Cargando…

Serum IGFBP4 concentration decreased in dairy heifers towards day 18 of pregnancy

This study was conducted to determine if the main components of the somatotropic axis change during the early phase of pregnancy in the maternal blood system and whether differences exist on day 18 after pregnancy recognition by the maternal organism. Blood samples of pregnant heifers (Holstein Frie...

Descripción completa

Detalles Bibliográficos
Autores principales: Meyerholz, Marie M, Mense, Kirsten, Lietzau, Michael, Kassens, Ana, Linden, Matthias, Knaack, Hendrike, Wirthgen, Elisa, Hoeflich, Andreas, Raliou, Mariam, Richard, Christophe, Sandra, Olivier, Schuberth, Hans-Joachim, Hoedemaker, Martina, Schmicke, Marion
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4701733/
https://www.ncbi.nlm.nih.gov/pubmed/26243597
http://dx.doi.org/10.4142/jvs.2015.16.4.413
_version_ 1782408520133509120
author Meyerholz, Marie M
Mense, Kirsten
Lietzau, Michael
Kassens, Ana
Linden, Matthias
Knaack, Hendrike
Wirthgen, Elisa
Hoeflich, Andreas
Raliou, Mariam
Richard, Christophe
Sandra, Olivier
Schuberth, Hans-Joachim
Hoedemaker, Martina
Schmicke, Marion
author_facet Meyerholz, Marie M
Mense, Kirsten
Lietzau, Michael
Kassens, Ana
Linden, Matthias
Knaack, Hendrike
Wirthgen, Elisa
Hoeflich, Andreas
Raliou, Mariam
Richard, Christophe
Sandra, Olivier
Schuberth, Hans-Joachim
Hoedemaker, Martina
Schmicke, Marion
author_sort Meyerholz, Marie M
collection PubMed
description This study was conducted to determine if the main components of the somatotropic axis change during the early phase of pregnancy in the maternal blood system and whether differences exist on day 18 after pregnancy recognition by the maternal organism. Blood samples of pregnant heifers (Holstein Friesian; n = 10 after embryo transfer) were obtained on the day of ovulation (day 0), as well as on days 7, 14, 16 and 18 and during pregnant, non-pregnant and negative control cycles. The oncentrations of progesterone (P(4)), oestrogen, growth hormone (GH), insulin-like growth factor-1 and -2 (IGF1, -2) and IGF-binding protein-2, -3 and -4 (IGFBP2, -3, -4) were measured. The mRNA expressions of growth hormone receptor 1A, IGF1, IGF2, IGFBP2, IGFBP3 and IGFBP4 were detected using RT-qPCR in liver biopsy specimens (day 18). In all groups, total serum IGF1 decreased from day 0 to 16. Notably, IGFBP4 maternal blood concentrations were lower during pregnancy than during non-pregnant cycles and synchronized control cycles. It can be speculated that the lower IGFBP4 in maternal blood may result in an increase of free IGF1 for local action. Further studies regarding IGFBP4 concentration and healthy early pregnancy are warranted.
format Online
Article
Text
id pubmed-4701733
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher The Korean Society of Veterinary Science
record_format MEDLINE/PubMed
spelling pubmed-47017332016-01-14 Serum IGFBP4 concentration decreased in dairy heifers towards day 18 of pregnancy Meyerholz, Marie M Mense, Kirsten Lietzau, Michael Kassens, Ana Linden, Matthias Knaack, Hendrike Wirthgen, Elisa Hoeflich, Andreas Raliou, Mariam Richard, Christophe Sandra, Olivier Schuberth, Hans-Joachim Hoedemaker, Martina Schmicke, Marion J Vet Sci Original Article This study was conducted to determine if the main components of the somatotropic axis change during the early phase of pregnancy in the maternal blood system and whether differences exist on day 18 after pregnancy recognition by the maternal organism. Blood samples of pregnant heifers (Holstein Friesian; n = 10 after embryo transfer) were obtained on the day of ovulation (day 0), as well as on days 7, 14, 16 and 18 and during pregnant, non-pregnant and negative control cycles. The oncentrations of progesterone (P(4)), oestrogen, growth hormone (GH), insulin-like growth factor-1 and -2 (IGF1, -2) and IGF-binding protein-2, -3 and -4 (IGFBP2, -3, -4) were measured. The mRNA expressions of growth hormone receptor 1A, IGF1, IGF2, IGFBP2, IGFBP3 and IGFBP4 were detected using RT-qPCR in liver biopsy specimens (day 18). In all groups, total serum IGF1 decreased from day 0 to 16. Notably, IGFBP4 maternal blood concentrations were lower during pregnancy than during non-pregnant cycles and synchronized control cycles. It can be speculated that the lower IGFBP4 in maternal blood may result in an increase of free IGF1 for local action. Further studies regarding IGFBP4 concentration and healthy early pregnancy are warranted. The Korean Society of Veterinary Science 2015-12 2015-12-17 /pmc/articles/PMC4701733/ /pubmed/26243597 http://dx.doi.org/10.4142/jvs.2015.16.4.413 Text en © 2015 The Korean Society of Veterinary Science. http://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Meyerholz, Marie M
Mense, Kirsten
Lietzau, Michael
Kassens, Ana
Linden, Matthias
Knaack, Hendrike
Wirthgen, Elisa
Hoeflich, Andreas
Raliou, Mariam
Richard, Christophe
Sandra, Olivier
Schuberth, Hans-Joachim
Hoedemaker, Martina
Schmicke, Marion
Serum IGFBP4 concentration decreased in dairy heifers towards day 18 of pregnancy
title Serum IGFBP4 concentration decreased in dairy heifers towards day 18 of pregnancy
title_full Serum IGFBP4 concentration decreased in dairy heifers towards day 18 of pregnancy
title_fullStr Serum IGFBP4 concentration decreased in dairy heifers towards day 18 of pregnancy
title_full_unstemmed Serum IGFBP4 concentration decreased in dairy heifers towards day 18 of pregnancy
title_short Serum IGFBP4 concentration decreased in dairy heifers towards day 18 of pregnancy
title_sort serum igfbp4 concentration decreased in dairy heifers towards day 18 of pregnancy
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4701733/
https://www.ncbi.nlm.nih.gov/pubmed/26243597
http://dx.doi.org/10.4142/jvs.2015.16.4.413
work_keys_str_mv AT meyerholzmariem serumigfbp4concentrationdecreasedindairyheiferstowardsday18ofpregnancy
AT mensekirsten serumigfbp4concentrationdecreasedindairyheiferstowardsday18ofpregnancy
AT lietzaumichael serumigfbp4concentrationdecreasedindairyheiferstowardsday18ofpregnancy
AT kassensana serumigfbp4concentrationdecreasedindairyheiferstowardsday18ofpregnancy
AT lindenmatthias serumigfbp4concentrationdecreasedindairyheiferstowardsday18ofpregnancy
AT knaackhendrike serumigfbp4concentrationdecreasedindairyheiferstowardsday18ofpregnancy
AT wirthgenelisa serumigfbp4concentrationdecreasedindairyheiferstowardsday18ofpregnancy
AT hoeflichandreas serumigfbp4concentrationdecreasedindairyheiferstowardsday18ofpregnancy
AT ralioumariam serumigfbp4concentrationdecreasedindairyheiferstowardsday18ofpregnancy
AT richardchristophe serumigfbp4concentrationdecreasedindairyheiferstowardsday18ofpregnancy
AT sandraolivier serumigfbp4concentrationdecreasedindairyheiferstowardsday18ofpregnancy
AT schuberthhansjoachim serumigfbp4concentrationdecreasedindairyheiferstowardsday18ofpregnancy
AT hoedemakermartina serumigfbp4concentrationdecreasedindairyheiferstowardsday18ofpregnancy
AT schmickemarion serumigfbp4concentrationdecreasedindairyheiferstowardsday18ofpregnancy