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Heterogeneity of the gut microbiome in mice: guidelines for optimizing experimental design
Targeted manipulation of the gut flora is increasingly being recognized as a means to improve human health. Yet, the temporal dynamics and intra- and interindividual heterogeneity of the microbiome represent experimental limitations, especially in human cross-sectional studies. Therefore, rodent mod...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4703068/ https://www.ncbi.nlm.nih.gov/pubmed/26323480 http://dx.doi.org/10.1093/femsre/fuv036 |
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author | Laukens, Debby Brinkman, Brigitta M. Raes, Jeroen De Vos, Martine Vandenabeele, Peter |
author_facet | Laukens, Debby Brinkman, Brigitta M. Raes, Jeroen De Vos, Martine Vandenabeele, Peter |
author_sort | Laukens, Debby |
collection | PubMed |
description | Targeted manipulation of the gut flora is increasingly being recognized as a means to improve human health. Yet, the temporal dynamics and intra- and interindividual heterogeneity of the microbiome represent experimental limitations, especially in human cross-sectional studies. Therefore, rodent models represent an invaluable tool to study the host–microbiota interface. Progress in technical and computational tools to investigate the composition and function of the microbiome has opened a new era of research and we gradually begin to understand the parameters that influence variation of host-associated microbial communities. To isolate true effects from confounding factors, it is essential to include such parameters in model intervention studies. Also, explicit journal instructions to include essential information on animal experiments are mandatory. The purpose of this review is to summarize the factors that influence microbiota composition in mice and to provide guidelines to improve the reproducibility of animal experiments. |
format | Online Article Text |
id | pubmed-4703068 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-47030682016-01-07 Heterogeneity of the gut microbiome in mice: guidelines for optimizing experimental design Laukens, Debby Brinkman, Brigitta M. Raes, Jeroen De Vos, Martine Vandenabeele, Peter FEMS Microbiol Rev Review Article Targeted manipulation of the gut flora is increasingly being recognized as a means to improve human health. Yet, the temporal dynamics and intra- and interindividual heterogeneity of the microbiome represent experimental limitations, especially in human cross-sectional studies. Therefore, rodent models represent an invaluable tool to study the host–microbiota interface. Progress in technical and computational tools to investigate the composition and function of the microbiome has opened a new era of research and we gradually begin to understand the parameters that influence variation of host-associated microbial communities. To isolate true effects from confounding factors, it is essential to include such parameters in model intervention studies. Also, explicit journal instructions to include essential information on animal experiments are mandatory. The purpose of this review is to summarize the factors that influence microbiota composition in mice and to provide guidelines to improve the reproducibility of animal experiments. Oxford University Press 2015-08-30 2016-01 /pmc/articles/PMC4703068/ /pubmed/26323480 http://dx.doi.org/10.1093/femsre/fuv036 Text en © FEMS 2015. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in anymedium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Review Article Laukens, Debby Brinkman, Brigitta M. Raes, Jeroen De Vos, Martine Vandenabeele, Peter Heterogeneity of the gut microbiome in mice: guidelines for optimizing experimental design |
title | Heterogeneity of the gut microbiome in mice: guidelines for optimizing experimental design |
title_full | Heterogeneity of the gut microbiome in mice: guidelines for optimizing experimental design |
title_fullStr | Heterogeneity of the gut microbiome in mice: guidelines for optimizing experimental design |
title_full_unstemmed | Heterogeneity of the gut microbiome in mice: guidelines for optimizing experimental design |
title_short | Heterogeneity of the gut microbiome in mice: guidelines for optimizing experimental design |
title_sort | heterogeneity of the gut microbiome in mice: guidelines for optimizing experimental design |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4703068/ https://www.ncbi.nlm.nih.gov/pubmed/26323480 http://dx.doi.org/10.1093/femsre/fuv036 |
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