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High occurrence of Fusobacterium nucleatum and Clostridium difficile in the intestinal microbiota of colorectal carcinoma patients
Colorectal carcinoma is considered the fourth leading cause of cancer deaths worldwide. Several microorganisms have been associated with carcinogenesis, including Enterococcus spp., Helicobacter pylori, enterotoxigenic Bacteroides fragilis, pathogenic E. coli strains and oral Fusobacterium. Here we...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Microbiologia
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4704648/ https://www.ncbi.nlm.nih.gov/pubmed/26691472 http://dx.doi.org/10.1590/S1517-838246420140665 |
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author | Fukugaiti, Márcia H. Ignacio, Aline Fernandes, Miriam R. Ribeiro, Ulysses Nakano, Viviane Avila-Campos, Mario J. |
author_facet | Fukugaiti, Márcia H. Ignacio, Aline Fernandes, Miriam R. Ribeiro, Ulysses Nakano, Viviane Avila-Campos, Mario J. |
author_sort | Fukugaiti, Márcia H. |
collection | PubMed |
description | Colorectal carcinoma is considered the fourth leading cause of cancer deaths worldwide. Several microorganisms have been associated with carcinogenesis, including Enterococcus spp., Helicobacter pylori, enterotoxigenic Bacteroides fragilis, pathogenic E. coli strains and oral Fusobacterium. Here we qualitatively and quantitatively evaluated the presence of oral and intestinal microorganisms in the fecal microbiota of colorectal cancer patients and healthy controls. Seventeen patients (between 49 and 70 years-old) visiting the Cancer Institute of the Sao Paulo State were selected, 7 of whom were diagnosed with colorectal carcinoma. Bacterial detection was performed by qRT-PCR. Although all of the tested bacteria were detected in the majority of the fecal samples, quantitative differences between the Cancer Group and healthy controls were detected only for F. nucleatum and C. difficile. The three tested oral microorganisms were frequently observed, suggesting a need for furthers studies into a potential role for these bacteria during colorectal carcinoma pathogenesis. Despite the small number of patients included in this study, we were able to detect significantly more F. nucleatum and C. difficile in the Cancer Group patients compared to healthy controls, suggesting a possible role of these bacteria in colon carcinogenesis. This finding should be considered when screening for colorectal cancer. |
format | Online Article Text |
id | pubmed-4704648 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Sociedade Brasileira de Microbiologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-47046482016-01-14 High occurrence of Fusobacterium nucleatum and Clostridium difficile in the intestinal microbiota of colorectal carcinoma patients Fukugaiti, Márcia H. Ignacio, Aline Fernandes, Miriam R. Ribeiro, Ulysses Nakano, Viviane Avila-Campos, Mario J. Braz J Microbiol Medical Microbiology Colorectal carcinoma is considered the fourth leading cause of cancer deaths worldwide. Several microorganisms have been associated with carcinogenesis, including Enterococcus spp., Helicobacter pylori, enterotoxigenic Bacteroides fragilis, pathogenic E. coli strains and oral Fusobacterium. Here we qualitatively and quantitatively evaluated the presence of oral and intestinal microorganisms in the fecal microbiota of colorectal cancer patients and healthy controls. Seventeen patients (between 49 and 70 years-old) visiting the Cancer Institute of the Sao Paulo State were selected, 7 of whom were diagnosed with colorectal carcinoma. Bacterial detection was performed by qRT-PCR. Although all of the tested bacteria were detected in the majority of the fecal samples, quantitative differences between the Cancer Group and healthy controls were detected only for F. nucleatum and C. difficile. The three tested oral microorganisms were frequently observed, suggesting a need for furthers studies into a potential role for these bacteria during colorectal carcinoma pathogenesis. Despite the small number of patients included in this study, we were able to detect significantly more F. nucleatum and C. difficile in the Cancer Group patients compared to healthy controls, suggesting a possible role of these bacteria in colon carcinogenesis. This finding should be considered when screening for colorectal cancer. Sociedade Brasileira de Microbiologia 2015-12-01 /pmc/articles/PMC4704648/ /pubmed/26691472 http://dx.doi.org/10.1590/S1517-838246420140665 Text en Copyright © 2015, Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/4.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC-BY. |
spellingShingle | Medical Microbiology Fukugaiti, Márcia H. Ignacio, Aline Fernandes, Miriam R. Ribeiro, Ulysses Nakano, Viviane Avila-Campos, Mario J. High occurrence of Fusobacterium nucleatum and Clostridium difficile in the intestinal microbiota of colorectal carcinoma patients |
title | High occurrence of Fusobacterium nucleatum and
Clostridium difficile in the intestinal microbiota of colorectal
carcinoma patients |
title_full | High occurrence of Fusobacterium nucleatum and
Clostridium difficile in the intestinal microbiota of colorectal
carcinoma patients |
title_fullStr | High occurrence of Fusobacterium nucleatum and
Clostridium difficile in the intestinal microbiota of colorectal
carcinoma patients |
title_full_unstemmed | High occurrence of Fusobacterium nucleatum and
Clostridium difficile in the intestinal microbiota of colorectal
carcinoma patients |
title_short | High occurrence of Fusobacterium nucleatum and
Clostridium difficile in the intestinal microbiota of colorectal
carcinoma patients |
title_sort | high occurrence of fusobacterium nucleatum and
clostridium difficile in the intestinal microbiota of colorectal
carcinoma patients |
topic | Medical Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4704648/ https://www.ncbi.nlm.nih.gov/pubmed/26691472 http://dx.doi.org/10.1590/S1517-838246420140665 |
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