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REPLACR-mutagenesis, a one-step method for site-directed mutagenesis by recombineering

Mutagenesis is an important tool to study gene regulation, model disease-causing mutations and for functional characterisation of proteins. Most of the current methods for mutagenesis involve multiple step procedures. One of the most accurate methods for genetically altering DNA is recombineering, w...

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Detalles Bibliográficos
Autores principales:	Trehan, Ashutosh, Kiełbus, Michał, Czapinski, Jakub, Stepulak, Andrzej, Huhtaniemi, Ilpo, Rivero-Müller, Adolfo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4707547/ https://www.ncbi.nlm.nih.gov/pubmed/26750263 http://dx.doi.org/10.1038/srep19121

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