AB187. Establishment of the high resolution melting analysis with unlabeled probes for genotyping CCL2-2518 T > C

OBJECTIVE: To establish a high resolution melting analysis with unlabeled probes for genotyping CCL2-2518 T > C. METHODS: Two unlabeled probes were designed. One is complementary to wild type and another is complementary to the mutant type. A total of 71 health people were performed by HRM with unlabeled probes for genotyping CCL2-2518 T > C, and TaqMan method was used to verify the results from above. RESULTS: The results using the HRM with unlabeled probes were consistent with that using TaqMan method. The difference between the temperatures in two probe peaks by using the probe complementary to wild type is 2.1°C; and the difference between the temperatures in two probe peaks by using the probe complementary to mutant type is 5.5°C. CONCLUSIONS: Establishment the high resolution melting analysis with unlabeled probes for genotyping CCL2-2518 T > C is a simple, economic, sensitive, accurate, and fast technique, which could be used in clinical inspection.