Cargando…

MicroRNAs: a novel promising therapeutic target for cerebral ischemia/reperfusion injury?

To determine the molecular mechanism of cerebral ischemia/reperfusion injury, we examined the microRNA (miRNA) expression profile in rat cortex after focal cerebral ischemia/reperfusion injury using miRNA microarrays and bioinformatic tools to systematically analyze Gene Ontology (GO) function class...

Descripción completa

Detalles Bibliográficos
Autores principales: Min, Xiao-li, Wang, Ting-yong, Cao, Yi, Liu, Jia, Li, Jin-tao, Wang, Ting-hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4705791/
https://www.ncbi.nlm.nih.gov/pubmed/26807114
http://dx.doi.org/10.4103/1673-5374.170302
_version_ 1782409083337310208
author Min, Xiao-li
Wang, Ting-yong
Cao, Yi
Liu, Jia
Li, Jin-tao
Wang, Ting-hua
author_facet Min, Xiao-li
Wang, Ting-yong
Cao, Yi
Liu, Jia
Li, Jin-tao
Wang, Ting-hua
author_sort Min, Xiao-li
collection PubMed
description To determine the molecular mechanism of cerebral ischemia/reperfusion injury, we examined the microRNA (miRNA) expression profile in rat cortex after focal cerebral ischemia/reperfusion injury using miRNA microarrays and bioinformatic tools to systematically analyze Gene Ontology (GO) function classifications, as well as the signaling pathways of genes targeted by these differentially expressed miRNAs. Our results show significantly changed miRNA expression profiles in the reperfusion period after focal cerebral ischemia, with a total of 15 miRNAs up-regulated and 44 miRNAs down-regulated. Target genes of these differentially expressed miRNAs were mainly involved in metabolic and cellular processes, which were identified as hub nodes of a miRNA-GO-network. The most correlated pathways included D-glutamine and D-glutamate metabolism, the renin-angiotensin system, peroxisomes, the PPAR signaling pathway, SNARE interactions in vesicular transport, and the calcium signaling pathway. Our study suggests that miRNAs play an important role in the pathological process of cerebral ischemia/reperfusion injury. Understanding miRNA expression and function may shed light on the molecular mechanism of cerebral ischemia/reperfusion injury.
format Online
Article
Text
id pubmed-4705791
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Medknow Publications & Media Pvt Ltd
record_format MEDLINE/PubMed
spelling pubmed-47057912016-01-22 MicroRNAs: a novel promising therapeutic target for cerebral ischemia/reperfusion injury? Min, Xiao-li Wang, Ting-yong Cao, Yi Liu, Jia Li, Jin-tao Wang, Ting-hua Neural Regen Res Research Article To determine the molecular mechanism of cerebral ischemia/reperfusion injury, we examined the microRNA (miRNA) expression profile in rat cortex after focal cerebral ischemia/reperfusion injury using miRNA microarrays and bioinformatic tools to systematically analyze Gene Ontology (GO) function classifications, as well as the signaling pathways of genes targeted by these differentially expressed miRNAs. Our results show significantly changed miRNA expression profiles in the reperfusion period after focal cerebral ischemia, with a total of 15 miRNAs up-regulated and 44 miRNAs down-regulated. Target genes of these differentially expressed miRNAs were mainly involved in metabolic and cellular processes, which were identified as hub nodes of a miRNA-GO-network. The most correlated pathways included D-glutamine and D-glutamate metabolism, the renin-angiotensin system, peroxisomes, the PPAR signaling pathway, SNARE interactions in vesicular transport, and the calcium signaling pathway. Our study suggests that miRNAs play an important role in the pathological process of cerebral ischemia/reperfusion injury. Understanding miRNA expression and function may shed light on the molecular mechanism of cerebral ischemia/reperfusion injury. Medknow Publications & Media Pvt Ltd 2015-11 /pmc/articles/PMC4705791/ /pubmed/26807114 http://dx.doi.org/10.4103/1673-5374.170302 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Research Article
Min, Xiao-li
Wang, Ting-yong
Cao, Yi
Liu, Jia
Li, Jin-tao
Wang, Ting-hua
MicroRNAs: a novel promising therapeutic target for cerebral ischemia/reperfusion injury?
title MicroRNAs: a novel promising therapeutic target for cerebral ischemia/reperfusion injury?
title_full MicroRNAs: a novel promising therapeutic target for cerebral ischemia/reperfusion injury?
title_fullStr MicroRNAs: a novel promising therapeutic target for cerebral ischemia/reperfusion injury?
title_full_unstemmed MicroRNAs: a novel promising therapeutic target for cerebral ischemia/reperfusion injury?
title_short MicroRNAs: a novel promising therapeutic target for cerebral ischemia/reperfusion injury?
title_sort micrornas: a novel promising therapeutic target for cerebral ischemia/reperfusion injury?
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4705791/
https://www.ncbi.nlm.nih.gov/pubmed/26807114
http://dx.doi.org/10.4103/1673-5374.170302
work_keys_str_mv AT minxiaoli micrornasanovelpromisingtherapeutictargetforcerebralischemiareperfusioninjury
AT wangtingyong micrornasanovelpromisingtherapeutictargetforcerebralischemiareperfusioninjury
AT caoyi micrornasanovelpromisingtherapeutictargetforcerebralischemiareperfusioninjury
AT liujia micrornasanovelpromisingtherapeutictargetforcerebralischemiareperfusioninjury
AT lijintao micrornasanovelpromisingtherapeutictargetforcerebralischemiareperfusioninjury
AT wangtinghua micrornasanovelpromisingtherapeutictargetforcerebralischemiareperfusioninjury