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Comparative studies for serodiagnosis of haemorrhagic septicaemia in cattle sera
Haemorrhagic septicaemia caused by Pasteurella multocida is a major epizootic disease in cattle and buffaloes in developing countries with high morbidity and mortality rate. In the present study, a total of 88 P. multocida isolates were isolated from 256 nasopharyngeal swabs and lung tissues samples...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4705243/ https://www.ncbi.nlm.nih.gov/pubmed/26858538 http://dx.doi.org/10.1016/j.sjbs.2015.06.011 |
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author | El-Jakee, Jakeen K. Ali, Samah Said El-Shafii, Soumaya Ahmed Hessain, Ashgan M. Al-Arfaj, Abdullah A. Mohamed, Moussa I. |
author_facet | El-Jakee, Jakeen K. Ali, Samah Said El-Shafii, Soumaya Ahmed Hessain, Ashgan M. Al-Arfaj, Abdullah A. Mohamed, Moussa I. |
author_sort | El-Jakee, Jakeen K. |
collection | PubMed |
description | Haemorrhagic septicaemia caused by Pasteurella multocida is a major epizootic disease in cattle and buffaloes in developing countries with high morbidity and mortality rate. In the present study, a total of 88 P. multocida isolates were isolated from 256 nasopharyngeal swabs and lung tissues samples (34.4%) during the period from January, 2013 to March, 2014 from different governorates located in Egypt. Dead calves showed the highest percentage of P. multocida isolation followed by the emergency slaughtered calves, diseased calves then apparently healthy ones. These isolates were confirmed as P. multocida microscopically, biochemically by traditional tests and by API 20E commercial kit then by PCR. The percentages of positive serum samples using somatic antigen and micro-agglutination test at 1/1280 diluted serum were 10%, 54.49% and 0% in apparently healthy, diseased and emergency slaughtered samples, respectively whereas, the percentages using capsular antigen and indirect haemagglutination test were 40%, 60.89% and 60% in apparently healthy, diseased and emergency slaughtered samples, respectively. The ELISA showed the highest sensitivity for diagnosing P. multocida in apparently healthy, diseased and emergency slaughtered animals with percentages of 42%; 92.9% and 80%, respectively. The obtained results revealed that the ELISA using capsular antigen of P. multocida is a more sensitive and specific serological test for diagnosis of haemorrhagic septicaemia. |
format | Online Article Text |
id | pubmed-4705243 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-47052432016-02-08 Comparative studies for serodiagnosis of haemorrhagic septicaemia in cattle sera El-Jakee, Jakeen K. Ali, Samah Said El-Shafii, Soumaya Ahmed Hessain, Ashgan M. Al-Arfaj, Abdullah A. Mohamed, Moussa I. Saudi J Biol Sci Original Article Haemorrhagic septicaemia caused by Pasteurella multocida is a major epizootic disease in cattle and buffaloes in developing countries with high morbidity and mortality rate. In the present study, a total of 88 P. multocida isolates were isolated from 256 nasopharyngeal swabs and lung tissues samples (34.4%) during the period from January, 2013 to March, 2014 from different governorates located in Egypt. Dead calves showed the highest percentage of P. multocida isolation followed by the emergency slaughtered calves, diseased calves then apparently healthy ones. These isolates were confirmed as P. multocida microscopically, biochemically by traditional tests and by API 20E commercial kit then by PCR. The percentages of positive serum samples using somatic antigen and micro-agglutination test at 1/1280 diluted serum were 10%, 54.49% and 0% in apparently healthy, diseased and emergency slaughtered samples, respectively whereas, the percentages using capsular antigen and indirect haemagglutination test were 40%, 60.89% and 60% in apparently healthy, diseased and emergency slaughtered samples, respectively. The ELISA showed the highest sensitivity for diagnosing P. multocida in apparently healthy, diseased and emergency slaughtered animals with percentages of 42%; 92.9% and 80%, respectively. The obtained results revealed that the ELISA using capsular antigen of P. multocida is a more sensitive and specific serological test for diagnosis of haemorrhagic septicaemia. Elsevier 2016-01 2015-06-16 /pmc/articles/PMC4705243/ /pubmed/26858538 http://dx.doi.org/10.1016/j.sjbs.2015.06.011 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article El-Jakee, Jakeen K. Ali, Samah Said El-Shafii, Soumaya Ahmed Hessain, Ashgan M. Al-Arfaj, Abdullah A. Mohamed, Moussa I. Comparative studies for serodiagnosis of haemorrhagic septicaemia in cattle sera |
title | Comparative studies for serodiagnosis of haemorrhagic septicaemia in cattle sera |
title_full | Comparative studies for serodiagnosis of haemorrhagic septicaemia in cattle sera |
title_fullStr | Comparative studies for serodiagnosis of haemorrhagic septicaemia in cattle sera |
title_full_unstemmed | Comparative studies for serodiagnosis of haemorrhagic septicaemia in cattle sera |
title_short | Comparative studies for serodiagnosis of haemorrhagic septicaemia in cattle sera |
title_sort | comparative studies for serodiagnosis of haemorrhagic septicaemia in cattle sera |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4705243/ https://www.ncbi.nlm.nih.gov/pubmed/26858538 http://dx.doi.org/10.1016/j.sjbs.2015.06.011 |
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