AB79. Establishment of the high resolution melting analysis with unlabeled probes for genotyping CCL2-2518 T>C

OBJECTIVE: To establish a high resolution melting analysis with unlabeled probes for genotyping CCL2-2518 T>C. METHODS: Two unlabeled probes were designed. One is complementary to wild type and another is complementary to the mutant type. 71 health people were performed by HRM with unlabeled prob...

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Autores principales: Lei, Hongen, Gao, Zhezhu, Xu, Yongde, Xin, Zhongcheng, Guo, Yinglu, Guan, Ruili
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2014
Materias:
Free Paper Presentation
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4708360/
http://dx.doi.org/10.3978/j.issn.2223-4683.2014.s079
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author Lei, Hongen
Gao, Zhezhu
Xu, Yongde
Xin, Zhongcheng
Guo, Yinglu
Guan, Ruili
author_facet Lei, Hongen
Gao, Zhezhu
Xu, Yongde
Xin, Zhongcheng
Guo, Yinglu
Guan, Ruili
author_sort Lei, Hongen
collection PubMed
description OBJECTIVE: To establish a high resolution melting analysis with unlabeled probes for genotyping CCL2-2518 T>C. METHODS: Two unlabeled probes were designed. One is complementary to wild type and another is complementary to the mutant type. 71 health people were performed by HRM with unlabeled probes for genotyping CCL2-2518 T>C, and TaqMan method was used to verify the results from above. RESULTS: The results using the HRM with unlabeled probes were consistent with that using TaqMan method. The difference between the temperature in two probe peaks by using the probe complementary to wild type is 2.1°C; and the difference between the temperature in two probe peaks by using the probe complementary to mutant type is 5.5°C. CONCLUSIONS: Establishment the high resolution melting analysis with unlabeled probes for genotyping CCL2-2518 T>C is a simple, economic, sensitive, accurate, and fast technique, which could be used in clinical inspection.
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spelling pubmed-47083602016-01-26 AB79. Establishment of the high resolution melting analysis with unlabeled probes for genotyping CCL2-2518 T>C Lei, Hongen Gao, Zhezhu Xu, Yongde Xin, Zhongcheng Guo, Yinglu Guan, Ruili Transl Androl Urol Free Paper Presentation OBJECTIVE: To establish a high resolution melting analysis with unlabeled probes for genotyping CCL2-2518 T>C. METHODS: Two unlabeled probes were designed. One is complementary to wild type and another is complementary to the mutant type. 71 health people were performed by HRM with unlabeled probes for genotyping CCL2-2518 T>C, and TaqMan method was used to verify the results from above. RESULTS: The results using the HRM with unlabeled probes were consistent with that using TaqMan method. The difference between the temperature in two probe peaks by using the probe complementary to wild type is 2.1°C; and the difference between the temperature in two probe peaks by using the probe complementary to mutant type is 5.5°C. CONCLUSIONS: Establishment the high resolution melting analysis with unlabeled probes for genotyping CCL2-2518 T>C is a simple, economic, sensitive, accurate, and fast technique, which could be used in clinical inspection. AME Publishing Company 2014-09 /pmc/articles/PMC4708360/ http://dx.doi.org/10.3978/j.issn.2223-4683.2014.s079 Text en 2014 Translational Andrology and Urology. All rights reserved.
spellingShingle Free Paper Presentation
Lei, Hongen
Gao, Zhezhu
Xu, Yongde
Xin, Zhongcheng
Guo, Yinglu
Guan, Ruili
AB79. Establishment of the high resolution melting analysis with unlabeled probes for genotyping CCL2-2518 T>C
title AB79. Establishment of the high resolution melting analysis with unlabeled probes for genotyping CCL2-2518 T>C
title_full AB79. Establishment of the high resolution melting analysis with unlabeled probes for genotyping CCL2-2518 T>C
title_fullStr AB79. Establishment of the high resolution melting analysis with unlabeled probes for genotyping CCL2-2518 T>C
title_full_unstemmed AB79. Establishment of the high resolution melting analysis with unlabeled probes for genotyping CCL2-2518 T>C
title_short AB79. Establishment of the high resolution melting analysis with unlabeled probes for genotyping CCL2-2518 T>C
title_sort ab79. establishment of the high resolution melting analysis with unlabeled probes for genotyping ccl2-2518 t>c
topic Free Paper Presentation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4708360/
http://dx.doi.org/10.3978/j.issn.2223-4683.2014.s079
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