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Diagnosis of an imported Plasmodium ovale wallikeri infection in Malaysia

Plasmodium ovale is rare and not exactly known to be autochthonous in Malaysia. There are two distinct forms of the parasite, namely P. ovale curtisi (classic form) and P. ovale wallikeri (variant form). Here, the first sequence confirmed case of an imported P. ovale wallikeri infection in Malaysia...

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Autores principales: Liew, Jonathan Wee Kent, Mahmud, Rohela, Tan, Lian Huat, Lau, Yee Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4704402/
https://www.ncbi.nlm.nih.gov/pubmed/26738724
http://dx.doi.org/10.1186/s12936-015-1070-z
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author Liew, Jonathan Wee Kent
Mahmud, Rohela
Tan, Lian Huat
Lau, Yee Ling
author_facet Liew, Jonathan Wee Kent
Mahmud, Rohela
Tan, Lian Huat
Lau, Yee Ling
author_sort Liew, Jonathan Wee Kent
collection PubMed
description Plasmodium ovale is rare and not exactly known to be autochthonous in Malaysia. There are two distinct forms of the parasite, namely P. ovale curtisi (classic form) and P. ovale wallikeri (variant form). Here, the first sequence confirmed case of an imported P. ovale wallikeri infection in Malaysia is presented. Microscopy found Plasmodium parasites with morphology similar to P. ovale or Plasmodium vivax in the blood films. Further confirmation using polymerase chain reaction (PCR) targeting the small-subunit rRNA gene of the parasite was unsuccessful. Genus-specific PCR was then performed and the product was sequenced and analysed. Sequence analyses confirmed the aetiological agent as P. ovale wallikeri. New species-specific primers (rOVA1v and rOVA2v) were employed and P. ovale wallikeri was finally confirmed. The findings highlight the need to look out for imported malaria infections in Malaysia and the importance of a constantly updated and validated diagnostic technique.
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spelling pubmed-47044022016-01-08 Diagnosis of an imported Plasmodium ovale wallikeri infection in Malaysia Liew, Jonathan Wee Kent Mahmud, Rohela Tan, Lian Huat Lau, Yee Ling Malar J Case Report Plasmodium ovale is rare and not exactly known to be autochthonous in Malaysia. There are two distinct forms of the parasite, namely P. ovale curtisi (classic form) and P. ovale wallikeri (variant form). Here, the first sequence confirmed case of an imported P. ovale wallikeri infection in Malaysia is presented. Microscopy found Plasmodium parasites with morphology similar to P. ovale or Plasmodium vivax in the blood films. Further confirmation using polymerase chain reaction (PCR) targeting the small-subunit rRNA gene of the parasite was unsuccessful. Genus-specific PCR was then performed and the product was sequenced and analysed. Sequence analyses confirmed the aetiological agent as P. ovale wallikeri. New species-specific primers (rOVA1v and rOVA2v) were employed and P. ovale wallikeri was finally confirmed. The findings highlight the need to look out for imported malaria infections in Malaysia and the importance of a constantly updated and validated diagnostic technique. BioMed Central 2016-01-06 /pmc/articles/PMC4704402/ /pubmed/26738724 http://dx.doi.org/10.1186/s12936-015-1070-z Text en © Liew at al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Case Report
Liew, Jonathan Wee Kent
Mahmud, Rohela
Tan, Lian Huat
Lau, Yee Ling
Diagnosis of an imported Plasmodium ovale wallikeri infection in Malaysia
title Diagnosis of an imported Plasmodium ovale wallikeri infection in Malaysia
title_full Diagnosis of an imported Plasmodium ovale wallikeri infection in Malaysia
title_fullStr Diagnosis of an imported Plasmodium ovale wallikeri infection in Malaysia
title_full_unstemmed Diagnosis of an imported Plasmodium ovale wallikeri infection in Malaysia
title_short Diagnosis of an imported Plasmodium ovale wallikeri infection in Malaysia
title_sort diagnosis of an imported plasmodium ovale wallikeri infection in malaysia
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4704402/
https://www.ncbi.nlm.nih.gov/pubmed/26738724
http://dx.doi.org/10.1186/s12936-015-1070-z
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