Cargando…
Arabidopsis ERF1 Mediates Cross-Talk between Ethylene and Auxin Biosynthesis during Primary Root Elongation by Regulating ASA1 Expression
The gaseous phytohormone ethylene participates in the regulation of root growth and development in Arabidopsis. It is known that root growth inhibition by ethylene involves auxin, which is partially mediated by the action of the WEAK ETHYLENE INSENSITIVE2/ANTHRANILATE SYNTHASE α1 (WEI2/ASA1), encodi...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4706318/ https://www.ncbi.nlm.nih.gov/pubmed/26745809 http://dx.doi.org/10.1371/journal.pgen.1005760 |
_version_ | 1782409146229850112 |
---|---|
author | Mao, Jie-Li Miao, Zi-Qing Wang, Zhen Yu, Lin-Hui Cai, Xiao-Teng Xiang, Cheng-Bin |
author_facet | Mao, Jie-Li Miao, Zi-Qing Wang, Zhen Yu, Lin-Hui Cai, Xiao-Teng Xiang, Cheng-Bin |
author_sort | Mao, Jie-Li |
collection | PubMed |
description | The gaseous phytohormone ethylene participates in the regulation of root growth and development in Arabidopsis. It is known that root growth inhibition by ethylene involves auxin, which is partially mediated by the action of the WEAK ETHYLENE INSENSITIVE2/ANTHRANILATE SYNTHASE α1 (WEI2/ASA1), encoding a rate-limiting enzyme in tryptophan (Trp) biosynthesis, from which auxin is derived. However, the molecular mechanism by which ethylene decreases root growth via ASA1 is not understood. Here we report that the ethylene-responsive AP2 transcription factor, ETHYLENE RESPONSE FACTOR1 (ERF1), plays an important role in primary root elongation of Arabidopsis. Using loss- and gain-of-function transgenic lines as well as biochemical analysis, we demonstrate that ERF1 can directly up-regulate ASA1 by binding to its promoter, leading to auxin accumulation and ethylene-induced inhibition of root growth. This discloses one mechanism linking ethylene signaling and auxin biosynthesis in Arabidopsis roots. |
format | Online Article Text |
id | pubmed-4706318 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-47063182016-01-15 Arabidopsis ERF1 Mediates Cross-Talk between Ethylene and Auxin Biosynthesis during Primary Root Elongation by Regulating ASA1 Expression Mao, Jie-Li Miao, Zi-Qing Wang, Zhen Yu, Lin-Hui Cai, Xiao-Teng Xiang, Cheng-Bin PLoS Genet Research Article The gaseous phytohormone ethylene participates in the regulation of root growth and development in Arabidopsis. It is known that root growth inhibition by ethylene involves auxin, which is partially mediated by the action of the WEAK ETHYLENE INSENSITIVE2/ANTHRANILATE SYNTHASE α1 (WEI2/ASA1), encoding a rate-limiting enzyme in tryptophan (Trp) biosynthesis, from which auxin is derived. However, the molecular mechanism by which ethylene decreases root growth via ASA1 is not understood. Here we report that the ethylene-responsive AP2 transcription factor, ETHYLENE RESPONSE FACTOR1 (ERF1), plays an important role in primary root elongation of Arabidopsis. Using loss- and gain-of-function transgenic lines as well as biochemical analysis, we demonstrate that ERF1 can directly up-regulate ASA1 by binding to its promoter, leading to auxin accumulation and ethylene-induced inhibition of root growth. This discloses one mechanism linking ethylene signaling and auxin biosynthesis in Arabidopsis roots. Public Library of Science 2016-01-08 /pmc/articles/PMC4706318/ /pubmed/26745809 http://dx.doi.org/10.1371/journal.pgen.1005760 Text en © 2016 Mao et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited |
spellingShingle | Research Article Mao, Jie-Li Miao, Zi-Qing Wang, Zhen Yu, Lin-Hui Cai, Xiao-Teng Xiang, Cheng-Bin Arabidopsis ERF1 Mediates Cross-Talk between Ethylene and Auxin Biosynthesis during Primary Root Elongation by Regulating ASA1 Expression |
title | Arabidopsis ERF1 Mediates Cross-Talk between Ethylene and Auxin Biosynthesis during Primary Root Elongation by Regulating ASA1 Expression |
title_full | Arabidopsis ERF1 Mediates Cross-Talk between Ethylene and Auxin Biosynthesis during Primary Root Elongation by Regulating ASA1 Expression |
title_fullStr | Arabidopsis ERF1 Mediates Cross-Talk between Ethylene and Auxin Biosynthesis during Primary Root Elongation by Regulating ASA1 Expression |
title_full_unstemmed | Arabidopsis ERF1 Mediates Cross-Talk between Ethylene and Auxin Biosynthesis during Primary Root Elongation by Regulating ASA1 Expression |
title_short | Arabidopsis ERF1 Mediates Cross-Talk between Ethylene and Auxin Biosynthesis during Primary Root Elongation by Regulating ASA1 Expression |
title_sort | arabidopsis erf1 mediates cross-talk between ethylene and auxin biosynthesis during primary root elongation by regulating asa1 expression |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4706318/ https://www.ncbi.nlm.nih.gov/pubmed/26745809 http://dx.doi.org/10.1371/journal.pgen.1005760 |
work_keys_str_mv | AT maojieli arabidopsiserf1mediatescrosstalkbetweenethyleneandauxinbiosynthesisduringprimaryrootelongationbyregulatingasa1expression AT miaoziqing arabidopsiserf1mediatescrosstalkbetweenethyleneandauxinbiosynthesisduringprimaryrootelongationbyregulatingasa1expression AT wangzhen arabidopsiserf1mediatescrosstalkbetweenethyleneandauxinbiosynthesisduringprimaryrootelongationbyregulatingasa1expression AT yulinhui arabidopsiserf1mediatescrosstalkbetweenethyleneandauxinbiosynthesisduringprimaryrootelongationbyregulatingasa1expression AT caixiaoteng arabidopsiserf1mediatescrosstalkbetweenethyleneandauxinbiosynthesisduringprimaryrootelongationbyregulatingasa1expression AT xiangchengbin arabidopsiserf1mediatescrosstalkbetweenethyleneandauxinbiosynthesisduringprimaryrootelongationbyregulatingasa1expression |