Vinculin expression in MC3T3-E1 cells in response to mechanical stimulus

Loading frequency is known to influence the expression of the focal adhesions of the adherent cells. A small cyclical tensile force was transmitted to mouse pre-osteoblast MC3T3-E1 cells through PDMS substrates of varying stiffness. Changes in cell behavior with respect to proliferation and characte...

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Detalles Bibliográficos
Autores principales: Cora-Cruz, J.J., Diffoot-Carlo, N., Sundaram, P.A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Data Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4706558/
https://www.ncbi.nlm.nih.gov/pubmed/26858974
http://dx.doi.org/10.1016/j.dib.2015.11.052
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author Cora-Cruz, J.J.
Diffoot-Carlo, N.
Sundaram, P.A.
author_facet Cora-Cruz, J.J.
Diffoot-Carlo, N.
Sundaram, P.A.
author_sort Cora-Cruz, J.J.
collection PubMed
description Loading frequency is known to influence the expression of the focal adhesions of the adherent cells. A small cyclical tensile force was transmitted to mouse pre-osteoblast MC3T3-E1 cells through PDMS substrates of varying stiffness. Changes in cell behavior with respect to proliferation and characteristics of focal adhesions were quantified through immunofluorescence labeling of vinculin. Amount of inactive vinculin was higher on substrates subjected to cyclic stimulation when compared with the results of the static substrates, whereas the number and area of focal adhesion points underwent a reduction. Inactive vinculin appears as a cloud in the cytoplasm in the vicinity of the nucleus.
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spelling pubmed-47065582016-02-08 Vinculin expression in MC3T3-E1 cells in response to mechanical stimulus Cora-Cruz, J.J. Diffoot-Carlo, N. Sundaram, P.A. Data Brief Data Article Loading frequency is known to influence the expression of the focal adhesions of the adherent cells. A small cyclical tensile force was transmitted to mouse pre-osteoblast MC3T3-E1 cells through PDMS substrates of varying stiffness. Changes in cell behavior with respect to proliferation and characteristics of focal adhesions were quantified through immunofluorescence labeling of vinculin. Amount of inactive vinculin was higher on substrates subjected to cyclic stimulation when compared with the results of the static substrates, whereas the number and area of focal adhesion points underwent a reduction. Inactive vinculin appears as a cloud in the cytoplasm in the vicinity of the nucleus. Elsevier 2015-12-05 /pmc/articles/PMC4706558/ /pubmed/26858974 http://dx.doi.org/10.1016/j.dib.2015.11.052 Text en © 2015 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Cora-Cruz, J.J.
Diffoot-Carlo, N.
Sundaram, P.A.
Vinculin expression in MC3T3-E1 cells in response to mechanical stimulus
title Vinculin expression in MC3T3-E1 cells in response to mechanical stimulus
title_full Vinculin expression in MC3T3-E1 cells in response to mechanical stimulus
title_fullStr Vinculin expression in MC3T3-E1 cells in response to mechanical stimulus
title_full_unstemmed Vinculin expression in MC3T3-E1 cells in response to mechanical stimulus
title_short Vinculin expression in MC3T3-E1 cells in response to mechanical stimulus
title_sort vinculin expression in mc3t3-e1 cells in response to mechanical stimulus
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4706558/
https://www.ncbi.nlm.nih.gov/pubmed/26858974
http://dx.doi.org/10.1016/j.dib.2015.11.052
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