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Biophysicochemical characterization of Pyocin SA189 produced by Pseudomonas aeruginosa SA189
Pseudomonas aeruginosa, in spite of being a ubiquitous organism (as it is found in soil, water, and humans), is also an opportunistic pathogen. In order to maintain its diversity in the community, it produces various toxic proteins, known as, bacteriocins. In the present study, pyocin SA189, which i...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Microbiologia
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4704615/ https://www.ncbi.nlm.nih.gov/pubmed/26691474 http://dx.doi.org/10.1590/S1517-838246420140737 |
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author | Naz, Sehar Afshan Jabeen, Nusrat Sohail, Muhammad Rasool, Sheikh Ajaz |
author_facet | Naz, Sehar Afshan Jabeen, Nusrat Sohail, Muhammad Rasool, Sheikh Ajaz |
author_sort | Naz, Sehar Afshan |
collection | PubMed |
description | Pseudomonas aeruginosa, in spite of being a ubiquitous organism (as it is found in soil, water, and humans), is also an opportunistic pathogen. In order to maintain its diversity in the community, it produces various toxic proteins, known as, bacteriocins. In the present study, pyocin SA189, which is a bacteriocin produced by P. aeruginosa SA189 (isolated from a clinical sample) was characterized. P. aeruginosa SA189, as identified by the conventional and 16S rRNA gene amplification, produced pyocin SA189 of molecular weight of 66 k Da. The pyocin showed antimicrobial activity against several clinically relevant Gram-positive and Gram-negative bacteria and was substantially stable for wide ranges of temperature and pH. Furthermore, the pyocin also retained its biological activity upon treatment with metal ions, organic solvents, and various proteolytic and lipolytic enzymes. The data from the growth kinetics indicated that the maximum bacteriocin production occurred in the late log phase. Overall, our results signify the potential of pyocin SA189 as a bio-control agent. |
format | Online Article Text |
id | pubmed-4704615 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Sociedade Brasileira de Microbiologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-47046152016-01-14 Biophysicochemical characterization of Pyocin SA189 produced by Pseudomonas aeruginosa SA189 Naz, Sehar Afshan Jabeen, Nusrat Sohail, Muhammad Rasool, Sheikh Ajaz Braz J Microbiol Medical Microbiology Pseudomonas aeruginosa, in spite of being a ubiquitous organism (as it is found in soil, water, and humans), is also an opportunistic pathogen. In order to maintain its diversity in the community, it produces various toxic proteins, known as, bacteriocins. In the present study, pyocin SA189, which is a bacteriocin produced by P. aeruginosa SA189 (isolated from a clinical sample) was characterized. P. aeruginosa SA189, as identified by the conventional and 16S rRNA gene amplification, produced pyocin SA189 of molecular weight of 66 k Da. The pyocin showed antimicrobial activity against several clinically relevant Gram-positive and Gram-negative bacteria and was substantially stable for wide ranges of temperature and pH. Furthermore, the pyocin also retained its biological activity upon treatment with metal ions, organic solvents, and various proteolytic and lipolytic enzymes. The data from the growth kinetics indicated that the maximum bacteriocin production occurred in the late log phase. Overall, our results signify the potential of pyocin SA189 as a bio-control agent. Sociedade Brasileira de Microbiologia 2015-12-01 /pmc/articles/PMC4704615/ /pubmed/26691474 http://dx.doi.org/10.1590/S1517-838246420140737 Text en Copyright © 2015, Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/4.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC. |
spellingShingle | Medical Microbiology Naz, Sehar Afshan Jabeen, Nusrat Sohail, Muhammad Rasool, Sheikh Ajaz Biophysicochemical characterization of Pyocin SA189 produced by Pseudomonas aeruginosa SA189 |
title | Biophysicochemical characterization of Pyocin SA189 produced by
Pseudomonas aeruginosa SA189 |
title_full | Biophysicochemical characterization of Pyocin SA189 produced by
Pseudomonas aeruginosa SA189 |
title_fullStr | Biophysicochemical characterization of Pyocin SA189 produced by
Pseudomonas aeruginosa SA189 |
title_full_unstemmed | Biophysicochemical characterization of Pyocin SA189 produced by
Pseudomonas aeruginosa SA189 |
title_short | Biophysicochemical characterization of Pyocin SA189 produced by
Pseudomonas aeruginosa SA189 |
title_sort | biophysicochemical characterization of pyocin sa189 produced by
pseudomonas aeruginosa sa189 |
topic | Medical Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4704615/ https://www.ncbi.nlm.nih.gov/pubmed/26691474 http://dx.doi.org/10.1590/S1517-838246420140737 |
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