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Intravital imaging of Ca(2+) signals in lymphocytes of Ca(2+) biosensor transgenic mice: indication of autoimmune diseases before the pathological onset
Calcium ion (Ca(2+)) signaling is a typical phenomenon mediated through immune receptors, such as the B-cell antigen receptor (BCR), and it is important for their biological activities. To analyze the signaling of immune receptors together with their in vivo dynamics, we generated stable transgenic...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4702216/ https://www.ncbi.nlm.nih.gov/pubmed/26732477 http://dx.doi.org/10.1038/srep18738 |
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author | Yoshikawa, Soichiro Usami, Takako Kikuta, Junichi Ishii, Masaru Sasano, Tetsuo Sugiyama, Koji Furukawa, Tetsushi Nakasho, Eiji Takayanagi, Hiroshi Tedder, Thomas F. Karasuyama, Hajime Miyawaki, Atsushi Adachi, Takahiro |
author_facet | Yoshikawa, Soichiro Usami, Takako Kikuta, Junichi Ishii, Masaru Sasano, Tetsuo Sugiyama, Koji Furukawa, Tetsushi Nakasho, Eiji Takayanagi, Hiroshi Tedder, Thomas F. Karasuyama, Hajime Miyawaki, Atsushi Adachi, Takahiro |
author_sort | Yoshikawa, Soichiro |
collection | PubMed |
description | Calcium ion (Ca(2+)) signaling is a typical phenomenon mediated through immune receptors, such as the B-cell antigen receptor (BCR), and it is important for their biological activities. To analyze the signaling of immune receptors together with their in vivo dynamics, we generated stable transgenic mice with the Föster/fluorescence resonance energy transfer (FRET)-based Ca(2+) indicator yellow cameleon 3.60 (YC3.60), based on the Cre/loxP system (YC3.60(flox)). We successfully obtained mice with specific YC3.60 expression in immune or nerve cells as well as mice with ubiquitous expression of this indicator. We established five-dimensional (5D) (x, y, z, time, and Ca(2+)) intravital imaging of lymphoid tissues, including the bone marrow. Furthermore, in autoimmune-prone models, the CD22(−/−) and C57BL/6- lymphoproliferation (lpr)/lpr mouse, Ca(2+) fluxes were augmented, although they did not induce autoimmune disease. Intravital imaging of Ca(2+) signals in lymphocytes may improve assessment of the risk of autoimmune diseases in model animals. |
format | Online Article Text |
id | pubmed-4702216 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-47022162016-01-19 Intravital imaging of Ca(2+) signals in lymphocytes of Ca(2+) biosensor transgenic mice: indication of autoimmune diseases before the pathological onset Yoshikawa, Soichiro Usami, Takako Kikuta, Junichi Ishii, Masaru Sasano, Tetsuo Sugiyama, Koji Furukawa, Tetsushi Nakasho, Eiji Takayanagi, Hiroshi Tedder, Thomas F. Karasuyama, Hajime Miyawaki, Atsushi Adachi, Takahiro Sci Rep Article Calcium ion (Ca(2+)) signaling is a typical phenomenon mediated through immune receptors, such as the B-cell antigen receptor (BCR), and it is important for their biological activities. To analyze the signaling of immune receptors together with their in vivo dynamics, we generated stable transgenic mice with the Föster/fluorescence resonance energy transfer (FRET)-based Ca(2+) indicator yellow cameleon 3.60 (YC3.60), based on the Cre/loxP system (YC3.60(flox)). We successfully obtained mice with specific YC3.60 expression in immune or nerve cells as well as mice with ubiquitous expression of this indicator. We established five-dimensional (5D) (x, y, z, time, and Ca(2+)) intravital imaging of lymphoid tissues, including the bone marrow. Furthermore, in autoimmune-prone models, the CD22(−/−) and C57BL/6- lymphoproliferation (lpr)/lpr mouse, Ca(2+) fluxes were augmented, although they did not induce autoimmune disease. Intravital imaging of Ca(2+) signals in lymphocytes may improve assessment of the risk of autoimmune diseases in model animals. Nature Publishing Group 2016-01-06 /pmc/articles/PMC4702216/ /pubmed/26732477 http://dx.doi.org/10.1038/srep18738 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Yoshikawa, Soichiro Usami, Takako Kikuta, Junichi Ishii, Masaru Sasano, Tetsuo Sugiyama, Koji Furukawa, Tetsushi Nakasho, Eiji Takayanagi, Hiroshi Tedder, Thomas F. Karasuyama, Hajime Miyawaki, Atsushi Adachi, Takahiro Intravital imaging of Ca(2+) signals in lymphocytes of Ca(2+) biosensor transgenic mice: indication of autoimmune diseases before the pathological onset |
title | Intravital imaging of Ca(2+) signals in lymphocytes of Ca(2+) biosensor transgenic mice: indication of autoimmune diseases before the pathological onset |
title_full | Intravital imaging of Ca(2+) signals in lymphocytes of Ca(2+) biosensor transgenic mice: indication of autoimmune diseases before the pathological onset |
title_fullStr | Intravital imaging of Ca(2+) signals in lymphocytes of Ca(2+) biosensor transgenic mice: indication of autoimmune diseases before the pathological onset |
title_full_unstemmed | Intravital imaging of Ca(2+) signals in lymphocytes of Ca(2+) biosensor transgenic mice: indication of autoimmune diseases before the pathological onset |
title_short | Intravital imaging of Ca(2+) signals in lymphocytes of Ca(2+) biosensor transgenic mice: indication of autoimmune diseases before the pathological onset |
title_sort | intravital imaging of ca(2+) signals in lymphocytes of ca(2+) biosensor transgenic mice: indication of autoimmune diseases before the pathological onset |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4702216/ https://www.ncbi.nlm.nih.gov/pubmed/26732477 http://dx.doi.org/10.1038/srep18738 |
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