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A Rapid Protocol of Crude RNA/DNA Extraction for RT-qPCR Detection and Quantification of 'Candidatus Phytoplasma prunorum'

Many efforts have been made to develop a rapid and sensitive method for phytoplasma and virus detection. Taking our cue from previous works, different rapid sample preparation methods have been tested and applied to Candidatus Phytoplasma prunorum (‘Ca. P. prunorum’) detection by RT-qPCR. A duplex R...

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Detalles Bibliográficos
Autores principales: Minguzzi, Stefano, Terlizzi, Federica, Lanzoni, Chiara, Poggi Pollini, Carlo, Ratti, Claudio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4704776/
https://www.ncbi.nlm.nih.gov/pubmed/26742106
http://dx.doi.org/10.1371/journal.pone.0146515