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AB139. Studies on the mechanism of testicular dysfunction in the early stage of a streptozotocin induced diabetic rat model

Streptozotocin (STZ) induced diabetic model has been widely used to study the effects of diabetes mellitus (DM) on male infertility, but it remains unclear whether the responses in this model are due to hyperglycemia or STZ per se. This study was designed to investigate the mechanism of STZ on testi...

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Autores principales: Xu, Yongde, Lei, Hongen, Guan, Ruili, Gao, Zhezhu, Li, Huixi, Wang, Lin, Song, Weidong, Gao, Bing, Xing, Zhongcheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4708511/
http://dx.doi.org/10.3978/j.issn.2223-4683.2014.s139
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author Xu, Yongde
Lei, Hongen
Guan, Ruili
Gao, Zhezhu
Li, Huixi
Wang, Lin
Song, Weidong
Gao, Bing
Xing, Zhongcheng
author_facet Xu, Yongde
Lei, Hongen
Guan, Ruili
Gao, Zhezhu
Li, Huixi
Wang, Lin
Song, Weidong
Gao, Bing
Xing, Zhongcheng
author_sort Xu, Yongde
collection PubMed
description Streptozotocin (STZ) induced diabetic model has been widely used to study the effects of diabetes mellitus (DM) on male infertility, but it remains unclear whether the responses in this model are due to hyperglycemia or STZ per se. This study was designed to investigate the mechanism of STZ on testicular dysfunction. In the present study, sperm characteristics, serum testosterone, steroidogenic enzymes (StAR and 3β-HSD), and the vimentin apical extension of sertoli cells decreased significantly in the STZ group compared with those in the normal controls (P<0.05), while Johnsen’s score, testicular lipid peroxidation, spermatogenic cell apoptosis, and the expressions of NF-κB and Wnt4 significantly increased (P<0.05). Insulin replacement mainly restored the decreased serum testosterone and steroidogenic enzymes, but not other parameters. The results indicated that spermatogenic dysfunction in the early stage of STZ-induced diabetic rats was due to direct STZ cytotoxity to sertoli cells, which could be regulated by Wnt4 and NF-κB, while steroidogenic dysfunction might be a direct or indirect consequence of insulin deficiency. The results suggested that STZ-induced diabetic model, at least in the early stage, is not suitable to study the diabetes-related spermatogenic dysfunction.
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spelling pubmed-47085112016-01-26 AB139. Studies on the mechanism of testicular dysfunction in the early stage of a streptozotocin induced diabetic rat model Xu, Yongde Lei, Hongen Guan, Ruili Gao, Zhezhu Li, Huixi Wang, Lin Song, Weidong Gao, Bing Xing, Zhongcheng Transl Androl Urol Abstract Publication Reproduction Streptozotocin (STZ) induced diabetic model has been widely used to study the effects of diabetes mellitus (DM) on male infertility, but it remains unclear whether the responses in this model are due to hyperglycemia or STZ per se. This study was designed to investigate the mechanism of STZ on testicular dysfunction. In the present study, sperm characteristics, serum testosterone, steroidogenic enzymes (StAR and 3β-HSD), and the vimentin apical extension of sertoli cells decreased significantly in the STZ group compared with those in the normal controls (P<0.05), while Johnsen’s score, testicular lipid peroxidation, spermatogenic cell apoptosis, and the expressions of NF-κB and Wnt4 significantly increased (P<0.05). Insulin replacement mainly restored the decreased serum testosterone and steroidogenic enzymes, but not other parameters. The results indicated that spermatogenic dysfunction in the early stage of STZ-induced diabetic rats was due to direct STZ cytotoxity to sertoli cells, which could be regulated by Wnt4 and NF-κB, while steroidogenic dysfunction might be a direct or indirect consequence of insulin deficiency. The results suggested that STZ-induced diabetic model, at least in the early stage, is not suitable to study the diabetes-related spermatogenic dysfunction. AME Publishing Company 2014-09 /pmc/articles/PMC4708511/ http://dx.doi.org/10.3978/j.issn.2223-4683.2014.s139 Text en 2014 Translational Andrology and Urology. All rights reserved.
spellingShingle Abstract Publication Reproduction
Xu, Yongde
Lei, Hongen
Guan, Ruili
Gao, Zhezhu
Li, Huixi
Wang, Lin
Song, Weidong
Gao, Bing
Xing, Zhongcheng
AB139. Studies on the mechanism of testicular dysfunction in the early stage of a streptozotocin induced diabetic rat model
title AB139. Studies on the mechanism of testicular dysfunction in the early stage of a streptozotocin induced diabetic rat model
title_full AB139. Studies on the mechanism of testicular dysfunction in the early stage of a streptozotocin induced diabetic rat model
title_fullStr AB139. Studies on the mechanism of testicular dysfunction in the early stage of a streptozotocin induced diabetic rat model
title_full_unstemmed AB139. Studies on the mechanism of testicular dysfunction in the early stage of a streptozotocin induced diabetic rat model
title_short AB139. Studies on the mechanism of testicular dysfunction in the early stage of a streptozotocin induced diabetic rat model
title_sort ab139. studies on the mechanism of testicular dysfunction in the early stage of a streptozotocin induced diabetic rat model
topic Abstract Publication Reproduction
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4708511/
http://dx.doi.org/10.3978/j.issn.2223-4683.2014.s139
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