Carboxylesterase converts Amplex red to resorufin: Implications for mitochondrial H(2)O(2) release assays

Amplex Red is a fluorescent probe that is widely used to detect hydrogen peroxide (H(2)O(2)) in a reaction where it is oxidised to resorufin by horseradish peroxidase (HRP) as a catalyst. This assay is highly rated amongst other similar probes thanks to its superior sensitivity and stability. Howeve...

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Detalles Bibliográficos
Autores principales: Miwa, Satomi, Treumann, Achim, Bell, Amy, Vistoli, Giulio, Nelson, Glyn, Hay, Sam, von Zglinicki, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science 2016
Materias:
Original Contribution
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4708625/
https://www.ncbi.nlm.nih.gov/pubmed/26577176
http://dx.doi.org/10.1016/j.freeradbiomed.2015.11.011

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4708625/
https://www.ncbi.nlm.nih.gov/pubmed/26577176
http://dx.doi.org/10.1016/j.freeradbiomed.2015.11.011

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