Visualisation of newly synthesised collagen in vitro and in vivo

Identifying collagen produced de novo by cells in a background of purified collagenous biomaterials poses a major problem in for example the evaluation of tissue-engineered constructs and cell biological studies to tumor dissemination. We have developed a universal strategy to detect and localize ne...

Descripción completa

Detalles Bibliográficos
Autores principales: Oostendorp, Corien, Uijtdewilligen, Peter J.E., Versteeg, Elly M., Hafmans, Theo G., van den Bogaard, Ellen H., de Jonge, Paul K.J.D., Pirayesh, Ali, Von den Hoff, Johannes W., Reichmann, Ernst, Daamen, Willeke F., van Kuppevelt, Toin H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4704054/
https://www.ncbi.nlm.nih.gov/pubmed/26738984
http://dx.doi.org/10.1038/srep18780
Descripción
Sumario:Identifying collagen produced de novo by cells in a background of purified collagenous biomaterials poses a major problem in for example the evaluation of tissue-engineered constructs and cell biological studies to tumor dissemination. We have developed a universal strategy to detect and localize newly deposited collagen based on its inherent association with dermatan sulfate. The method is applicable irrespective of host species and collagen source.

Ejemplares similares