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Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824

BACKGROUND: Clostridium acetobutylicum represents a paradigm chassis for the industrial production of the biofuel biobutanol and a focus for metabolic engineering. We have previously developed procedures for the creation of in-frame, marker-less deletion mutants in the pathogen Clostridium difficile...

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Detalles Bibliográficos
Autores principales:	Ehsaan, Muhammad, Kuit, Wouter, Zhang, Ying, Cartman, Stephen T., Heap, John T., Winzer, Klaus, Minton, Nigel P.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2016
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4700727/ https://www.ncbi.nlm.nih.gov/pubmed/26732067 http://dx.doi.org/10.1186/s13068-015-0410-0

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