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AB178. Apocynin improves erectile function indiabetic rats through regulation of NADPH oxidase expression

INTRODUCTION: Diabetes is a risk factor for erectile dysfunction (ED). The proposed mechanisms responsible fordiabetic ED are associated with an increase in reactive oxygen species (ROS) production, over activity of RhoA/ROCK signaling pathway and nicotinamide adenine dinucleotide phosphate (NADPH)...

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Detalles Bibliográficos
Autores principales: Li, Mingchao, Zhuan, Li, Wang, Tao, Rao, Ke, Yang, Jun, Quan, Weihe, Liu, Jihong, Ye, Zhangqun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4708423/
http://dx.doi.org/10.3978/j.issn.2223-4683.2014.s178
Descripción
Sumario:INTRODUCTION: Diabetes is a risk factor for erectile dysfunction (ED). The proposed mechanisms responsible fordiabetic ED are associated with an increase in reactive oxygen species (ROS) production, over activity of RhoA/ROCK signaling pathway and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, as seen in experimental models of diabetic rats. AIM: The aim of this study was to investigate whether NADPH oxidase inhibitor apocynin can ameliorate streptozotocin (STZ)-induced diabetes-related ED by reducing the ROS production and inhibiting the activity of RhoA/ROCK signaling pathway. METHODS: The diabetic rats were treated with and without the NADPH oxidase inhibitor apocynin. MAIN OUTCOME MEASURES: Erectile responses were evaluated by determining mean arterial blood pressure (MAP) and intracavernosal pressure (ICP) with electrical stimulation of the cavernous nerve. Levels of mRNA expression were measured by real-time polymerase chain reaction (RT-PCR). Levels of protein expression were examined by Western Blot. ROS production was measured by dihydroethidium (DHE) staining and thiobarbituric acid reactive substances assay. RESULTS: The ratio of maximum ICP-to-MAP (max ICP/MAP) was significantly decreased in diabetic ED rats, compared to that of age-matched control rats (P<0.05). Apocynin improved erectile function of diabetic rats (P<0.05). Expression levels of RhoA (cytosol), nNOS and eNOS were reduced, compared to those of control rats (P<0.05). Apocynin significantly elevated their expression levels in diabetic rats (P<0.05). Expression levels of ROCK1, RhoA (membrane fraction), p-MYPT1 and NADPH oxidase subunits p47 (phox) and p67 (phox) were increased in diabetic rats when compared to those of control rats (P<0.05), and it was observed that apocynin significantly reduced their expression levels in diabetic rats (P<0.05). ROS production was increased in diabetic rats when compared to that of control rats (P<0.05), the effect of apocynin was a reduction in the ROS production in diabetic rats (P<0.05).