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Optimization of immunostaining on flat-mounted human corneas

PURPOSE: In the literature, immunohistochemistry on cross sections is the main technique used to study protein expression in corneal endothelial cells (ECs), even though this method allows visualization of few ECs, without clear subcellular localization, and is subject to the staining artifacts freq...

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Detalles Bibliográficos
Autores principales: Forest, Fabien, Thuret, Gilles, Gain, Philippe, Dumollard, Jean-Marc, Peoc’h, Michel, Perrache, Chantal, He, Zhiguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Vision 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4704774/
https://www.ncbi.nlm.nih.gov/pubmed/26788027