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Serum IGFBP4 concentration decreased in dairy heifers towards day 18 of pregnancy
This study was conducted to determine if the main components of the somatotropic axis change during the early phase of pregnancy in the maternal blood system and whether differences exist on day 18 after pregnancy recognition by the maternal organism. Blood samples of pregnant heifers (Holstein Frie...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Veterinary Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4701733/ https://www.ncbi.nlm.nih.gov/pubmed/26243597 http://dx.doi.org/10.4142/jvs.2015.16.4.413 |
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author | Meyerholz, Marie M Mense, Kirsten Lietzau, Michael Kassens, Ana Linden, Matthias Knaack, Hendrike Wirthgen, Elisa Hoeflich, Andreas Raliou, Mariam Richard, Christophe Sandra, Olivier Schuberth, Hans-Joachim Hoedemaker, Martina Schmicke, Marion |
author_facet | Meyerholz, Marie M Mense, Kirsten Lietzau, Michael Kassens, Ana Linden, Matthias Knaack, Hendrike Wirthgen, Elisa Hoeflich, Andreas Raliou, Mariam Richard, Christophe Sandra, Olivier Schuberth, Hans-Joachim Hoedemaker, Martina Schmicke, Marion |
author_sort | Meyerholz, Marie M |
collection | PubMed |
description | This study was conducted to determine if the main components of the somatotropic axis change during the early phase of pregnancy in the maternal blood system and whether differences exist on day 18 after pregnancy recognition by the maternal organism. Blood samples of pregnant heifers (Holstein Friesian; n = 10 after embryo transfer) were obtained on the day of ovulation (day 0), as well as on days 7, 14, 16 and 18 and during pregnant, non-pregnant and negative control cycles. The oncentrations of progesterone (P(4)), oestrogen, growth hormone (GH), insulin-like growth factor-1 and -2 (IGF1, -2) and IGF-binding protein-2, -3 and -4 (IGFBP2, -3, -4) were measured. The mRNA expressions of growth hormone receptor 1A, IGF1, IGF2, IGFBP2, IGFBP3 and IGFBP4 were detected using RT-qPCR in liver biopsy specimens (day 18). In all groups, total serum IGF1 decreased from day 0 to 16. Notably, IGFBP4 maternal blood concentrations were lower during pregnancy than during non-pregnant cycles and synchronized control cycles. It can be speculated that the lower IGFBP4 in maternal blood may result in an increase of free IGF1 for local action. Further studies regarding IGFBP4 concentration and healthy early pregnancy are warranted. |
format | Online Article Text |
id | pubmed-4701733 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The Korean Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-47017332016-01-14 Serum IGFBP4 concentration decreased in dairy heifers towards day 18 of pregnancy Meyerholz, Marie M Mense, Kirsten Lietzau, Michael Kassens, Ana Linden, Matthias Knaack, Hendrike Wirthgen, Elisa Hoeflich, Andreas Raliou, Mariam Richard, Christophe Sandra, Olivier Schuberth, Hans-Joachim Hoedemaker, Martina Schmicke, Marion J Vet Sci Original Article This study was conducted to determine if the main components of the somatotropic axis change during the early phase of pregnancy in the maternal blood system and whether differences exist on day 18 after pregnancy recognition by the maternal organism. Blood samples of pregnant heifers (Holstein Friesian; n = 10 after embryo transfer) were obtained on the day of ovulation (day 0), as well as on days 7, 14, 16 and 18 and during pregnant, non-pregnant and negative control cycles. The oncentrations of progesterone (P(4)), oestrogen, growth hormone (GH), insulin-like growth factor-1 and -2 (IGF1, -2) and IGF-binding protein-2, -3 and -4 (IGFBP2, -3, -4) were measured. The mRNA expressions of growth hormone receptor 1A, IGF1, IGF2, IGFBP2, IGFBP3 and IGFBP4 were detected using RT-qPCR in liver biopsy specimens (day 18). In all groups, total serum IGF1 decreased from day 0 to 16. Notably, IGFBP4 maternal blood concentrations were lower during pregnancy than during non-pregnant cycles and synchronized control cycles. It can be speculated that the lower IGFBP4 in maternal blood may result in an increase of free IGF1 for local action. Further studies regarding IGFBP4 concentration and healthy early pregnancy are warranted. The Korean Society of Veterinary Science 2015-12 2015-12-17 /pmc/articles/PMC4701733/ /pubmed/26243597 http://dx.doi.org/10.4142/jvs.2015.16.4.413 Text en © 2015 The Korean Society of Veterinary Science. http://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Meyerholz, Marie M Mense, Kirsten Lietzau, Michael Kassens, Ana Linden, Matthias Knaack, Hendrike Wirthgen, Elisa Hoeflich, Andreas Raliou, Mariam Richard, Christophe Sandra, Olivier Schuberth, Hans-Joachim Hoedemaker, Martina Schmicke, Marion Serum IGFBP4 concentration decreased in dairy heifers towards day 18 of pregnancy |
title | Serum IGFBP4 concentration decreased in dairy heifers towards day 18 of pregnancy |
title_full | Serum IGFBP4 concentration decreased in dairy heifers towards day 18 of pregnancy |
title_fullStr | Serum IGFBP4 concentration decreased in dairy heifers towards day 18 of pregnancy |
title_full_unstemmed | Serum IGFBP4 concentration decreased in dairy heifers towards day 18 of pregnancy |
title_short | Serum IGFBP4 concentration decreased in dairy heifers towards day 18 of pregnancy |
title_sort | serum igfbp4 concentration decreased in dairy heifers towards day 18 of pregnancy |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4701733/ https://www.ncbi.nlm.nih.gov/pubmed/26243597 http://dx.doi.org/10.4142/jvs.2015.16.4.413 |
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