Cargando…
AB160. Intracavernous transplantation of bone marrow-derived mesenchymal stem cells restores erectile function of streptozocin-induced diabetic rats
OBJECTIVE: To explore the effects of transplantation of bone marrow-derived mesenchymal stem cells (BM-MSCs) on improving erectile function of streptozocin (STZ)-induced diabetic rats. METHODS: Male Sprague Dawley rats were injected either with STZ to induce diabetes or with citrate buffer as contro...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AME Publishing Company
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4708731/ http://dx.doi.org/10.3978/j.issn.2223-4683.2015.s160 |
_version_ | 1782409532592357376 |
---|---|
author | Qiu, Xuefeng Dai, Yutian |
author_facet | Qiu, Xuefeng Dai, Yutian |
author_sort | Qiu, Xuefeng |
collection | PubMed |
description | OBJECTIVE: To explore the effects of transplantation of bone marrow-derived mesenchymal stem cells (BM-MSCs) on improving erectile function of streptozocin (STZ)-induced diabetic rats. METHODS: Male Sprague Dawley rats were injected either with STZ to induce diabetes or with citrate buffer as controls. Rat BM-MSCs were harvested and labeled with Chloromethyl Benzamido derivatives of 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate (CM-DiI), and then transplanted into corporal cavernosum of STZ-induced diabetic rats. Four weeks after transplantation, all rats were analyzed for erectile function and penile histology. RESULTS: After BM-MSCs transplantation, the ICP/MAP ratio was increased significantly compared with diabetic controls. Content of smooth muscle and endothelium in corporal cavernosa of BM-MSCs transplanted rats was significantly increased compared to diabetic controls. Immunofluorescence analysis demonstrated that CM-DiI-labeled BM-MSCs could stay in corporal cavernosa for at least 4 weeks and some of them expressed von Willebrand Factor, CD31, calponin, or α-smooth muscle actin, cells markers for endothelial cells or smooth muscle cells, respectively. CONCLUSIONS: Intracavernous transplantation of BM-MSCs had beneficial effects on erectile function of diabetic rats and increased the content of endothelium and smooth muscle in corporal cavernosum. |
format | Online Article Text |
id | pubmed-4708731 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | AME Publishing Company |
record_format | MEDLINE/PubMed |
spelling | pubmed-47087312016-01-26 AB160. Intracavernous transplantation of bone marrow-derived mesenchymal stem cells restores erectile function of streptozocin-induced diabetic rats Qiu, Xuefeng Dai, Yutian Transl Androl Urol Moderated Poster Presentation OBJECTIVE: To explore the effects of transplantation of bone marrow-derived mesenchymal stem cells (BM-MSCs) on improving erectile function of streptozocin (STZ)-induced diabetic rats. METHODS: Male Sprague Dawley rats were injected either with STZ to induce diabetes or with citrate buffer as controls. Rat BM-MSCs were harvested and labeled with Chloromethyl Benzamido derivatives of 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate (CM-DiI), and then transplanted into corporal cavernosum of STZ-induced diabetic rats. Four weeks after transplantation, all rats were analyzed for erectile function and penile histology. RESULTS: After BM-MSCs transplantation, the ICP/MAP ratio was increased significantly compared with diabetic controls. Content of smooth muscle and endothelium in corporal cavernosa of BM-MSCs transplanted rats was significantly increased compared to diabetic controls. Immunofluorescence analysis demonstrated that CM-DiI-labeled BM-MSCs could stay in corporal cavernosa for at least 4 weeks and some of them expressed von Willebrand Factor, CD31, calponin, or α-smooth muscle actin, cells markers for endothelial cells or smooth muscle cells, respectively. CONCLUSIONS: Intracavernous transplantation of BM-MSCs had beneficial effects on erectile function of diabetic rats and increased the content of endothelium and smooth muscle in corporal cavernosum. AME Publishing Company 2015-08 /pmc/articles/PMC4708731/ http://dx.doi.org/10.3978/j.issn.2223-4683.2015.s160 Text en 2015 Translational Andrology and Urology. All rights reserved. |
spellingShingle | Moderated Poster Presentation Qiu, Xuefeng Dai, Yutian AB160. Intracavernous transplantation of bone marrow-derived mesenchymal stem cells restores erectile function of streptozocin-induced diabetic rats |
title | AB160. Intracavernous transplantation of bone marrow-derived mesenchymal stem cells restores erectile function of streptozocin-induced diabetic rats |
title_full | AB160. Intracavernous transplantation of bone marrow-derived mesenchymal stem cells restores erectile function of streptozocin-induced diabetic rats |
title_fullStr | AB160. Intracavernous transplantation of bone marrow-derived mesenchymal stem cells restores erectile function of streptozocin-induced diabetic rats |
title_full_unstemmed | AB160. Intracavernous transplantation of bone marrow-derived mesenchymal stem cells restores erectile function of streptozocin-induced diabetic rats |
title_short | AB160. Intracavernous transplantation of bone marrow-derived mesenchymal stem cells restores erectile function of streptozocin-induced diabetic rats |
title_sort | ab160. intracavernous transplantation of bone marrow-derived mesenchymal stem cells restores erectile function of streptozocin-induced diabetic rats |
topic | Moderated Poster Presentation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4708731/ http://dx.doi.org/10.3978/j.issn.2223-4683.2015.s160 |
work_keys_str_mv | AT qiuxuefeng ab160intracavernoustransplantationofbonemarrowderivedmesenchymalstemcellsrestoreserectilefunctionofstreptozocininduceddiabeticrats AT daiyutian ab160intracavernoustransplantationofbonemarrowderivedmesenchymalstemcellsrestoreserectilefunctionofstreptozocininduceddiabeticrats |