) revealed that this strain of D. caudata produced only pectenotoxin-2 (PTX-2) whose concentration increased significantly with incubation period, except at 32.5 °C. It was significantly different between temperatures ≤18 °C, ≥21 °C, and 32.5 °C. The cellular toxin production (CTP, pg·cell(−1)·day(−1)) showed variation with growth phase and temperature, except at 32.5 °C. The average net toxin production (R(tox)) was not affected by temperature. During EG, the average specific toxin production rate (µ(tox)) increased significantly with increase in temperature, reaching a peak of 0.66 ± 0.01 day(−1) at 30 °C, and then decreased. Over the entire growth span, µ(tox) was significantly correlated to µ, and this correlation was most significant at 27 and 30 °C. During EG, µ(tox) was affected by both temperature and growth. This study shows that temperature affects growth and toxin production of this strain of D. caudata during EG. In addition, a positive correlation was found between toxin production and growth. |
format | Online Article Text |
id | pubmed-4699234 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-46992342016-01-21 Influence of Temperature on Growth and Production of Pectenotoxin-2 by a Monoclonal Culture of Dinophysis caudata Basti, Leila Uchida, Hajime Matsushima, Ryoji Watanabe, Ryuichi Suzuki, Toshiyuki Yamatogi, Toshifumi Nagai, Satoshi Mar Drugs Article The effects of temperature on growth and production of Lipophilic Toxins (LT) by a monoclonal culture of Dinophysis caudata was studied. The cell density of D. caudata increased significantly with increasing temperature, and was the highest under 27, 30, and 32.5 °C. Temperature affected the average specific growth rate (µ) during the exponential growth phase (EG), which increased from 15 °C to 30 °C, and then decreased at 32.5 °C. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) revealed that this strain of D. caudata produced only pectenotoxin-2 (PTX-2) whose concentration increased significantly with incubation period, except at 32.5 °C. It was significantly different between temperatures ≤18 °C, ≥21 °C, and 32.5 °C. The cellular toxin production (CTP, pg·cell(−1)·day(−1)) showed variation with growth phase and temperature, except at 32.5 °C. The average net toxin production (R(tox)) was not affected by temperature. During EG, the average specific toxin production rate (µ(tox)) increased significantly with increase in temperature, reaching a peak of 0.66 ± 0.01 day(−1) at 30 °C, and then decreased. Over the entire growth span, µ(tox) was significantly correlated to µ, and this correlation was most significant at 27 and 30 °C. During EG, µ(tox) was affected by both temperature and growth. This study shows that temperature affects growth and toxin production of this strain of D. caudata during EG. In addition, a positive correlation was found between toxin production and growth. MDPI 2015-12-03 /pmc/articles/PMC4699234/ /pubmed/26633427 http://dx.doi.org/10.3390/md13127061 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Basti, Leila Uchida, Hajime Matsushima, Ryoji Watanabe, Ryuichi Suzuki, Toshiyuki Yamatogi, Toshifumi Nagai, Satoshi Influence of Temperature on Growth and Production of Pectenotoxin-2 by a Monoclonal Culture of Dinophysis caudata |
title | Influence of Temperature on Growth and Production of Pectenotoxin-2 by a Monoclonal Culture of Dinophysis caudata |
title_full | Influence of Temperature on Growth and Production of Pectenotoxin-2 by a Monoclonal Culture of Dinophysis caudata |
title_fullStr | Influence of Temperature on Growth and Production of Pectenotoxin-2 by a Monoclonal Culture of Dinophysis caudata |
title_full_unstemmed | Influence of Temperature on Growth and Production of Pectenotoxin-2 by a Monoclonal Culture of Dinophysis caudata |
title_short | Influence of Temperature on Growth and Production of Pectenotoxin-2 by a Monoclonal Culture of Dinophysis caudata |
title_sort | influence of temperature on growth and production of pectenotoxin-2 by a monoclonal culture of dinophysis caudata |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4699234/ https://www.ncbi.nlm.nih.gov/pubmed/26633427 http://dx.doi.org/10.3390/md13127061 |
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