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Effect of Ixeris dentata Nakai Extract on Nitric Oxide Production and Prostaglandin E(2) Generation in LPS-stimulated RAW264.7 Cells

Inflammation is the basis of severe acute and chronic diseases. This study investigated the anti-inflammatory property of a crude methanol extract (MeOH-ex) and the solvent fractions of Ixeris dentata Nakai (IDN) in LPS-stimulated murine macrophage-like cell line RAW264.7. Here, we showed that the e...

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Autores principales: Jung, Yu Yeon, Hong, Jin Tae, Han, Sang Bae, Park, Young Hyun, Son, Dong Ju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Association of Immunologists 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4700409/
https://www.ncbi.nlm.nih.gov/pubmed/26770187
http://dx.doi.org/10.4110/in.2015.15.6.325
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author Jung, Yu Yeon
Hong, Jin Tae
Han, Sang Bae
Park, Young Hyun
Son, Dong Ju
author_facet Jung, Yu Yeon
Hong, Jin Tae
Han, Sang Bae
Park, Young Hyun
Son, Dong Ju
author_sort Jung, Yu Yeon
collection PubMed
description Inflammation is the basis of severe acute and chronic diseases. This study investigated the anti-inflammatory property of a crude methanol extract (MeOH-ex) and the solvent fractions of Ixeris dentata Nakai (IDN) in LPS-stimulated murine macrophage-like cell line RAW264.7. Here, we showed that the ethyl acetate fraction (EtOAc-fr) had the most potent inhibitory activity on LPS-induced nitric oxide (NO) production among the tested samples, i.e., IDN MeOH-ex and the three different solvent fractions (chloroform, n-hexane, and EtOAc). We further found that the EtOAc-fr significantly inhibited LPS-induced prostaglandin PGE2 (PGE(2)) generation in RAW264.7 cells. Furthermore, the treatment with EtOAc-fr effectively suppressed the expression of inducible NO synthase (iNOS) and cyclooxygenase 2 (COX-2). These results suggest that the EtOAc-fr of IDN MeOH-ex exhibits an anti-inflammatory activity in vitro by inhibiting LPS-induced NO production and PGE(2) generation via suppression of iNOS and COX-2 expression.
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spelling pubmed-47004092016-01-14 Effect of Ixeris dentata Nakai Extract on Nitric Oxide Production and Prostaglandin E(2) Generation in LPS-stimulated RAW264.7 Cells Jung, Yu Yeon Hong, Jin Tae Han, Sang Bae Park, Young Hyun Son, Dong Ju Immune Netw Brief Communication Inflammation is the basis of severe acute and chronic diseases. This study investigated the anti-inflammatory property of a crude methanol extract (MeOH-ex) and the solvent fractions of Ixeris dentata Nakai (IDN) in LPS-stimulated murine macrophage-like cell line RAW264.7. Here, we showed that the ethyl acetate fraction (EtOAc-fr) had the most potent inhibitory activity on LPS-induced nitric oxide (NO) production among the tested samples, i.e., IDN MeOH-ex and the three different solvent fractions (chloroform, n-hexane, and EtOAc). We further found that the EtOAc-fr significantly inhibited LPS-induced prostaglandin PGE2 (PGE(2)) generation in RAW264.7 cells. Furthermore, the treatment with EtOAc-fr effectively suppressed the expression of inducible NO synthase (iNOS) and cyclooxygenase 2 (COX-2). These results suggest that the EtOAc-fr of IDN MeOH-ex exhibits an anti-inflammatory activity in vitro by inhibiting LPS-induced NO production and PGE(2) generation via suppression of iNOS and COX-2 expression. The Korean Association of Immunologists 2015-12 2015-12-24 /pmc/articles/PMC4700409/ /pubmed/26770187 http://dx.doi.org/10.4110/in.2015.15.6.325 Text en Copyright © 2015 The Korean Association of Immunologists http://creativecommons.org/licenses/by-nc/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Communication
Jung, Yu Yeon
Hong, Jin Tae
Han, Sang Bae
Park, Young Hyun
Son, Dong Ju
Effect of Ixeris dentata Nakai Extract on Nitric Oxide Production and Prostaglandin E(2) Generation in LPS-stimulated RAW264.7 Cells
title Effect of Ixeris dentata Nakai Extract on Nitric Oxide Production and Prostaglandin E(2) Generation in LPS-stimulated RAW264.7 Cells
title_full Effect of Ixeris dentata Nakai Extract on Nitric Oxide Production and Prostaglandin E(2) Generation in LPS-stimulated RAW264.7 Cells
title_fullStr Effect of Ixeris dentata Nakai Extract on Nitric Oxide Production and Prostaglandin E(2) Generation in LPS-stimulated RAW264.7 Cells
title_full_unstemmed Effect of Ixeris dentata Nakai Extract on Nitric Oxide Production and Prostaglandin E(2) Generation in LPS-stimulated RAW264.7 Cells
title_short Effect of Ixeris dentata Nakai Extract on Nitric Oxide Production and Prostaglandin E(2) Generation in LPS-stimulated RAW264.7 Cells
title_sort effect of ixeris dentata nakai extract on nitric oxide production and prostaglandin e(2) generation in lps-stimulated raw264.7 cells
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4700409/
https://www.ncbi.nlm.nih.gov/pubmed/26770187
http://dx.doi.org/10.4110/in.2015.15.6.325
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