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MicroRNA-26a suppresses epithelial-mesenchymal transition in human hepatocellular carcinoma by repressing enhancer of zeste homolog 2

BACKGROUND: Our previous study reported that microRNA-26a (miR-26a) inhibited tumor progression by inhibiting tumor angiogenesis and intratumoral macrophage infiltration in hepatocellular carcinoma (HCC). The direct roles of miR-26a on tumor cell invasion remain poorly understood. In this study, we...

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Autores principales: Ma, De-Ning, Chai, Zong-Tao, Zhu, Xiao-Dong, Zhang, Ning, Zhan, Di-Hua, Ye, Bo-Gen, Wang, Cheng-Hao, Qin, Cheng-Dong, Zhao, Yi-Ming, Zhu, Wei-Ping, Cao, Man-Qing, Gao, Dong-Mei, Sun, Hui-Chuan, Tang, Zhao-You
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4702409/
https://www.ncbi.nlm.nih.gov/pubmed/26733151
http://dx.doi.org/10.1186/s13045-015-0229-y
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author Ma, De-Ning
Chai, Zong-Tao
Zhu, Xiao-Dong
Zhang, Ning
Zhan, Di-Hua
Ye, Bo-Gen
Wang, Cheng-Hao
Qin, Cheng-Dong
Zhao, Yi-Ming
Zhu, Wei-Ping
Cao, Man-Qing
Gao, Dong-Mei
Sun, Hui-Chuan
Tang, Zhao-You
author_facet Ma, De-Ning
Chai, Zong-Tao
Zhu, Xiao-Dong
Zhang, Ning
Zhan, Di-Hua
Ye, Bo-Gen
Wang, Cheng-Hao
Qin, Cheng-Dong
Zhao, Yi-Ming
Zhu, Wei-Ping
Cao, Man-Qing
Gao, Dong-Mei
Sun, Hui-Chuan
Tang, Zhao-You
author_sort Ma, De-Ning
collection PubMed
description BACKGROUND: Our previous study reported that microRNA-26a (miR-26a) inhibited tumor progression by inhibiting tumor angiogenesis and intratumoral macrophage infiltration in hepatocellular carcinoma (HCC). The direct roles of miR-26a on tumor cell invasion remain poorly understood. In this study, we aim to explore the mechanism of miR-26a in modulating epithelial-mesenchymal transition (EMT) in HCC. METHODS: In vitro cell morphology and cell migration were compared between the hepatoma cell lines HCCLM3 and HepG2, which were established in the previous study. Overexpression and down-regulation of miR-26a were induced in these cell lines, and Western blot and immunofluorescence assays were used to detect the expression of EMT markers. Xenograft nude mouse models were used to observe tumor growth and pulmonary metastasis. Immunohistochemical assays were conducted to study the relationships between miR-26a expression and enhancer of zeste homolog 2 (EZH2) and E-cadherin expression in human HCC samples. RESULTS: Down-regulation of miR-26a in HCCLM3 and HepG2 cells resulted in an EMT-like cell morphology and high motility in vitro and increased in tumor growth and pulmonary metastasis in vivo. Through down-regulation of EZH2 expression and up-regulation of E-cadherin expression, miR-26a inhibited the EMT process in vitro and in vivo. Luciferase reporter assay showed that miR-26a directly interacted with EZH2 messenger RNA (mRNA). Furthermore, the expression of miR-26a was positively correlated with E-cadherin expression and inversely correlated with EZH2 expression in human HCC tissue. CONCLUSIONS: miR-26a inhibited the EMT process in HCC by down-regulating EZH2 expression.
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spelling pubmed-47024092016-01-07 MicroRNA-26a suppresses epithelial-mesenchymal transition in human hepatocellular carcinoma by repressing enhancer of zeste homolog 2 Ma, De-Ning Chai, Zong-Tao Zhu, Xiao-Dong Zhang, Ning Zhan, Di-Hua Ye, Bo-Gen Wang, Cheng-Hao Qin, Cheng-Dong Zhao, Yi-Ming Zhu, Wei-Ping Cao, Man-Qing Gao, Dong-Mei Sun, Hui-Chuan Tang, Zhao-You J Hematol Oncol Research BACKGROUND: Our previous study reported that microRNA-26a (miR-26a) inhibited tumor progression by inhibiting tumor angiogenesis and intratumoral macrophage infiltration in hepatocellular carcinoma (HCC). The direct roles of miR-26a on tumor cell invasion remain poorly understood. In this study, we aim to explore the mechanism of miR-26a in modulating epithelial-mesenchymal transition (EMT) in HCC. METHODS: In vitro cell morphology and cell migration were compared between the hepatoma cell lines HCCLM3 and HepG2, which were established in the previous study. Overexpression and down-regulation of miR-26a were induced in these cell lines, and Western blot and immunofluorescence assays were used to detect the expression of EMT markers. Xenograft nude mouse models were used to observe tumor growth and pulmonary metastasis. Immunohistochemical assays were conducted to study the relationships between miR-26a expression and enhancer of zeste homolog 2 (EZH2) and E-cadherin expression in human HCC samples. RESULTS: Down-regulation of miR-26a in HCCLM3 and HepG2 cells resulted in an EMT-like cell morphology and high motility in vitro and increased in tumor growth and pulmonary metastasis in vivo. Through down-regulation of EZH2 expression and up-regulation of E-cadherin expression, miR-26a inhibited the EMT process in vitro and in vivo. Luciferase reporter assay showed that miR-26a directly interacted with EZH2 messenger RNA (mRNA). Furthermore, the expression of miR-26a was positively correlated with E-cadherin expression and inversely correlated with EZH2 expression in human HCC tissue. CONCLUSIONS: miR-26a inhibited the EMT process in HCC by down-regulating EZH2 expression. BioMed Central 2016-01-06 /pmc/articles/PMC4702409/ /pubmed/26733151 http://dx.doi.org/10.1186/s13045-015-0229-y Text en © Ma et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Ma, De-Ning
Chai, Zong-Tao
Zhu, Xiao-Dong
Zhang, Ning
Zhan, Di-Hua
Ye, Bo-Gen
Wang, Cheng-Hao
Qin, Cheng-Dong
Zhao, Yi-Ming
Zhu, Wei-Ping
Cao, Man-Qing
Gao, Dong-Mei
Sun, Hui-Chuan
Tang, Zhao-You
MicroRNA-26a suppresses epithelial-mesenchymal transition in human hepatocellular carcinoma by repressing enhancer of zeste homolog 2
title MicroRNA-26a suppresses epithelial-mesenchymal transition in human hepatocellular carcinoma by repressing enhancer of zeste homolog 2
title_full MicroRNA-26a suppresses epithelial-mesenchymal transition in human hepatocellular carcinoma by repressing enhancer of zeste homolog 2
title_fullStr MicroRNA-26a suppresses epithelial-mesenchymal transition in human hepatocellular carcinoma by repressing enhancer of zeste homolog 2
title_full_unstemmed MicroRNA-26a suppresses epithelial-mesenchymal transition in human hepatocellular carcinoma by repressing enhancer of zeste homolog 2
title_short MicroRNA-26a suppresses epithelial-mesenchymal transition in human hepatocellular carcinoma by repressing enhancer of zeste homolog 2
title_sort microrna-26a suppresses epithelial-mesenchymal transition in human hepatocellular carcinoma by repressing enhancer of zeste homolog 2
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4702409/
https://www.ncbi.nlm.nih.gov/pubmed/26733151
http://dx.doi.org/10.1186/s13045-015-0229-y
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