Production of Brazilian human norovirus VLPs and comparison of purification methods

Noroviruses (NVs) are responsible for most cases of human nonbacterial gastroenteritis worldwide. Some parameters for the purification of NV virus-like particles (VLPs) such as ease of production and yield were studied for future development of vaccines and diagnostic tools. In this study, VLPs were...

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Detalles Bibliográficos
Autores principales: Lamounier, Thais Alves da Costa, de Oliveira, Layssa Miranda, de Camargo, Brenda Rabello, Rodrigues, Kelly Barreto, Noronha, Eliane Ferreira, Ribeiro, Bergmann Morais, Nagata, Tatsuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2015
Materias:
Genetics and Molecular Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4704647/
https://www.ncbi.nlm.nih.gov/pubmed/26691489
http://dx.doi.org/10.1590/S1517-838246420140925
Descripción
Sumario:Noroviruses (NVs) are responsible for most cases of human nonbacterial gastroenteritis worldwide. Some parameters for the purification of NV virus-like particles (VLPs) such as ease of production and yield were studied for future development of vaccines and diagnostic tools. In this study, VLPs were produced by the expression of the VP1 and VP2 gene cassette of the Brazilian NV isolate, and two purification methods were compared: cesium chloride (CsCl) gradient centrifugation and ion-exchange chromatography (IEC). IEC produced more and purer VLPs of NV compared to CsCl gradient centrifugation.

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