Visualisation of newly synthesised collagen in vitro and in vivo

Identifying collagen produced de novo by cells in a background of purified collagenous biomaterials poses a major problem in for example the evaluation of tissue-engineered constructs and cell biological studies to tumor dissemination. We have developed a universal strategy to detect and localize ne...

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Autores principales: Oostendorp, Corien, Uijtdewilligen, Peter J.E., Versteeg, Elly M., Hafmans, Theo G., van den Bogaard, Ellen H., de Jonge, Paul K.J.D., Pirayesh, Ali, Von den Hoff, Johannes W., Reichmann, Ernst, Daamen, Willeke F., van Kuppevelt, Toin H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4704054/
https://www.ncbi.nlm.nih.gov/pubmed/26738984
http://dx.doi.org/10.1038/srep18780
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author Oostendorp, Corien
Uijtdewilligen, Peter J.E.
Versteeg, Elly M.
Hafmans, Theo G.
van den Bogaard, Ellen H.
de Jonge, Paul K.J.D.
Pirayesh, Ali
Von den Hoff, Johannes W.
Reichmann, Ernst
Daamen, Willeke F.
van Kuppevelt, Toin H.
author_facet Oostendorp, Corien
Uijtdewilligen, Peter J.E.
Versteeg, Elly M.
Hafmans, Theo G.
van den Bogaard, Ellen H.
de Jonge, Paul K.J.D.
Pirayesh, Ali
Von den Hoff, Johannes W.
Reichmann, Ernst
Daamen, Willeke F.
van Kuppevelt, Toin H.
author_sort Oostendorp, Corien
collection PubMed
description Identifying collagen produced de novo by cells in a background of purified collagenous biomaterials poses a major problem in for example the evaluation of tissue-engineered constructs and cell biological studies to tumor dissemination. We have developed a universal strategy to detect and localize newly deposited collagen based on its inherent association with dermatan sulfate. The method is applicable irrespective of host species and collagen source.
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spelling pubmed-47040542016-01-19 Visualisation of newly synthesised collagen in vitro and in vivo Oostendorp, Corien Uijtdewilligen, Peter J.E. Versteeg, Elly M. Hafmans, Theo G. van den Bogaard, Ellen H. de Jonge, Paul K.J.D. Pirayesh, Ali Von den Hoff, Johannes W. Reichmann, Ernst Daamen, Willeke F. van Kuppevelt, Toin H. Sci Rep Article Identifying collagen produced de novo by cells in a background of purified collagenous biomaterials poses a major problem in for example the evaluation of tissue-engineered constructs and cell biological studies to tumor dissemination. We have developed a universal strategy to detect and localize newly deposited collagen based on its inherent association with dermatan sulfate. The method is applicable irrespective of host species and collagen source. Nature Publishing Group 2016-01-07 /pmc/articles/PMC4704054/ /pubmed/26738984 http://dx.doi.org/10.1038/srep18780 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Oostendorp, Corien
Uijtdewilligen, Peter J.E.
Versteeg, Elly M.
Hafmans, Theo G.
van den Bogaard, Ellen H.
de Jonge, Paul K.J.D.
Pirayesh, Ali
Von den Hoff, Johannes W.
Reichmann, Ernst
Daamen, Willeke F.
van Kuppevelt, Toin H.
Visualisation of newly synthesised collagen in vitro and in vivo
title Visualisation of newly synthesised collagen in vitro and in vivo
title_full Visualisation of newly synthesised collagen in vitro and in vivo
title_fullStr Visualisation of newly synthesised collagen in vitro and in vivo
title_full_unstemmed Visualisation of newly synthesised collagen in vitro and in vivo
title_short Visualisation of newly synthesised collagen in vitro and in vivo
title_sort visualisation of newly synthesised collagen in vitro and in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4704054/
https://www.ncbi.nlm.nih.gov/pubmed/26738984
http://dx.doi.org/10.1038/srep18780
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