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Leishmania amazonensis DNA in wild females of Lutzomyia cruzi (Diptera: Psychodidae) in the state of Mato Grosso do Sul, Brazil

Studies on natural infection by Leishmania spp of sandflies collected in endemic and nonendemic areas can provide important information on the distribution and intensity of the transmission of these parasites. This study sought to investigate the natural infection by Leishmaniain wild female sandfli...

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Autores principales: de Oliveira, Everton Falcão, Casaril, Aline Etelvina, Mateus, Nathália Lopes Fontoura, Murat, Paula Guerra, Fernandes, Wagner Souza, Oshiro, Elisa Teruya, de Oliveira, Alessandra Gutierrez, Galati, Eunice Aparecida Bianchi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz, Ministério da Saúde 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4708026/
https://www.ncbi.nlm.nih.gov/pubmed/26602870
http://dx.doi.org/10.1590/0074-02760150317
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author de Oliveira, Everton Falcão
Casaril, Aline Etelvina
Mateus, Nathália Lopes Fontoura
Murat, Paula Guerra
Fernandes, Wagner Souza
Oshiro, Elisa Teruya
de Oliveira, Alessandra Gutierrez
Galati, Eunice Aparecida Bianchi
author_facet de Oliveira, Everton Falcão
Casaril, Aline Etelvina
Mateus, Nathália Lopes Fontoura
Murat, Paula Guerra
Fernandes, Wagner Souza
Oshiro, Elisa Teruya
de Oliveira, Alessandra Gutierrez
Galati, Eunice Aparecida Bianchi
author_sort de Oliveira, Everton Falcão
collection PubMed
description Studies on natural infection by Leishmania spp of sandflies collected in endemic and nonendemic areas can provide important information on the distribution and intensity of the transmission of these parasites. This study sought to investigate the natural infection by Leishmaniain wild female sandflies. The specimens were caught in the city of Corumbá, state of Mato Grosso do Sul (Brazil) between October 2012-March 2014, and dissected to investigate flagellates and/or submitted to molecular analysis to detect Leishmania DNA. A total of 1,164 females (77.56% of which were Lutzomyia cruzi) representing 11 species were investigated using molecular analysis; 126 specimens of Lu. cruziwere dissected and also submitted to molecular analysis. The infection rate based on the presence of Leishmania DNA considering all the sandfly species analysed was 0.69%; only Leishmania (Leishmania) amazonensis was identified in Lu. cruzi by the molecular analysis. The dissections were negative for flagellates. This is the first record of the presence of L. (L.) amazonensis DNA in Lu. cruzi, and the first record of this parasite in this area. These findings point to the need for further investigation into the possible role of this sandfly as vector of this parasite.
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spelling pubmed-47080262016-01-26 Leishmania amazonensis DNA in wild females of Lutzomyia cruzi (Diptera: Psychodidae) in the state of Mato Grosso do Sul, Brazil de Oliveira, Everton Falcão Casaril, Aline Etelvina Mateus, Nathália Lopes Fontoura Murat, Paula Guerra Fernandes, Wagner Souza Oshiro, Elisa Teruya de Oliveira, Alessandra Gutierrez Galati, Eunice Aparecida Bianchi Mem Inst Oswaldo Cruz Articles Studies on natural infection by Leishmania spp of sandflies collected in endemic and nonendemic areas can provide important information on the distribution and intensity of the transmission of these parasites. This study sought to investigate the natural infection by Leishmaniain wild female sandflies. The specimens were caught in the city of Corumbá, state of Mato Grosso do Sul (Brazil) between October 2012-March 2014, and dissected to investigate flagellates and/or submitted to molecular analysis to detect Leishmania DNA. A total of 1,164 females (77.56% of which were Lutzomyia cruzi) representing 11 species were investigated using molecular analysis; 126 specimens of Lu. cruziwere dissected and also submitted to molecular analysis. The infection rate based on the presence of Leishmania DNA considering all the sandfly species analysed was 0.69%; only Leishmania (Leishmania) amazonensis was identified in Lu. cruzi by the molecular analysis. The dissections were negative for flagellates. This is the first record of the presence of L. (L.) amazonensis DNA in Lu. cruzi, and the first record of this parasite in this area. These findings point to the need for further investigation into the possible role of this sandfly as vector of this parasite. Instituto Oswaldo Cruz, Ministério da Saúde 2015-12 /pmc/articles/PMC4708026/ /pubmed/26602870 http://dx.doi.org/10.1590/0074-02760150317 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
de Oliveira, Everton Falcão
Casaril, Aline Etelvina
Mateus, Nathália Lopes Fontoura
Murat, Paula Guerra
Fernandes, Wagner Souza
Oshiro, Elisa Teruya
de Oliveira, Alessandra Gutierrez
Galati, Eunice Aparecida Bianchi
Leishmania amazonensis DNA in wild females of Lutzomyia cruzi (Diptera: Psychodidae) in the state of Mato Grosso do Sul, Brazil
title Leishmania amazonensis DNA in wild females of Lutzomyia cruzi (Diptera: Psychodidae) in the state of Mato Grosso do Sul, Brazil
title_full Leishmania amazonensis DNA in wild females of Lutzomyia cruzi (Diptera: Psychodidae) in the state of Mato Grosso do Sul, Brazil
title_fullStr Leishmania amazonensis DNA in wild females of Lutzomyia cruzi (Diptera: Psychodidae) in the state of Mato Grosso do Sul, Brazil
title_full_unstemmed Leishmania amazonensis DNA in wild females of Lutzomyia cruzi (Diptera: Psychodidae) in the state of Mato Grosso do Sul, Brazil
title_short Leishmania amazonensis DNA in wild females of Lutzomyia cruzi (Diptera: Psychodidae) in the state of Mato Grosso do Sul, Brazil
title_sort leishmania amazonensis dna in wild females of lutzomyia cruzi (diptera: psychodidae) in the state of mato grosso do sul, brazil
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4708026/
https://www.ncbi.nlm.nih.gov/pubmed/26602870
http://dx.doi.org/10.1590/0074-02760150317
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