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Carboxylesterase converts Amplex red to resorufin: Implications for mitochondrial H(2)O(2) release assays
Amplex Red is a fluorescent probe that is widely used to detect hydrogen peroxide (H(2)O(2)) in a reaction where it is oxidised to resorufin by horseradish peroxidase (HRP) as a catalyst. This assay is highly rated amongst other similar probes thanks to its superior sensitivity and stability. Howeve...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier Science
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4708625/ https://www.ncbi.nlm.nih.gov/pubmed/26577176 http://dx.doi.org/10.1016/j.freeradbiomed.2015.11.011 |