Production of Brazilian human norovirus VLPs and comparison of purification methods

Noroviruses (NVs) are responsible for most cases of human nonbacterial gastroenteritis worldwide. Some parameters for the purification of NV virus-like particles (VLPs) such as ease of production and yield were studied for future development of vaccines and diagnostic tools. In this study, VLPs were...

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Autores principales: Lamounier, Thais Alves da Costa, de Oliveira, Layssa Miranda, de Camargo, Brenda Rabello, Rodrigues, Kelly Barreto, Noronha, Eliane Ferreira, Ribeiro, Bergmann Morais, Nagata, Tatsuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2015
Materias:
Genetics and Molecular Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4704647/
https://www.ncbi.nlm.nih.gov/pubmed/26691489
http://dx.doi.org/10.1590/S1517-838246420140925
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author Lamounier, Thais Alves da Costa
de Oliveira, Layssa Miranda
de Camargo, Brenda Rabello
Rodrigues, Kelly Barreto
Noronha, Eliane Ferreira
Ribeiro, Bergmann Morais
Nagata, Tatsuya
author_facet Lamounier, Thais Alves da Costa
de Oliveira, Layssa Miranda
de Camargo, Brenda Rabello
Rodrigues, Kelly Barreto
Noronha, Eliane Ferreira
Ribeiro, Bergmann Morais
Nagata, Tatsuya
author_sort Lamounier, Thais Alves da Costa
collection PubMed
description Noroviruses (NVs) are responsible for most cases of human nonbacterial gastroenteritis worldwide. Some parameters for the purification of NV virus-like particles (VLPs) such as ease of production and yield were studied for future development of vaccines and diagnostic tools. In this study, VLPs were produced by the expression of the VP1 and VP2 gene cassette of the Brazilian NV isolate, and two purification methods were compared: cesium chloride (CsCl) gradient centrifugation and ion-exchange chromatography (IEC). IEC produced more and purer VLPs of NV compared to CsCl gradient centrifugation.
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spelling pubmed-47046472016-01-14 Production of Brazilian human norovirus VLPs and comparison of purification methods Lamounier, Thais Alves da Costa de Oliveira, Layssa Miranda de Camargo, Brenda Rabello Rodrigues, Kelly Barreto Noronha, Eliane Ferreira Ribeiro, Bergmann Morais Nagata, Tatsuya Braz J Microbiol Genetics and Molecular Microbiology Noroviruses (NVs) are responsible for most cases of human nonbacterial gastroenteritis worldwide. Some parameters for the purification of NV virus-like particles (VLPs) such as ease of production and yield were studied for future development of vaccines and diagnostic tools. In this study, VLPs were produced by the expression of the VP1 and VP2 gene cassette of the Brazilian NV isolate, and two purification methods were compared: cesium chloride (CsCl) gradient centrifugation and ion-exchange chromatography (IEC). IEC produced more and purer VLPs of NV compared to CsCl gradient centrifugation. Sociedade Brasileira de Microbiologia 2015-12-01 /pmc/articles/PMC4704647/ /pubmed/26691489 http://dx.doi.org/10.1590/S1517-838246420140925 Text en Copyright © 2015, Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/4.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC-BY.
spellingShingle Genetics and Molecular Microbiology
Lamounier, Thais Alves da Costa
de Oliveira, Layssa Miranda
de Camargo, Brenda Rabello
Rodrigues, Kelly Barreto
Noronha, Eliane Ferreira
Ribeiro, Bergmann Morais
Nagata, Tatsuya
Production of Brazilian human norovirus VLPs and comparison of purification methods
title Production of Brazilian human norovirus VLPs and comparison of purification methods
title_full Production of Brazilian human norovirus VLPs and comparison of purification methods
title_fullStr Production of Brazilian human norovirus VLPs and comparison of purification methods
title_full_unstemmed Production of Brazilian human norovirus VLPs and comparison of purification methods
title_short Production of Brazilian human norovirus VLPs and comparison of purification methods
title_sort production of brazilian human norovirus vlps and comparison of purification methods
topic Genetics and Molecular Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4704647/
https://www.ncbi.nlm.nih.gov/pubmed/26691489
http://dx.doi.org/10.1590/S1517-838246420140925
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