Cargando…

AB185. Therapeutic potential of adipose-derived stem cells-based micro-tissues in a rat model of stress urinary incontinence

OBJECTIVE: Using adipose-derived stem cells (ADSCs) to halt stress urinary incontinence (SUI) progression and regenerate urethral sphincter (US) has emerged as a promising method to restore normal voiding function. However, traditional two-dimensional (2D) cell culture systems have limitations in mi...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Meng, Lei, Hongen, Yang, Bicheng, Guan, Ruili, Hui, Yu, Gao, Zhezhu, Li, Guangyong, Xin, Zhongcheng, Chen, Fubao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4708737/
http://dx.doi.org/10.3978/j.issn.2223-4683.2015.s185
_version_ 1782409533936631808
author Li, Meng
Lei, Hongen
Yang, Bicheng
Guan, Ruili
Hui, Yu
Gao, Zhezhu
Li, Guangyong
Xin, Zhongcheng
Chen, Fubao
author_facet Li, Meng
Lei, Hongen
Yang, Bicheng
Guan, Ruili
Hui, Yu
Gao, Zhezhu
Li, Guangyong
Xin, Zhongcheng
Chen, Fubao
author_sort Li, Meng
collection PubMed
description OBJECTIVE: Using adipose-derived stem cells (ADSCs) to halt stress urinary incontinence (SUI) progression and regenerate urethral sphincter (US) has emerged as a promising method to restore normal voiding function. However, traditional two-dimensional (2D) cell culture systems have limitations in mimicking in vivo conditions losing some important properties. This study aims to examine the potential and mechanism of three-dimensional (3D) cultures of ADSCs in the treatment of SUI in a rat model simulating childbirth injury. METHODS: ADSCs were used to generate micro-tissues (MTs) with a hanging drop method. A total of 48 postpartum Sprague-Dawley (SD) rats were developed SUI models by 4 h vagina dilation (VD) followed by bilateral ovariectomy (OV). Ten rats underwent sham OV without VD as control group. The SUI rats were divided into three groups and received urethral injection of PBS, ADSCs and MTs. specimens were harvested for histology and tracking CM-Dil-Labelled ADSCs at day 1, 3, 7 (n=3), and 28 (n=7) post-injection. At day 28, the remaining rats in each group were examined for voiding function. Western blot, immunofluorescence (IF) and immunohistochemistry (IHC) staining were performed to examine the histological change and cytokines. RESULTS: Compared with ADSCs, MTs expressed increased vascular endothelia growth factor (VEGF), TNFα stimulated gene/protein 6 (TSG-6) in vitro and represented a higher retention rate in vivo. The voiding function and histopathological recovery were significantly better in MTs group than ADSCs group. CONCLUSIONS: Urethral injection of MTs better restored the voiding function than ADSCs. The up-regulation of some bioactive factors and the improved cellular retention in the urethra may be responsible for the underlying mechanisms.
format Online
Article
Text
id pubmed-4708737
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher AME Publishing Company
record_format MEDLINE/PubMed
spelling pubmed-47087372016-01-26 AB185. Therapeutic potential of adipose-derived stem cells-based micro-tissues in a rat model of stress urinary incontinence Li, Meng Lei, Hongen Yang, Bicheng Guan, Ruili Hui, Yu Gao, Zhezhu Li, Guangyong Xin, Zhongcheng Chen, Fubao Transl Androl Urol Moderated Poster Presentation OBJECTIVE: Using adipose-derived stem cells (ADSCs) to halt stress urinary incontinence (SUI) progression and regenerate urethral sphincter (US) has emerged as a promising method to restore normal voiding function. However, traditional two-dimensional (2D) cell culture systems have limitations in mimicking in vivo conditions losing some important properties. This study aims to examine the potential and mechanism of three-dimensional (3D) cultures of ADSCs in the treatment of SUI in a rat model simulating childbirth injury. METHODS: ADSCs were used to generate micro-tissues (MTs) with a hanging drop method. A total of 48 postpartum Sprague-Dawley (SD) rats were developed SUI models by 4 h vagina dilation (VD) followed by bilateral ovariectomy (OV). Ten rats underwent sham OV without VD as control group. The SUI rats were divided into three groups and received urethral injection of PBS, ADSCs and MTs. specimens were harvested for histology and tracking CM-Dil-Labelled ADSCs at day 1, 3, 7 (n=3), and 28 (n=7) post-injection. At day 28, the remaining rats in each group were examined for voiding function. Western blot, immunofluorescence (IF) and immunohistochemistry (IHC) staining were performed to examine the histological change and cytokines. RESULTS: Compared with ADSCs, MTs expressed increased vascular endothelia growth factor (VEGF), TNFα stimulated gene/protein 6 (TSG-6) in vitro and represented a higher retention rate in vivo. The voiding function and histopathological recovery were significantly better in MTs group than ADSCs group. CONCLUSIONS: Urethral injection of MTs better restored the voiding function than ADSCs. The up-regulation of some bioactive factors and the improved cellular retention in the urethra may be responsible for the underlying mechanisms. AME Publishing Company 2015-08 /pmc/articles/PMC4708737/ http://dx.doi.org/10.3978/j.issn.2223-4683.2015.s185 Text en 2015 Translational Andrology and Urology. All rights reserved.
spellingShingle Moderated Poster Presentation
Li, Meng
Lei, Hongen
Yang, Bicheng
Guan, Ruili
Hui, Yu
Gao, Zhezhu
Li, Guangyong
Xin, Zhongcheng
Chen, Fubao
AB185. Therapeutic potential of adipose-derived stem cells-based micro-tissues in a rat model of stress urinary incontinence
title AB185. Therapeutic potential of adipose-derived stem cells-based micro-tissues in a rat model of stress urinary incontinence
title_full AB185. Therapeutic potential of adipose-derived stem cells-based micro-tissues in a rat model of stress urinary incontinence
title_fullStr AB185. Therapeutic potential of adipose-derived stem cells-based micro-tissues in a rat model of stress urinary incontinence
title_full_unstemmed AB185. Therapeutic potential of adipose-derived stem cells-based micro-tissues in a rat model of stress urinary incontinence
title_short AB185. Therapeutic potential of adipose-derived stem cells-based micro-tissues in a rat model of stress urinary incontinence
title_sort ab185. therapeutic potential of adipose-derived stem cells-based micro-tissues in a rat model of stress urinary incontinence
topic Moderated Poster Presentation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4708737/
http://dx.doi.org/10.3978/j.issn.2223-4683.2015.s185
work_keys_str_mv AT limeng ab185therapeuticpotentialofadiposederivedstemcellsbasedmicrotissuesinaratmodelofstressurinaryincontinence
AT leihongen ab185therapeuticpotentialofadiposederivedstemcellsbasedmicrotissuesinaratmodelofstressurinaryincontinence
AT yangbicheng ab185therapeuticpotentialofadiposederivedstemcellsbasedmicrotissuesinaratmodelofstressurinaryincontinence
AT guanruili ab185therapeuticpotentialofadiposederivedstemcellsbasedmicrotissuesinaratmodelofstressurinaryincontinence
AT huiyu ab185therapeuticpotentialofadiposederivedstemcellsbasedmicrotissuesinaratmodelofstressurinaryincontinence
AT gaozhezhu ab185therapeuticpotentialofadiposederivedstemcellsbasedmicrotissuesinaratmodelofstressurinaryincontinence
AT liguangyong ab185therapeuticpotentialofadiposederivedstemcellsbasedmicrotissuesinaratmodelofstressurinaryincontinence
AT xinzhongcheng ab185therapeuticpotentialofadiposederivedstemcellsbasedmicrotissuesinaratmodelofstressurinaryincontinence
AT chenfubao ab185therapeuticpotentialofadiposederivedstemcellsbasedmicrotissuesinaratmodelofstressurinaryincontinence